[1]杜红飞,庞雪利,阳燕,等.老年性白内障患者外周血环状RNA差异表达谱研究[J].眼科新进展,2020,40(9):849-852.[doi:10.13389/j.cnki.rao.2020.0193]
　DU Hongfei,PANG Xueli,YANG Yan,et al.Peripheral blood circRNAs expression profile in patients with senile cataract[J].Recent Advances in Ophthalmology,2020,40(9):849-852.[doi:10.13389/j.cnki.rao.2020.0193]
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老年性白内障患者外周血环状RNA差异表达谱研究/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
40卷
期数:
2020年9期
页码:
849-852
栏目:
应用研究
出版日期:
2020-09-05

文章信息/Info

Title:
Peripheral blood circRNAs expression profile in patients with senile cataract
作者:
杜红飞; 庞雪利; 阳燕; 周晓萍; 许颖
610500　四川省成都市，成都医学院第一附属医院
Author(s):
DU Hongfei; PANG Xueli; YANG Yan; ZHOU Xiaoping; XU Ying
The First Affiliated Hospital of Chengdu Medical College,Chengdu 610000,Sichuan Province,China
关键词:
老年性白内障; 生物信息学; 环状RNA; 表达谱
Keywords:
senile cataract;  bioinformatics;  circRNAs;  expression profile
分类号:
R776.1
DOI:
10.13389/j.cnki.rao.2020.0193
文献标志码:
A
摘要:
目的　利用微阵列芯片技术筛选老年性白内障患者外周血中差异性表达的环状 RNA(circular RNAs,circRNAs)进行生物信息学数据预测和分析，以探究外周血circRNAs在老年性白内障发病中的作用。方法　选取成都医学院第一附属医院已诊断为老年性白内障患者3例（试验组）和健康体检者3人（对照组）作为研究对象，运用Arraystar circRNAs基因芯片技术检测两组外周血中circRNAs表达谱及分析两组间差异表达的circRNAs。同时，运用在线生物信息学软件对差异circRNAs进行分析以及对下游相互作用的微小RNA（miRNA）进行预测。结果　与对照组比较，试验组外周血中异常表达的circRNAs共有242个(fold change绝对值≥2.0)，其中上调的100个(fold change≥2.0)，下调的142个 (fold change≤-2.0)；生物信息分析数据提示，差异circRNAs在调节细胞代谢、发育及RNA转录等功能方面高度富集；4个circRNAs与白内障相关的miR-15a-3p、miR-34a-5p等吸附相关miRNA可能具有miRNA海绵吸附作用。结论　试验组与对照组外周血之间存在差异表达的circRNAs，这些circRNAs可能参与老年性白内障的致病调控，未来可能成为老年性白内障新型诊断和治疗的潜在生物学标志物。
Abstract:
Objective　To screen the peripheral blood circRNAs differently expressed in patients with senile cataract and analyze the data with bioinformatics, which would provide the basis of research for pathogenic role of peripheral blood circRNAs in senile cataract.Methods　The 3 senile cataract patients who were diagnosed by the First Affiliated of Chengdu Medical College and 3 healthy controls were chosen to be subjects of study. The Arraystar circRNAs was performed to detect the expression profiles of circRNAs in peripheral blood of senile cataract patients and normal controls by Arraystar circRNAs gene chip technology. The difference analysis of expression profiles between groups was carried out to uncover different expressed circRNAs. Meanwhile, the bioinformatics analysis and adsorptions of circRNAs-related microRNAs (miRNA) were predicted.Results　In compassion with the normal controls, the peripheral blood of senile cataract patients had 242 significant altered circRNAs (absolute value of fold change≥2.0), containing 100 up-regulated (fold change≥2.0), and 142 down-regulated circRNAs (fold change≤-2.0). The results of bioinformatics analysis showed that these related to circRNA genes involved in regulating cell metabolism, development and RNA transcription. There were 4 circRNAs, which may sponge to cataract related microRNAs, including miR-15a-3p and miR-34a-5p-related.Conclusion　There are differently expressed circRNAs between peripheral blood of senile cataract patients and normal controls, which may involve in the regulatory pathogenesis mechanisms of senile cataract and be candidate biomarkers to diagnose and treat senile cataract in the future.

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备注/Memo:
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更新日期/Last Update: 2020-09-05