[1]孙一帆,王宵,杨诗巧,等.视觉发育关键期单眼剥夺对小鼠大脑不同区域星形胶质细胞的影响[J].眼科新进展,2024,44(7):517-521.[doi:10.13389/j.cnki.rao.2024.0099]
 SUN Yifan,WANG Xiao,YANG Shiqiao,et al.Effects of monocular deprivation during critical period of visual development on astrocytes in different regions of the mouse brain[J].Recent Advances in Ophthalmology,2024,44(7):517-521.[doi:10.13389/j.cnki.rao.2024.0099]
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视觉发育关键期单眼剥夺对小鼠大脑不同区域星形胶质细胞的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
44卷
期数:
2024年7期
页码:
517-521
栏目:
实验研究
出版日期:
2024-07-01

文章信息/Info

Title:
Effects of monocular deprivation during critical period of visual development on astrocytes in different regions of the mouse brain
作者:
孙一帆王宵杨诗巧王凯磊王雪纯陈亚敏张孝然吕盼盼郝瑞
300020 天津市,天津医科大学眼科临床学院(孙一帆,王宵,杨诗巧,王凯磊,王雪纯,陈亚敏,张孝然,郝瑞); 300020 天津市,天津市眼科医院(孙一帆,王宵,杨诗巧,王凯磊,王雪纯,陈亚敏,张孝然,吕盼盼,郝瑞);300020 天津市,南开大学附属眼科医院(吕盼盼,郝瑞)
Author(s):
SUN Yifan12WANG Xiao12YANG Shiqiao12WANG Kailei12WANG Xuechun12CHEN Yamin12ZHANG Xiaoran12L Panpan23HAO Rui123
1.Clinical College of Ophthalmology,Tianjin Medical University,Tianjin 300020,China
2.Tianjin Eye Hospital,Tianjin 300020,China
3.Nankai University Affiliated Eye Hospital,Tianjin 300020,China
关键词:
单眼剥夺视皮层上丘海马星形胶质细胞
Keywords:
monocular deprivation visual cortex superior colliculus hippocampus astrocyte
分类号:
R774.7
DOI:
10.13389/j.cnki.rao.2024.0099
文献标志码:
A
摘要:
目的 研究视觉发育关键期单眼剥夺(MD)对小鼠上丘、海马、视皮层星形胶质细胞表达的影响。
方法 选取C57BL/6J小鼠18只,随机分为正常对照组(CON组)和MD组,每组各9只,在12 h/12 h昼夜循环环境中饲养。CON组不做处理,MD组小鼠在生后27 d右眼行MD,7 d后取材。逆转录实时荧光定量聚合酶链式反应(RT-qPCR)检测两组小鼠上丘、海马、视皮层胶质纤维酸性蛋白(GFAP)mRNA表达情况。Western blot检测两组小鼠上丘、海马、视皮层GFAP蛋白表达的变化。免疫荧光染色检测两组小鼠上丘、海马、视皮层GFAP和中枢神经特异性蛋白(S100β)共定位标记的星形胶质细胞数量。
结果 RT-qPCR和Western blot检测结果显示,与CON组相比,MD组小鼠上丘、海马(锥体细胞1区、锥体细胞3区、齿状回区)、视皮层GFAP mRNA和蛋白表达均减少,差异均有统计学意义(均为P<0.05)。免疫荧光染色结果显示,与CON组相比,MD组小鼠上丘、海马(锥体细胞1区、锥体细胞3区、齿状回区)、视皮层GFAP和S100β共定位标记的星形胶质细胞数量均减少,差异均有统计学意义(均为P<0.05)。
结论 视觉发育关键期对小鼠进行MD导致其上丘、海马、视皮层星形胶质细胞数量减少。
Abstract:
Objective To explore the effects of monocular deprivation (MD) on the expression of astrocytes in the superior colliculus, hippocampus, and visual cortex in mice during the critical period of visual development.
Methods Eighteen C57BL/6J mice were randomly divided into the normal control group (CON group) and the MD group, with 9 mice in each group. Mice were bred under the 12 h/12 h dark/light conditions. Mice in the CON group received no treatment, while mice in the MD group underwent MD of the right eye on postnatal day 27, and the tissue was removed after 7 days. The mRNA and protein expression levels of glial fibrillary acidic protein (GFAP) in the superior colliculus, hippocampus and visual cortex of mice in the two groups were detected using the real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot, respectively. The number of astrocytes labeled by GFAP and central nervous system specific protein β (S100β) in the superior colliculus, hippocampus and visual cortex of mice in the two groups was detected using the immunofluorescence staining.
Results RT-qPCR and Western blot results showed that compared with the CON group, the mRNA and protein expression levels of GFAP in the superior colliculus, hippocampus (CA1, CA3 and dentate gyrus) and visual cortex of mice in the MD group decreased, and the differences were statistically significant (all P<0.05). The immunofluorescence staining results showed that compared with the CON group, the number of GFAP and S100β co-labeled astrocytes in the superior colliculus, hippocampus (CA1, CA3 and dentate gyrus) and visual cortex of mice in the MD group decreased, and the differences were statistically significant (all P<0.05).
Conclusion MD of mice during the critical period of visual development can result in a decrease in the number of astrocytes in the superior colliculus, hippocampus and visual cortex.

参考文献/References:

[1] HASEL P,LIDDELOW S A.Astrocytes[J].Curr Biol,2021,31(7):R326-R327.
[2] BUSHONG E A,MARTONE M E,JONES Y Z,ELLISMAN M H.Protoplasmic astrocytes in CA1 stratum radiatum occupy separate anatomical domains[J].J Neurosci,2002,22(1):183-192.
[3] ESPRITO-SANTO S,COUTINHO V G,DEZONNE R S,STIPURSKY J,DOS SANTOS-RODRIGUES A,BATISTA C,et al.Astrocytes as a target for Nogo-A and implications for synapse formation in vitro and in a model of acute demyelination[J].Glia,2021,69(6):1429-1443.
[4] ISMAIL F Y,FATEMI A,JOHNSTON M V.Cerebral plasticity:windows of opportunity in the developing brain[J].Eur J Paediatr Neurol,2017,21(1):23-48.
[5] MORISHITA H,HENSCH T K.Critical period revisited:impact on vision[J].Curr Opin Neurobiol,2008,18(1):101-107.
[6] GORDON J A,STRYKER M P.Experience-dependent plasticity of binocular responses in the primary visual cortex of the mouse[J].J Neurosci,1996,16(10):3274-3286.
[7] FAGIOLINI M,HENSCH T K.Inhibitory threshold for critical-period activation in primary visual cortex[J].Nature,2000,404(6774):183-186.
[8] GREIFZU F,PIELECKA-FORTUNA J,KALOGERAKI E,KREMPLER K,FAVARO P D,SCHLTER O M,et al.Environmental enrichment extends ocular dominance plasticity into adulthood and protects from stroke-induced impairments of plasticity[J].Proc Natl Acad Sci U S A,2014,111(3):1150-1155.
[9] HOFER S B,MRSIC-FLOGEL T D,BONHOEFFER T,HBENER M.Prior experience enhances plasticity in adult visual cortex[J].Nat Neurosci,2006,9(1):127-132.
[10] MAHMOUD S,GHARAGOZLOO M,SIMARD C,GRIS D.Astrocytes maintain glutamate homeostasis in the CNS by controlling the balance between glutamate uptake and release[J].Cells,2019,8(2):184.
[11] GRIBIZIS A,GE X,DAIGLE T L,ACKMAN J B,ZENG H,LEE D,et al.Visual cortex gains independence from peripheral drive before eye opening[J].Neuron,2019,104(4):711-723.e3.
[12] ROCHEFORT N L,GARASCHUK O,MILOS R I,NARUSHIMA M,MARANDI N,PICHLER B,et al.Sparsification of neuronal activity in the visual cortex at eye-opening[J].Proc Natl Acad Sci U S A,2009,106(35):15049-15054.
[13] WALLACE M T,PERRAULT T J Jr,HAIRSTON W D,STEIN B E.Visual experience is necessary for the development of multisensory integration[J].J Neurosci,2004,24(43):9580-9584.
[14] BURROWS E L,MCOMISH C E,BURET L S,VAN DEN BUUSE M,HANNAN A J.Environmental enrichment ameliorates behavioral impairments modeling schizophrenia in mice lacking metabotropic glutamate receptor 5[J].Neuropsychopharmacology,2015,40(8):1947-1956.
[15] ARGANDOA E G,ROSSI M L,LAFUENTE J V.Visual deprivation effects on the s100beta positive astrocytic population in the developing rat visual cortex:a quantitative study[J].Brain Res Dev Brain Res,2003,141(1-2):63-69.
[16] MLLER C M.Dark-rearing retards the maturation of astrocytes in restricted layers of cat visual cortex[J].Glia,1990,3(6):487-494.
[17] STEWART M G,BOURNE R C,GABBOTT P L.Decreased levels of an astrocytic marker,glial fibrillary acidic protein,in the visual cortex of dark-reared rats:measurement by enzyme-linked immunosorbent assay[J].Neurosci Lett,1986,63(2):147-152.
[18] WANG Y,FU A K Y,IP N Y.Instructive roles of astrocytes in hippocampal synaptic plasticity:neuronal activity-dependent regulatory mechanisms[J].FEBS J,2022,289(8):2202-2218.
[19] LAVENEX P,AMARAL D G.Hippocampal-neocortical interaction:a hierarchy of associativity[J].Hippocampus,2000,10(4):420-430.
[20] CORVETTI L,AZTIRIA E,DOMENICI L.Reduction of GFAP induced by long dark rearing is not restricted to visual cortex[J].Brain Res,2006,1067(1):146-153.
[21] ALLEN N J,EROGLU C.Cell biology of astrocyte-synapse interactions[J].Neuron,2017,96(3):697-708.
[22] WANG L L,XU D,LUO Y,LI X,GU Y,WANG L.Homeostatic regulation of astrocytes by visual experience in the developing primary visual cortex[J].Cereb Cortex,2022,32(5):970-986.

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备注/Memo

备注/Memo:
国家自然科学基金项目(编号:81800861);天津市眼科医院科技基金青年培育项目(编号:YKPY2201);南开大学视光科学研究院开放基金一般项目(编号:NKSGY202309);天津市卫生健康行业高层次人才计划(青年医学新锐)(编号:TJSQNYXXR-D2-137);天津市卫生健康科技基金面上项目(编号:TJWJ2021MS041);天津市医学重点学科(专科)建设项目(编号:TJYXZDXK-016A)
更新日期/Last Update: 2024-07-05