[1]底煜,陈晓隆.磷脂酰肌醇-3-激酶/丝氨酸-苏氨酸激酶抑制剂LY294002对小鼠视网膜新生血管形成的抑制作用[J].眼科新进展,2018,38(3):210-213.[doi:10.13389/j.cnki.rao.2018.0048]
　DI Yu,CHEN Xiao-Long.Inhibitory effects of PI3K/AKT inhibitor LY294002 on retinal neovascularization in mice[J].Recent Advances in Ophthalmology,2018,38(3):210-213.[doi:10.13389/j.cnki.rao.2018.0048]
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磷脂酰肌醇-3-激酶/丝氨酸-苏氨酸激酶抑制剂LY294002对小鼠视网膜新生血管形成的抑制作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年3期
页码:
210-213
栏目:
实验研究
出版日期:
2018-03-05

文章信息/Info

Title:
Inhibitory effects of PI3K/AKT inhibitor LY294002 on retinal neovascularization in mice
作者:
底煜; 陈晓隆
110004　辽宁省沈阳市，中国医科大学附属盛京医院眼科
Author(s):
DI Yu; CHEN Xiao-Long
Department of Ophthalmology，Shengjing Hospital of China Medical University，Shenyang 110004，Liaoning Province，China
关键词:
LY294002; 磷脂酰肌醇-3-激酶/丝氨酸-苏氨酸激酶; 氧诱导视网膜病变; 早产儿视网膜病变; 视网膜新生血管
Keywords:
LY294002; phosphatidylinositol-3-kinase/serine/threonine kinase; oxygen-induced retinopathy; retinopathy of prematurity; retinal neovascularization
分类号:
R774.1；R779.7
DOI:
10.13389/j.cnki.rao.2018.0048
文献标志码:
A
摘要:
目的　探讨磷脂酰肌醇-3-激酶（phosphatidylinositol-3-kinase，PI3K）/丝氨酸-苏氨酸激酶（Serine/threonine kinase，AKT）信号转导通路抑制剂LY294002对小鼠氧诱导视网膜病变（oxygen-induced retinopathy，OIR）的视网膜新生血管（retinal neovascularization，RNV）形成的影响。方法　取C57BL/6J小鼠60只，随机分为实验组和对照组，每组各30只，均制备OIR模型。小鼠出氧箱前1 d即鼠龄11 d时实验组玻璃体内注射0.5 μL的LY294002，对照组玻璃体内注射等体积的PBS。病理切片计数突破视网膜内界膜的新生血管内皮细胞核数，免疫组织化学和RT-PCR法检测pAKT、VEGF蛋白及mRNA的表达情况。结果　实验组小鼠新生血管内皮细胞核数为（12.53±1.71）个，较对照组（25.31±1.42）个明显减少（P<0.05）；实验组小鼠pAKT、VEGF的蛋白表达呈弱阳性，阳性细胞的吸光度值（9.12±1.35、13.91±1.49）均较对照组（15.11±2.17、19.72±2.61）明显下降（均为P<0.05）；实验组小鼠AKT、VEGF mRNA相对表达量均较对照组明显下降（均为P<0.05）。结论　LY294002通过抑制PI3K/AKT信号转导通路，可有效抑制小鼠OIR的RNV形成，LY294002有望成为防治血管增生性视网膜病变的一种有效方法。
Abstract:
Objective　To explore the effects of phosphatidylinositol-3-kinase （PI3K）/serine-threonine kinase （AKT） signal pathway inhibitor LY294002 on retinal neovascularization （RNV） in oxygen-induced retinopathy （OIR） mice.Methods　Totally 60 C57BL/6J mice were collected and randomly divided into the experimental group and control group，with 30 mice in each group.Then OIR model was induced by Smith methods.Rats in the experimental group were intravitreally injected with 0.5 μL LY294002，while the control group was given the same amount of phosphate buffer saline （PBS） one day before out of the incubator.Retinal sections with HE staining were applied to count the number of neovascular cell nuclei breaking through the inner limiting membrane，as well as the protein and mRNA expression of pAKT and vascular endothelial growth factor （VEGF） were detected by immunohistochemistry and RT-PCR.Results　The number of retinal neovascular cell nucleus in the experimental group was obviously smaller than that in the control group （P<0.05）.The protein expression of pAKT and VEGF was weakly expressed，and the absorbance （A） value of the positive cells was decreased in the experimental group compared with the control group （all P<0.05）.The mRNA expression of AKT and VEGF was obviously decreased in the experimental group compared with the control group （all P<0.05）.Conclusion　The development of RNV in OIR mice can be markedly inhibited by LY294002 inhibiting PI3K/AKT pathway，and therefore LY294002 is expected to be an effective method for preventing vascular proliferative retinopathy.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：81600747）；辽宁省博士启动基金资助（编号：201501020）
更新日期/Last Update: 2018-03-05