[1]吴德福,周利晓,吕梁,等.我国原发性青光眼患者外周血中含缬酪肽蛋白和α-胞衬蛋白含量的研究[J].眼科新进展,2018,38(10):968-971.[doi:10.13389/j.cnki.rao.2018.0229]
 WU De-Fu,ZHOU Li-Xiao,LV Liang,et al.The levels of valosin-containing protein and alpha-fodrin in peripheral blood of patients with primary glaucoma in China[J].Recent Advances in Ophthalmology,2018,38(10):968-971.[doi:10.13389/j.cnki.rao.2018.0229]
点击复制

我国原发性青光眼患者外周血中含缬酪肽蛋白和α-胞衬蛋白含量的研究/HTML
分享到:

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年10期
页码:
968-971
栏目:
应用研究
出版日期:
2018-10-05

文章信息/Info

Title:
The levels of valosin-containing protein and alpha-fodrin in peripheral blood of patients with primary glaucoma in China
作者:
吴德福周利晓吕梁许冬董敬民
450052 河南省郑州市,郑州大学第五附属医院眼科(吴德福,周利晓,吕梁,许冬);450052 河南省郑州市,郑州大学第一附属医院眼科(董敬民)
Author(s):
WU De-FuZHOU Li-XiaoLV LiangXU DongDONG Jing-Min
Department of Ophthalmology,the Fifth Affiliated Hospital of Zhengzhou University(WU De-Fu,ZHOU Li-Xiao,LV Liang,XU Dong),Zhengzhou 450052,Henan Province,China;The First Affiliated Hospital of Zhengzhou University(DONG Jing-Min),Zhengzhou 450052,Henan Province,China
关键词:
青光眼免疫因素含缬酪肽蛋白α-胞衬蛋白
Keywords:
glaucomaimmune factorsvalosin-containing proteinα-fodrin
分类号:
R775.1
DOI:
10.13389/j.cnki.rao.2018.0229
文献标志码:
A
摘要:
目的 检测不同类型青光眼患者与正常人外周血中含缬酪肽蛋白(valosin-containing protein,VCP)、α-胞衬蛋白(α-fodrin)的水平差异,评估各指标在青光眼诊断中的价值。方法 收集2015年1月至2017年9月首诊于我院和郑州大学第一附属医院眼科的青光眼患者80例,其中原发性闭角型青光眼(primary angle-closure glaucoma,PACG)组40例,原发性开角型青光眼(primary open angle glaucoma,POAG) 组40例;同期收集我院健康体检者40例作为对照组。采用ELISA法分别测定各组外周血中VCP和α-fodrin的含量,并作对比,同时对上述指标绘制受试者工作特征曲线,计算曲线下面积。采用多因素Logistic回归分析青光眼患者发病的危险因素。结果 POAG组、PACG组和对照组VCP含量分别为(124.866±34.858)ng·L-1、(94.143±28.970)ng·L-1和(44.707±8.892)ng·L-1,α-fodrin含量分别为(0.531±0.306)μg·L-1、(0.399±0.213)μg·L-1、(0.304±0.214)μg·L-1,3组间两两相比,VCP、α-fodrin含量的差异均有统计学意义(均为P<0.05)。受试者工作特征曲线下面积分析结果显示,VCP为0.754(95%CI为 0.646~0.862,P<0.001),α-fodrin为0.610(95%CI为0.486~0.733,P=0.091)。多因素Logistic回归分析提示,仅VCP是POAG患者发病的独立危险因素(OR=9.134,P<0.01)。结论 POAG患者外周血中VCP和α-fodrin的含量高于PACG患者和正常人群,提示免疫因素可能在青光眼视神经损害机制中起重要作用,VCP是POAG发病的独立危险因素。
Abstract:
Objective To detect the difference in valosin-containing protein (VCP) and α-fodrin contents in peripheral blood of patients with different types of glaucoma and normal people,and to evaluate the value of each index in the diagnosis of glaucoma.Methods Eighty patients with glaucoma for first diagnosis in our department and the Ophthalmology of the First Affiliated Hospital of Zhengzhou University from January 2015 to September 2017 were enrolled,including primary angle-closure glaucoma (PACG) group (40 patients) and primary open angle glaucoma (POAG) group (40 patients).Another 40 patients of healthy physical examination in our hospital were collected as control group during the same period.The contents of VCP and α-fodrin in peripheral blood of each group were determined by enzyme-linked immunosorbent assay (ELISA),followed by the comparison of the indicators.The receiver operating characteristic curve of the subjects were plotted.The area under the curve was calculated.Multivariate logistic regression was used to analyze the risk factors of glaucoma patients.Results The VCP content was (124.866±34.858)ng·L-1,(94.143±28.970)ng·L-1,(44.707±8.892)ng·L-1,while α-fodrin concentration was(0.531±0.306)μg·L-1,(0.399±0.213)μg·L-1,(0.304±0.214)μg·L-1 in the POAG group,PACG group and control group,respectively.There were significant differences in the content of VCP and α-fodrin between the POAG group,PACG group and control group in pairwise comparison (all P<0.05).The area under the receiver operating characteristic curve analysis showed that the VCP was 0.754 (95%CI 0.646-0.862,P<0.001) and α-fodrin was 0.61 (95%CI 0.486-0.733,P=0.091).Multivariate logistic regression analysis indicated that only VCP was an independent risk factor for the pathogenesis of POAG (OR=9.134,P<0.01).Conclusion The contents of VCP and α-fodrin in peripheral blood of patients with POAG are higher than those of patients with PACG and normal controls.It suggests that immune factors may play an important role in the mechanism of glaucomatous optic nerve damage.VCP is an independent risk factor for the pathogenesis of POAG.

参考文献/References:

[1] WEINREB R N,KHAW P T.Primary open-angle glaucoma[J].Lancet,2004,363(9422):1711-1720.
[2] LIU Y Y,YU H.Effect of compound trabeculectomy on acute primary angle closure glaucoma under continuous high intraocular pressure state[J].J Xingxiang Med Coll,2015,32(2):160-162.
刘艳艳,余涵.原发性急性闭角型青光眼患者持续高眼压状态下行复合式小梁切除术疗效分析[J].新乡医学院学报,2015,32(2):160-162.
[3] LI X P,GAO J W,WANG S,LI Y,LIU J.Clinical effect of phacoemulsification combined with intraocular lens implantation in management of primary angle-closure glaucoma with cataract[J].J Xingxiang Med Coll,2015,32(2):169-170,174.
李晓鹏,高建伟,王爽,李彦,刘静.白内障超声乳化联合人工晶体植入术治疗 原发性青光眼合并白内障临床疗效观[J].新乡医学院学报,2015,32(2):169-170,174.
[4] HUANG P,ZHANG C.IL-4,IL-6 and IL-12 levels in patients with open-angle glaucoma[J].Rec Adv Ophthalmol,2005,25(1):41-42.
黄萍,张纯.开角型青光眼患者外周血中IL-4、IL-6、IL-12含量的研究[J].眼科新进展,2005,25(1):41-42.
[5] TEZEL G,WAX M B.The immune system and glaucoma[J].Curr Opin Ophthalmol,2004,15(2):80-84.
[6] WAX M B,YANG J,TEZEL G.Serum autoantibodies in patients with glaucoma[J].J Glaucoma,2001,10(5 Suppl):S22-24.
[7] WAX M,YANG J,TEZEL G.Autoantibodies in glaucoma[J].Curr Eye Res,2002,25(2):113-116.
[8] GRUS F H,JOACHIM S C,WUENSCHIG D,RIECK J,PFEIFFER N.Autoimmunity and glaucoma[J].J Glaucoma,2008,17(1):79-84.
[9] JONAS J B,GEORGE R,ASOKAN R,FLAXMAN S R,KEEFFE J,LEASHER J,et al.Prevalence and causes of vision loss in Central and South Asia:1990-2010[J].Br J Ophthalmol,2014,98(5):592-598.
[10] GE J,HE G M,ZHAO J L,FANG M,ELLWEIN L B,HE N,et al.Prevalence of blindness and moderate and severe visual impairment among adults aged 50 years or above in Yangxi County of Guangdong Province:the China Nine-Province Survey[J].Chin J Ophthalmol,2014,50(3):167-172.
葛坚,何光明,赵家良,方敏,ELLWEIN L B,何宁,等.我国九省眼病调查中广东省阳西县50岁及以上人群盲和中、重度视力损伤患病率及致病原因调查[J].中华眼科杂志,2014,50(3):167-172.
[11] LI W Y,HUANG P.Immunization and glaucoma[J].Chin J Exp Ophthalmol,2014,32(5):466-470.
李维义,黄萍.免疫与青光眼[J].中华实验眼科杂志,2014,32(5):466-470.
[12] BAKALASH S,KIPNIS J,YOLES E,SCHWARTZ M.Resistance of retinal ganglion cells to an increase in intraocular pressure is immune-dependent[J].Invest Ophthalmol Vis Sci,2002,43(8):2648-2653.
[13] HATA M,IKEDA H O,KIKKAWA C,IWAI S,MURAOKA Y,HASEQAWA T,et al.KUS121,a VCP modulator,attenuates ischemic retinal cell death via suppressing endoplasmic reticulum stress[J].Sci Rep,2017,7:44873.
[14] LEE K J,JEONG S M,HOEHN B D,HONG Y J,LEE S H.Valosin-containing protein is a novel autoantigen in patients with glaucoma[J].Optom Vis Sci,2011,88(1):164-172.
[15] PYE V E,DREVENY I,BRIGGS L C,SANDS C,BEURON F,ZHANG X,et al.Going through the motions:the ATPase cycle of p97[J].J Struct Biol,2006,156(1):12-28.
[16] BARBIN L,EISELE F,SANTT O,WOLF D H.The Cdc48-Ufd1-Npl4 complex is central in ubiquitin-proteasome triggered catabolite degradation of fructose-1,6-bisphosphatase[J].Biochem Biophys Res Commun,2010,394(2):335-341.
[17] DEICHSEL A,MOUYSSET J,HOPPE T.The ubiquitin-selective chaperone CDC-48/p97,a new player in DNA replication[J].Cell Cycle,2009,8(2):185-190.
[18] MEYER H,POPP O.Role(s) of Cdc48/p97 in mitosis[J].Biochem Soc Trans,2008,36(1):126-130.
[19] RAASI S,WOLF D H.Ubiquitin receptors and ERAD:a network of pathways to the proteasome[J].Semin Cell Dev Biol,2007,18(6):780-791.
[20] DAI R M,LI C C.Valosin-containing protein is a multi-ubiquitin chain-targeting factor required in ubiquitin-proteasome degradation[J].Nat Cell Biol,2001,3(8):740-744.
[21] EGERTON M,ASHE O R,CHEN D,DRUKER B J,BURGESS W H,SAMELSON L E.VCP,the mammalian homolog of cdc48,is tyrosine phosphorylated in response to T cell antigen receptor activation[J].EMBO J,1992,11(10):3533-3540.
[22] BRAUN R J,ZISCHKA H.Mechanisms of Cdc48/VCP-mediated cell death:from yeast apoptosis to human disease[J].Biochim Biophys Acta,2008,1783(7):1418-1435.
[23] TEZEL G,SEIGEL G M,WAX M B.Autoantibodies to small heat shock proteins in glaucoma[J].Invest Ophthalmol Vis Sci,1998,39(12):2277-2287.
[24] WANG Y,VIRJI A S,HOWARD P,SAYANI Y,ZHANG J,ACHU P,et al.Detection of cleaved alpha-fodrin autoantigen in Sjgren’s syndrome:apoptosis and co-localisation of cleaved alpha-fodrin with activated caspase-3 and cleaved poly(ADP-ribose) polymerase (PARP) in labial salivary glands[J].Arch Oral Biol,2006,51(7):558-566.
[25] TAHZIB N G,RANSOM N L,REITSAMER H A,MCKINNON S J.Alpha-fodrin is cleaved by caspase-3 in a chronic ocular hypertensive (COH) rat model of glaucoma[J].Brain Res Bull,2004,62(6):491-495.
[26] ROHN T T,RISSMAN R A,DAVIS M C,KIM Y E,COTMAN C W,HEAD E.Caspase-9 activation and caspase cleavage of tau in the Alzheimer’s disease brain[J].Neurobiol Dis,2002,11(2):341-354.
[27] GRUS F H,JOACJIM S C,BRUNS K,LACKNER K J,PFEIFFER N,WAX M B.Serum autoantibodies to alpha-fodrin are present in glaucoma patients from Germany and the United States[J].Invest Ophthalmol Vis Sci,2006,47(3):968-976.
[28] MCIIWAIN D R,BERGER T,MAK T W.Caspase functions in cell death and disease[J].Cold Spring Harb Perspect Biol,2013,5(4):a008656.
[29] NAKATSUMI H,YONEHARA S.Identification of functional regions defining different activity in caspase-3 and caspase-7 within cells[J].J Biol Chem,2010,285(33):25418-25425.

相似文献/References:

[1]李翔 谢钊 郭红建 谢学军 路雪婧 王毅 王超.补肾活血中药对大鼠慢性高眼压模型外侧膝状体病理改变的影响[J].眼科新进展,2012,32(1):000.
[2]范虹 刘五存 蔡鸿英 赵堪兴.改良二极管激光睫状体光凝术治疗中晚期青光眼[J].眼科新进展,2012,32(4):000.
[3]王建萍 赵燕麟 马勇 朱涛 程燕 车选义 赵桂娥 王柯.噻吗洛尔和布林佐胺联合曲伏前列素治疗原发性开角型青光眼与高眼压患者的临床研究[J].眼科新进展,2012,32(5):000.
[4]彭坤 靳隽 杨玉新 许银霞 王保君 闫义涛 杨华.长期联合应用噻吗洛尔对结膜组织炎性标记物ICAM-1和HLA-DR表达的影响[J].眼科新进展,2012,32(6):000.
[5]马恩普 赵小钊 董良 刘苏冰 曾照年.Healaflow在青光眼小梁切除术中的应用[J].眼科新进展,2012,32(6):000.
[6]王勇 叶应嘉 鲍先议 周龑丽 许荣 彭婷婷 曾志富.同轴微小切口超声乳化吸出术在青光眼滤过术后白内障摘出术中的应用[J].眼科新进展,2012,32(7):000.
[7]李翔 马世勇 李娟 王毅.补肾活血中药对大鼠慢性高眼压模型视神经病理改变的影响[J].眼科新进展,2013,33(2):000.
[8]白东娥 刘伟 季建.抗青光眼药物对青光眼患者泪液胰岛素水平的影响及其与眼表改变的关系[J].眼科新进展,2013,33(6):000.
[9]马英慧 张铁民 齐建平.原发性开角型青光眼与慢性原发性闭角型青光眼视网膜神经纤维层厚度与视野缺损的关系[J].眼科新进展,2013,33(7):000.
[10]张海涛 杨玉新 毛永 丁晓丽 秦海霞 梁长华 郭英昌.青光眼与非炎症性缺血型视神经病变的傅立叶OCT扫描视神经形态学对比[J].眼科新进展,2013,33(8):000.

备注/Memo

备注/Memo:
河南省医学科技攻关项目(编号:201503131)
更新日期/Last Update: 2018-09-28