[1]王晓伟,李正日,金花,等.干眼患者泪液中Nod样受体1表达水平及其与临床指标的相关性[J].眼科新进展,2022,42(10):820-823.[doi:10.13389/j.cnki.rao.2022.0169]
　WANG Xiaowei,LI Zhengri,JIN Hua,et al.Analysis on the expression level of Nod-like receptor in tears of patients with dry eye and its correlation with various clinical parameters[J].Recent Advances in Ophthalmology,2022,42(10):820-823.[doi:10.13389/j.cnki.rao.2022.0169]
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干眼患者泪液中Nod样受体1表达水平及其与临床指标的相关性/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
42卷
期数:
2022年10期
页码:
820-823
栏目:
应用研究
出版日期:
2022-10-05

文章信息/Info

Title:
Analysis on the expression level of Nod-like receptor in tears of patients with dry eye and its correlation with various clinical parameters
作者:
王晓伟; 李正日; 金花; 贾寓洁; 赵景华; 赵健; 黄旭; 吴成哲
133000　吉林省延吉市，延边大学附属医院眼科
Author(s):
WANG Xiaowei; LI Zhengri; JIN Hua; JIA Yujie; ZHAO Jinghua; ZHAO Jian; HUANG Xu; WU Chengzhe
Department of Ophthalmology,the Affiliated Hospital of Yanbian University,Yanji 133000,Jilin Province,China
关键词:
干眼; 核苷酸结合寡聚化结构域样受体1; 核因子; 炎症
Keywords:
dry eye;  nucleotide-binding oligomerization domain-like receptor 1;  nuclear factor;  inflammation
分类号:
R777.34
DOI:
10.13389/j.cnki.rao.2022.0169
文献标志码:
A
摘要:
目的　探究核苷酸结合寡聚化结构域样受体1(Nod-1)在人泪液中的表达水平及其与临床干眼的相关性。方法　选取2021年1月至2021年6月就诊于延边大学附属医院眼科门诊受试者50名（右眼），其中干眼患者27眼（干眼组），健康人23眼（对照组）。通过分析眼表疾病指数（OSDI）问卷评分、泪膜破裂时间（BUT）、泪液分泌实验（SⅠT）、角膜荧光素染色（FL）评分以及泪液中Nod-1受体蛋白、核因子kappa B p65（NF-κB p65）蛋白和白细胞介素-1β（IL-1β）的表达水平，确定Nod-1受体蛋白和眼表参数之间的相关性。结果　干眼组和对照组受试者的OSDI评分、BUT、SⅠT、FL评分差异均有统计学意义(均为P<0.001)。干眼组患者的Nod-1受体蛋白、NF-κB p65蛋白、IL-1β表达水平均显著高于对照组，差异均有统计学意义（均为P<0.001）。干眼组患者中，Nod-1受体蛋白、NF-κB p65蛋白、IL-1β的表达水平与OSDI评分、FL评分均呈正相关性(均为P<0.05)，与SⅠT均呈负相关性(均为P<0.05)，与BUT均无明显相关性(均为P>0.05)。结论　干眼患者泪液中Nod-1受体蛋白表达与干眼严重程度相关。Nod-1受体蛋白、NF-κB p65蛋白和IL-1β共同参与了干眼的发生，Nod-1受体蛋白的表达可能在启动干眼的炎症反应中起重要作用。
Abstract:
Objective　To explore the expression level of nucleotide-binding oligomerization domain-like receptor 1 (Nod-1) in human tears and its correlation with dry eyes (DE). Methods　Totally 50 patients (right eyes) in the Ophthalmology Outpatient Department of Yanbian University Hospital from January 2021 to June 2021 were selected, including 27 patients in the DE group and 23 patients in the control group. The ocular surface disease index (OSDI) score, tear film breakup time (BUT), Schirmer I test (SIT), corneal fluorescein staining (FL), and the levels of Nod-1, nuclear factor kappa B p65 (NF-κB p65) and interleukin-1β (IL-1β) in tears were analyzed to determine the correlation between Nod-1 and ocular surface parameters. Results　The OSDI scores, BUT, SIT scores, and FL scores were significantly different between the DE group and the control group (all P<0.001). The expression levels of Nod-1, NF-κB p65 and IL-1β of the DE group were significantly higher than those in the control group (all P<0.0001). In the DE group, the expression levels of Nod-1, NF-κB p65 and IL-1β were positively correlated with OSDI and FL scores (all P<0.05), negatively correlated with SIT score (all P<0.05) and had no significant correlation with BUT (all P>0.05). Conclusion　In patients with dry eyes, the expression level of the Nod-1 in their tears is correlated with dry eye severity. The Nod-1, NF-κB p65, and IL-1β are all involved in the development of dry eyes. What’s more, Nod-1 expression may play an important role in causing inflammation in dry eyes.

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更新日期/Last Update: 2022-10-05