[1]司长峰,卜荔,徐楠,等.柴胡皂苷D对糖尿病视网膜病变大鼠的治疗作用[J].眼科新进展,2021,41(10):925-929.[doi:10.13389/j.cnki.rao.2021.0194]
　SI Changfeng,BU Li,XU Nan,et al.Therapeutical effect of Saikosaponin D on diabetic retinopathy of rats[J].Recent Advances in Ophthalmology,2021,41(10):925-929.[doi:10.13389/j.cnki.rao.2021.0194]
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
41卷
期数:
2021年10期
页码:
925-929
栏目:
实验研究
出版日期:
2021-10-05

文章信息/Info

Title:
Therapeutical effect of Saikosaponin D on diabetic retinopathy of rats
作者:
司长峰; 卜荔; 徐楠; 项燕; 顾永昊
239000　安徽省滁州市，滁州市第一人民医院眼科（司长峰，卜荔，徐楠，项燕）；230031　安徽省合肥市，安徽省立医院眼科（顾永昊）
Author(s):
SI Changfeng1; BU Li1; XU Nan1; XIANG Yan1; GU Yonghao2
1.Department of Ophthalmology,the First People’s Hospital of Chuzhou,Chuzhou 239000,Anhui Province,China
2.Department of Ophthalmology,Anhui Provincial Hospital,Hefei 230031,Anhui Province,China
关键词:
柴胡皂苷D; 糖尿病视网膜病变; 微血管渗透性; 炎症
Keywords:
saikosaponin D;  diabetic retinopathy;  microvascular permeability;  inflammation
分类号:
R774
DOI:
10.13389/j.cnki.rao.2021.0194
文献标志码:
A
摘要:
目的　探究柴胡皂苷D（SSD）对糖尿病视网膜病变（DR）大鼠视网膜的保护作用及其机制。方法　40只SD大鼠随机分为正常对照（Con）组、DR组、低剂量SSD组和高剂量SSD组，每组各10只。DR组、低剂量SSD组和高剂量SSD组通过高脂高糖饮食联合腹腔注射链脲佐菌素（STZ）构建糖尿病大鼠模型，然后对糖尿病大鼠持续喂养高脂高糖饮食12周构建DR大鼠模型，其中低剂量SSD组、高剂量SSD组大鼠在持续高脂高糖喂养期间分别给予口服1 mg·kg-1·d-1和5 mg·kg-1·d-1 SSD。Con组大鼠全程采用标准饲料喂养，且不给予STZ注射。在喂养方案结束时，异硫氰酸荧光素-葡聚糖（FITC-Dextran）染色评估视网膜微血管的渗透性；过碘酸雪夫（PAS）染色观察视网膜微血管病变程度；ELISA检测视网膜组织中肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-6和IL-1β的表达水平；Western blot检测视网膜组织中咬合蛋白（Occludin）、闭合蛋白5（Claudin-5）、闭锁小带蛋白1（ZO-1）、血管内皮生长因子α（VEGF-α）、胶质纤维酸性蛋白（GFAP）和血管内皮细胞黏附分子1（VCAM-1）的蛋白表达水平。结果　与Con组比较，DR组大鼠视网膜微血管的渗透性增加，视网膜毛细血管网中无细胞毛细血管（AC）和周细胞丢失（PL）的相对比例均增加，视网膜组织中TNF-α、IL-6、IL-1β、VEGF-α、GFAP和VCAM-1的表达水平均升高，而Occludin、Claudin-5和ZO-1的表达水平均降低（均为P<0.05）。与DR组比较，低剂量SSD组、高剂量SSD组大鼠视网膜微血管的渗透性均降低，视网膜毛细血管网中AC和PL的相对比例均降低，视网膜组织中TNF-α、IL-6、IL-1β、VEGF-α、GFAP和VCAM-1的表达水平均降低，而Occludin、Claudin-5和ZO-1表达水平均升高（均为P<0.05）；其中，高剂量SSD组大鼠上述指标均较低剂量SSD组大鼠变化更明显（均为P<0.05）。结论　SSD可能通过减轻视网膜微血管渗透性和炎症反应而发挥对DR大鼠视网膜的治疗作用。
Abstract:
Objective　To investigate the protective effect and mechanism of saikosaponin D (SSD) on the retina of diabetic retinopathy (DR) of rats.Methods　Forty SD rats were randomly divided into normal control (Con) group, DR group, low-dose SSD group and high-dose SSD group, with 10 rats each. DR group, low-dose SSD group and high-dose SSD group were fed with high-fat and high-sugar diet combined with intraperitoneal injection of streptozotocin (STZ) to construct diabetes mellitus (DM) rat model. The DM rats were fed with high-fat and high-sugar diet continuously for 12 weeks to build DR rat model; the low- and high-dose SSD groups were orally given 1 mg·kg-1·d-1 and 5 mg·kg-1·d-1 SSD during continuous high-fat and high-sugar feeding period, respectively. Con group was fed with standard diet throughout the whole process without STZ injection. At the end of the feeding period, the permeability of retinal microvessels was evaluated by FITC-Dextran; the retinal microvascular lesions were observed by periodic acid-schiff (PAS) staining. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-1β in the retina were detected by ELISA assay; the protein expression levels of Occludin, Claudin-5, zonula occludens protein-1 (ZO-1), vascular endothelial growth factor-α (VEGF-α), glial fibrillary acidic protein (GFAP) and vascular endothelial cell adhesion molecule-1 (VCAM-1) in the retina were detected by Western blot. Results　Compared with Con group, the permeability of retinal microvessels, the relative proportions of acellular capillaries (AC) and pericyte loss (PL), and the levels of TNF-α, IL-6, IL-1β, VEGF-α, GFAP and VCAM-1 in retina in DR group were increased, while the expression levels of Occludin, Claudin-5 and ZO-1 were decreased (all P<0.05). Compared with DR group, the permeability of retinal microvessels, the relative proportions of AC and PL, and the levels of TNF-α, IL-6, IL-1β, VEGF-α, GFAP and VCAM-1 in retina in low- and high-dose groups were decreased, while the expression levels of Occludin, Claudin-5 and ZO-1 were increased (all P<0.05). Among them, the changes in the above indicators in the high-dose SSD group were more significant than those in the low-dose SSD group (all P<0.05).Conclusion　SSD may play a protective role on the retina of DR rats by reducing the permeability and inflammation of retinal microvessels.

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备注/Memo

备注/Memo:
安徽省自然科学基金项目（编号：1908085MH254）
更新日期/Last Update: 2021-10-05