[1]隋文君,高洪莲,刘奇奇,等.PPAR-γ受体激动剂罗格列酮对兔准分子激光角膜切削术后角膜上皮下雾状混浊的影响[J].眼科新进展,2019,39(8):732-736.[doi:10.13389/j.cnki.rao.2019.0167]
　SUI Wen-Jun,GAO Hong-Lian,LIU Qi-Qi,et al.Effects of peroxisome proliferator-activated receptor gamma agonist rosiglitazone on corneal haze after photorefractive keratectomy in rabbits[J].Recent Advances in Ophthalmology,2019,39(8):732-736.[doi:10.13389/j.cnki.rao.2019.0167]
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PPAR-γ受体激动剂罗格列酮对兔准分子激光角膜切削术后角膜上皮下雾状混浊的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
39卷
期数:
2019年8期
页码:
732-736
栏目:
实验研究
出版日期:
2019-08-05

文章信息/Info

Title:
Effects of peroxisome proliferator-activated receptor gamma agonist rosiglitazone on corneal haze after photorefractive keratectomy in rabbits
作者:
隋文君; 高洪莲; 刘奇奇; 张磊
256600　山东省滨州市，滨州医学院附属医院眼科［隋文君（2016级硕士研究生），刘奇奇（2017级硕士研究生），张磊］；256600　山东省滨州市，滨州医学院附属医院临床医学实验室（高洪莲）
Author(s):
SUI Wen-Jun; GAO Hong-Lian; LIU Qi-Qi; ZHANG Lei
Department of Ophthalmology,the Affiliated Hospital of Binzhou Medical University （SUI Wen-Jun，LIU Qi-Qi，ZHANG Lei），Binzhou 256600，Shandong Province，China；Clinical Medical Laboratory of Affiliated Hospital of Binzhou Medical University （GAO Hong-Lian），Binzhou 256600，Shandong Province，China
关键词:
准分子激光角膜切削术; 角膜上皮下雾状混浊; 过氧化物酶体增殖物激活受体激动剂; 转化生长因子-β1; α-平滑肌肌动蛋白; 基质金属蛋白酶-2; Smad通路
Keywords:
photorefractive keratectomy; corneal haze; peroxisome proliferator-activated receptor agonist; transforming growth factors beta 1; alpha smooth muscle actin; matrix metalloproteinase -2; Smad pathway
分类号:
R778
DOI:
10.13389/j.cnki.rao.2019.0167
文献标志码:
A
摘要:
目的　探讨过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activated receptor gamma，PPARγ）激动剂罗格列酮对兔准分子激光角膜切削术（photorefractive keratectomy，PRK）术后角膜上皮下雾状混浊（haze）的影响。方法　选取普通级新西兰大白兔64只（64眼），随机分为28 μmol·L-1罗格列酮组、14 μmol·L-1罗格列酮组、1 g·L-1二甲基亚砜（dimethyl sulfoxide，DMSO）+生理盐水组、单纯PRK组，每组16只（16眼）。均行右眼PRK手术，术后分别给予28 μmol·L-1罗格列酮、14 μmol·L-1罗格列酮、1 g·L-1DMSO+生理盐水滴眼，单纯PRK组未作任何特殊处理。术后各组用裂隙灯观察并比较角膜上皮愈合情况；分别于PRK术后7 d、28 d比较各组haze情况并行眼前节照相；每组分别于PRK术后7 d、28 d分批处死8只兔，取角膜行免疫组织化学法比较各组转化生长因子-β1（transforming growth factor-β1，TGF-β1）、α-平滑肌肌动蛋白（α-smooth muscle actin，α-SMA）、基质金属蛋白酶-2（matrix metalloproteinases-2，MMP-2）的表达。结果　PRK术后角膜上皮愈合时间组间差异无统计学意义（P＞0.05）；PRK术后7 d、28 d，各组haze分级及TGF-β1、α-SMA、MMP-2的表达情况，组间差异均有统计学意义（均为P＜0.05），其中28 μmol·L-1罗格列酮组haze最轻，14 μmol·L-1罗格列酮组其次，明显轻于1 g·L-1 DMSO+生理盐水组及单纯PRK组，除1 g·L-1DMSO+生理盐水组及单纯PRK组组间差异均无统计学意义（均为P＞0.05）外，其余组间两两比较差异均有统计学意义（均为P＜0.05）。结论　PPAR-γ受体激动剂罗格列酮对兔PRK术后haze有抑制作用，呈浓度依赖性，可能由TGF-β1/Smad通路介导。
Abstract:
Objective　To investigate the effects of peroxisome proliferator-activated receptor gamma (PPAR-γ) agonist rosiglitazone on corneal haze after photorefractive keratectomy (PRK) in rabbits.Methods　Totally 64 New Zealand white rabbits were selected and randomly divided into four groups,28 μmol·L-1 rosiglitazone group,14 μmol·L-1 rosiglitazone group,1 g·L-1 dimethyl sulfoxide(DMSO)+normal saline (NS) group and PRK group,sixteen rabbits(16 eyes) in each group.PRK was performed on the right eye in all groups.28 μmol·L-1 rosiglitazone group,14 μmol·L-1 rosiglitazone group and 1 g·L-1 DMSO+NS group was given 28 μmol·L-1 rosiglitazone,14 μmol·L-1 rosiglitazone and 1 g·L-1 DMSO+NS eye drops，respectively,no intervention was performed in the PRK group.Slit lamp was used to observe and compare the corneal epithelium healing in each group.On the 7th day and 28th day after PRK,haze was compared and recorded by the anterior segment photographs.Eight rabbits were sacrificed in each group,all of the corneas were obtained and then immunohistochemistry was performed to detect the expressions of transforming growth factors beta 1(TGF-β1),alpha smooth muscle actin(α-SMA) and matrix metalloproteinase-2 (MMP-2).Results　There was no significant difference in healing time of corneal epithelium after PRK in each group(P＞0.05).On the 7th day and 28th day after PRK,there were statistically significant differences in haze grade and the expressions of TGF-β1,α-SMA and MMP-2 in each group(both P＜0.05).Haze classification and expressions of TGF-β1,α-SMA and MMP-2 in 28 μmol·L-1 rosiglitazone group were both the most light,followed by 14 μmol·L-1 rosiglitazone group,which significantly lighter than that in 1 g·L-1 DMSO+NS group and PRK group.Except that there was no statistical difference between 1 g·L-1 DMSO+NS group and PRK group(all P＞0.05),and pairwise comparison was statistically significant between the other groups(all P＜0.05).Conclusion　PPAR-γ agonist rosiglitazone can inhibit corneal haze after PRK in rabbits,the effect of high concentration is more obvious than that of low concentration,which maybe mediated by the TGF-β1/Smad pathway.

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相似文献/References:

[1]彭志优 周琼 邵毅 刘永琰 李汉林.兔LASIK术后角膜瓣弃留与紫外线照射对角膜上皮下雾状混浊影响的实验研究[J].眼科新进展,2012,32(11):000.
[2]郑磊,雷蕾,张建华,等.PRK与LASIK术后角膜组织神经肽P物质表达的研究[J].眼科新进展,2010,30(5):000.

备注/Memo

备注/Memo:
山东省医药卫生科技发展计划项目（编号：2016WS0022）
更新日期/Last Update: 2019-07-31