[1]舒会叶,韦芊,邵毅.视网膜类器官3D培养技术的研究进展[J].眼科新进展,2022,42(10):824-828.[doi:10.13389/j.cnki.rao.2022.0170]
　SHU Huiye,WEI Qian,SHAO Yi.Advances in the study of 3D culture technology for retinal organoids[J].Recent Advances in Ophthalmology,2022,42(10):824-828.[doi:10.13389/j.cnki.rao.2022.0170]
点击复制

视网膜类器官3D培养技术的研究进展/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
42卷
期数:
2022年10期
页码:
824-828
栏目:
文献综述
出版日期:
2022-10-05

文章信息/Info

Title:
Advances in the study of 3D culture technology for retinal organoids
作者:
舒会叶; 韦芊; 邵毅
330006　江西省南昌市，南昌大学第一附属医院眼科
Author(s):
SHU Huiye; WEI Qian; SHAO Yi
Department of Ophthalmology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,China
关键词:
视网膜退行性疾病; 视网膜移植; 类器官; 光感受器
Keywords:
retinal degenerative disease;  retinal transplantation;  organoid;  photoreceptor
分类号:
R774.5
DOI:
10.13389/j.cnki.rao.2022.0170
文献标志码:
A
摘要:
视网膜退行性疾病引起的光感受器细胞损伤会导致患者视力受损，出现进行性视野缩小、视野缺损等临床症状。由于视网膜的免疫赦免特性，异体光感受器移植成为治疗视网膜退行性疾病的潜在方法，但光感受器的组织来源又成为一大难题。人胚胎干细胞是由3D培养诱导分化而成的视网膜类器官，可以作为视网膜组织来源之一，为类器官移植提供了供体材料。本文就3D培养技术在视网膜类器官培养中的应用、类器官移植存在的问题、类器官与临床应用的距离等展开综述。
Abstract:
Photoreceptor cell injury caused by retinal degenerative diseases can lead to visual impairment, representing progressive visual field reduction, visual field defect, and other clinical manifestations. In consideration of the immune privilege properties of the retina, allogeneic photoreceptor implantation has become a potential treatment for retinal degenerative diseases. However, the source of tissue of photoreceptors has become a significant problem. As a retinal organoid induced by 3D culture, human embryonic stem cells can be used as one of the sources of retinal tissue, providing donor material for organoid transplantation. In this paper, the application of 3D culture technology to the cultivation of retinal organoids, the problems in organoid transplantation, and how far the organoids can be put into the clinical application are reviewed.

参考文献/References:

［1］　NICKERSON P,ORTIN-MARTINEZ A,WALLACE V A.Material exchange in photoreceptor transplantation:updating our understanding of donor/host communication and the future of cell engraftment science ［J］.Front Neural Circuits,2018,12:17.
［2］　REH T.Photoreceptor transplantation in late stage retinal degeneration ［J］.Invest Ophthalmol Vis Sci,2016,57(5):ORSFg1-ORSFg7.
［3］　SUGIMOTO S,SATO T.Establishment of 3D intestinal organoid cultures from intestinal stem cells ［J］.Methods Mol Biol,2017,1612:97-105.
［4］　MARIANI J,VACCARINO F M.Breakthrough moments:Yoshiki sasai’s discoveries in the third dimension ［J］.Cell Stem Cell,2019,24(6):837-838.
［5］　OBOKATA H,SASAI Y,NIWA H,KADOTA M,ANDRABI M,TAKATA N,et al.Retraction:bidirectional developmental potential in reprogrammed cells with acquired pluripotency ［J］.Nature,2014,511(757):112.
［6］　EIRAKU M,SASAI Y.Self-organizing optic-cup morphogenesis in three-dimensional culture ［J］.Neurosci Res,2011,71(S):E127-E128.
［7］　FUHRMANN S.Eye morphogenesis and patterning of the optic vesicle ［J］.Curr Top Dev Biol,2010,93:61-84.
［8］　WESTENSKOW P D,MCKEAN J B,KUBO F,NAKAGAWA S,FUHRMANN S.Ectopic Mitf in the embryonic chick retina by co-transfection of β-catenin and Otx2 ［J］.Invest Ophthalmol Vis Sci,2010,51(10):5328-5335.
［9］　STERN J H,TIAN Y,FUNDERBURGH J,PELLEGRINI G,ZHANG K,GOLDBERG J L,et al.Regenerating eye tissues to preserve and restore vision ［J］.Cell Stem Cell,2018,23(3):453.
［10］　VLKNER M,ZSCHTZSCH M,ROSTOVSKAYA M,OVERALL R W,BUSSKAMP V,ANASTASSIADIS K,et al.Retinal organoids from pluripotent stem cells efficiently recapitulate retinogenesis ［J］.Stem Cell Reports,2016,6(4):525-538.
［11］　OKUDA S,INOUE Y,EIRAKU M,SASAI Y,ADACHI T.Modeling cell proliferation for simulating three-dimensional tissue morphogenesis based on a reversible network reconnection framework ［J］.Biomech Model Mechanobiol,2013,12(5):987-996.
［12］　SASAI Y,EIRAKU M,SUGA H.In vitro organogenesis in three dimensions:self-organising stem cells ［J］.Development,2012,139(22):4111-4121.
［13］　DUTTA D,HEO I,CLEVERS H.Disease modeling in stem Cell-Derived 3D organoid systems ［J］.Trends Mol Med,2017,23(5):393-410.
［14］　BLONDEL D,LUTOLF M P.Bioinspired hydrogels for 3D organoid culture ［J］.Chimia (Aarau),2019,73(1):81-85.
［15］　BOONEKAMP K E,KRETZSCHMAR K,WIENER D J,ASRA P,DERAKHSHAN S,PUSCHHOF J,et al.Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures ［J］.Proc Natl Acad Sci U S A,2019,116(29):14630-14638.
［16］　OHLEMACHER S K,IGLESIAS C L,SRIDHAR A,GAMM D M,MEYER J S.Generation of highly enriched populations of optic vesicle-like retinal cells from human pluripotent stem cells ［J］.Curr Protoc Stem Cell Biol,2015,32:1H.8.1-1H.8.20.
［17］　DEKKERS J F,ALIEVA M,WELLENS L M,ARIESE H,JAMIESON P R,VONK A M,et al.High-resolution 3D imaging of fixed and cleared organoids ［J］.Nat Protoc,2019,14(6):1756-1771.
［18］　GARRETA E,KAMM R D,CHUVA D S,LANCASTER M A,WEISS R,TREPAT X,et al.Rethinking organoid technology through bioengineering ［J］.Nat Mater,2021,20(2):145-155.
［19］　QIAN X,SONG H,MING G L.Brain organoids:advances,applications and challenges ［J］.Development,2019,146(8):dev166074.
［20］　SKARDAL A,ALEMAN J,FORSYTHE S,RAJAN S,MURPHY S,DEVARASETTY M,et al.Drug compound screening in single and integrated multi-organoid body-on-a-chip systems ［J］.Biofabrication,2020,12(2):025017.
［21］　LLONCH S,CARIDO M,ADER M.Organoid technology for retinal repair ［J］.Dev Biol,2018,433(2):132-143.
［22］　HU H,GEHART H,ARTEGIANI B,LPEZ-IGLESIAS C,DEKKERS F,BASAK O,et al.Long-Term expansion of functional mouse and human hepatocytes as 3D organoids ［J］.Cell,2018,175(6):1591-1606.e19.
［23］　LEE C T,BENDRIEM R M,WU W W,SHEN R F.3D brain organoids derived from pluripotent stem cells:promising experimental models for brain development and neurodegenerative disorders ［J］.J Biomed Sci,2017,24(1):59.
［24］　LANCASTER M A,KNOBLICH J A.Organogenesis in a dish:modeling development and disease using organoid technologies ［J］.Science,2014,345(6194):1247125.
［25］　KRUCZEK K,SWAROOP A.Pluripotent stem cell-derived retinal organoids for disease modeling and development of therapies ［J］.Stem Cells,2020,38(10):1206-1215.
［26］　CAPOWSKI E E,SAMIMI K,MAYERL S J,PHILLIPS M J,PINILLA I,HOWDEN S E,et al.Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines ［J］.Development,2019,146(1):dev171686.
［27］　JIN Z B,GAO M L,DENG W L,WU K C,SUGITA S,MANDAI M,et al.Stemming retinal regeneration with pluripotent stem cells ［J］.Prog Retin Eye Res,2019,69:38-56.
［28］　BELL C M,ZACK D J,BERLINICKE C A.Human organoids for the study of retinal development and disease ［J］.Annu Rev Vis Sci,2020 ,6:91-114.
［29］　WU N,DOORENBOS M,CHEN D F.Induced pluripotent stem cells:development in the ophthalmologic field ［J］.Stem Cells Int,2016,2016:2361763.
［30］　FIELDS M A,BOWREY H E,GONG J,ABLONCZY Z,DEL PRIORE L V.Retinoid processing in induced pluripotent stem Cell-Derived retinal pigment epithelium cultures ［J］.Prog Mol Biol Transl Sci,2015,134:477-490.
［31］　MANAFI N,SHOKRI F,ACHBERGER K,HIRAYAMA M,MOHAMMADI M H,NOORIZADEH F,et al.Organoids and organ chips in ophthalmology ［J］.Ocul Surf,2021,19:1-15.
［32］　SHARMA K,KROHNE T,BUSSKAMP V.The rise of retinal organoids for vision research ［J］.Int J Mol Sci,2020,21(22):8484.

相似文献/References:

[1]陈娟 马晓晔.视网膜色素变性治疗最新进展[J].眼科新进展,2012,32(2):000.
[2]万光明,余川.重视不同来源移植细胞在视网膜退行性疾病中的替代治疗作用[J].眼科新进展,2022,42(8):589.[doi:10.13389/j.cnki.rao.2022.0121]
　WAN Guangming,YU Chuan.Effect of the replacement therapy of transplanted cells from different sources in retinal degenerative diseases[J].Recent Advances in Ophthalmology,2022,42(10):589.[doi:10.13389/j.cnki.rao.2022.0121]
[3]张海燕,莫亚.小胶质细胞极化在视网膜退行性疾病发病过程中的作用研究进展[J].眼科新进展,2023,43(12):997.[doi:10.13389/j.cnki.rao.2023.0198]
　ZHANG Haiyan,MO Ya.Advances in the role of microglia polarization in the pathogenesis of retinal degenerative diseases[J].Recent Advances in Ophthalmology,2023,43(10):997.[doi:10.13389/j.cnki.rao.2023.0198]

备注/Memo

备注/Memo:
国家自然科学基金项目（编号:82160195）；中央引导地方科技发展资金项目（编号:20211ZDG02003）
更新日期/Last Update: 2022-10-05