[1]田笑雨,李婷,张盛楠,等.汉黄芩素对青光眼小鼠视网膜神经节细胞的保护作用及其机制研究[J].眼科新进展,2024,44(6):438-442.[doi:10.13389/j.cnki.rao.2024.0085]
 TIAN Xiaoyu,LI Ting,ZHANG Shengnan,et al.Protective effect of wogonin on retinal ganglion cells in mice with glaucoma and its mechanism[J].Recent Advances in Ophthalmology,2024,44(6):438-442.[doi:10.13389/j.cnki.rao.2024.0085]
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汉黄芩素对青光眼小鼠视网膜神经节细胞的保护作用及其机制研究/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
44卷
期数:
2024年6期
页码:
438-442
栏目:
实验研究
出版日期:
2024-06-03

文章信息/Info

Title:
Protective effect of wogonin on retinal ganglion cells in mice with glaucoma and its mechanism
作者:
田笑雨李婷张盛楠戴林莉
061000 河北省沧州市,沧州市中心医院眼科(田笑雨,李婷,张盛楠);100020 北京市,首都医科大学附属北京朝阳医院眼科(戴林莉)
Author(s):
TIAN Xiaoyu1LI Ting1ZHANG Shengnan1DAI Linli2
1.Department of Ophthalmology,Cangzhou Central Hospital,Cangzhou 061000,Hebei Province,China
2.Department of Ophthalmology,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China
关键词:
汉黄芩素青光眼视网膜神经节细胞氧化应激炎症反应
Keywords:
wogonin glaucoma retinal ganglion cells oxidative stress inflammatory reaction
分类号:
R775.5
DOI:
10.13389/j.cnki.rao.2024.0085
文献标志码:
A
摘要:
目的 观察汉黄芩素对青光眼小鼠视网膜神经节细胞(RGC)的保护作用,并探究其相关作用机制。
方法 10只(10眼)C57BL/6J小鼠作为对照组;40只(40眼)DBA/2J小鼠用于制备青光眼模型,并分为模型组和实验1、2、3组,每组各10只。对照组、模型组小鼠灌胃生理盐水,实验1、2、3组小鼠分别灌胃50、100、150 mg·kg-1汉黄芩素。以小鼠右眼为入选眼,比较各组小鼠灌胃前和灌胃2周、4周、8周眼压;苏木素-伊红染色并比较各组小鼠RGC数量、视网膜厚度;比色法检测各组小鼠视网膜组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮合酶(NOS)水平;酶联免疫吸附法检测各组小鼠视网膜组织白细胞介素(IL)-6、IL-1β、肿瘤坏死因子-α(TNF-α)水平;Western blot检测各组小鼠视网膜组织核苷酸寡聚化结构域样受体家族3(NLRP3)、半胱天冬酶-1(Caspase-1)、凋亡相关斑点样蛋白(ASC)的蛋白表达水平。
结果 灌胃前,与对照组相比,模型组和实验1、2、3组小鼠眼压均升高,差异均有统计学意义(均为P<0.05)。灌胃2周、4周、8周:与对照组相比,模型组和实验1、2、3组小鼠眼压均增加,差异均有统计学意义(均为P<0.05);与模型组相比,实验1、2、3组小鼠眼压均降低,差异均有统计学意义(均为P<0.05);与实验1组相比,实验2、3组小鼠眼压均降低,差异均有统计学意义(均为P<0.05);与实验2组相比,实验3组小鼠眼压均降低,差异均有统计学意义(均为P<0.05)。与对照组相比,模型组和实验1、2、3组小鼠RGC数量及视网膜厚度、SOD水平、GSH-Px水平均降低,NOS、IL-6、IL-1β、TNF-α水平以及NLRP3蛋白、Caspase-1蛋白、ASC蛋白表达水平均增加,差异均有统计学意义(均为P<0.05);与模型组相比,实验1、2、3组小鼠RGC数量及视网膜厚度、SOD水平、GSH-Px水平均增加,NOS、IL-6、IL-1β、TNF-α水平以及NLRP3蛋白、Caspase-1蛋白、ASC蛋白表达水平均降低,差异均有统计学意义(均为P<0.05)。与实验1组相比,实验2、3组小鼠RGC数量及视网膜厚度、SOD水平、GSH-Px水平均增加,NOS、IL-6、IL-1β、TNF-α水平以及NLRP3蛋白、Caspase-1蛋白、ASC蛋白表达水平均降低,差异均有统计学意义(均为P<0.05);与实验2组相比,实验3组小鼠RGC数量及视网膜厚度、SOD水平、GSH-Px水平均增加,NOS、IL-6、IL-1β、TNF-α水平以及NLRP3蛋白、Caspase-1蛋白、ASC蛋白表达水平均降低,差异均有统计学意义(均为P<0.05)。
结论 汉黄芩素可改善青光眼小鼠视网膜厚度,缓解视网膜组织氧化损伤和炎症反应,其作用机制可能与抑制NLRP3炎性小体激活有关。
Abstract:
Objective To explore the protective effect of wogonin on retinal ganglion cells (RGC) in mice with glaucoma and its related mechanism.
Methods Ten (10 eyes) C57BL/6J mice were included in the control group, and 40 (40 eyes) DBA/2J mice were selected to prepare glaucoma models, which were divided into the model group, experiment 1, 2 and 3 groups, with 10 mice per group. Mice in the control and model groups were given normal saline intragastrically, and mice in the experiment 1, 2 and 3 groups were intragastrically given 50, 100 and 150 mg·kg-1 wogonin, respectively. The right eyes were selected as experimental eyes. The intraocular pressure (IOP) of mice was compared among the five groups before and after 2, 4 and 8 weeks of intragastric administration. The RGC number and retinal thickness of mice in each group were compared after hematoxylin-eosin staining. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and nitric oxide synthase (NOS) in the retinal tissues of mice were detected by colorimetry. The levels of interleukin (IL)-6, IL-1β and tumor necrosis factor-α (TNF-α) in the retinal tissues of mice were detected by enzyme-linked immunosorbent assay. The expression levels of nucleotide oligomerization domain-like receptor family 3 (NLRP3), Caspase-1 and apoptosis-associated speck-like protein (ASC) in the retinal tissues of mice were detected by Western blot.
Results Compared with the control group, IOP in the model group and the experiment 1, 2 and 3 groups increased (all P<0.05) before intragastric administration. After 2, 4 and 8 weeks of intragastric administration, IOP in the model group and the experiment 1, 2 and 3 groups increased compared with the control group (all P<0.05); IOP in the experiment 1, 2 and 3 groups decreased compared with the model group (all P<0.05); IOP in the experiment 2 and 3 groups decreased compared with the experiment 1 group (both P<0.05); IOP in the experiment 3 group decreased compared with the experiment 2 group (P<0.05). Compared with the control group, the number of RGC, retinal thickness, SOD and GSH-Px levels in the model group and the experiment 1, 2 and 3 groups decreased, while the expression levels of NOS, IL-6, IL-1β, TNF-α, and NLRP3, Caspase-1 and ASC proteins increased, and the differences were statistically significant (all P<0.05). Compared with the model group, the number of RGC, retinal thickness, SOD and GSH-Px levels in the experiment 1, 2 and 3 groups increased, while the expression levels of NOS, IL-6, IL-1β, TNF-α, and NLRP3, Caspase-1 and ASC proteins decreased, and the differences were statistically significant (all P<0.05). Compared with the experiment 1 group, the number of RGC, retinal thickness, SOD and GSH-Px levels in the experiment 2 and 3 groups increased, while the expression levels of NOS, IL-6, IL-1β, TNF-α, and NLRP3, Caspase-1 and ASC proteins decreased (all P<0.05); compared with the experiment 2 group, the number of RGC, retinal thickness, SOD and GSH-Px levels in the experiment 3 group increased, while the expression levels of NOS, IL-6, IL-1β, TNF-α, and NLRP3, Caspase-1 and ASC proteins decreased (all P<0.05).
Conclusion Wogonin can improve the retinal thickness of mice with glaucoma and alleviate retinal oxidative damage and inflammatory reaction, which may be related to inhibiting the activation of NLRP3 inflammasomes.

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更新日期/Last Update: 2024-06-05