[1]梁丽娜,李雪丽,许凯,等.补肾益精方对先天性视网膜色素变性RCS大鼠感光细胞凋亡的抑制作用[J].眼科新进展,2018,38(7):611-615.[doi:10.13389/j.cnki.rao.2018.0144]
 LIANG Li-Na,LI Xue-Li,XU Kai,et al.Effects of Bu Shen Yi Jing Fang on photoreceptor cell apoptosis in RCS rat with inherited retinal degeneration[J].Recent Advances in Ophthalmology,2018,38(7):611-615.[doi:10.13389/j.cnki.rao.2018.0144]
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补肾益精方对先天性视网膜色素变性RCS大鼠感光细胞凋亡的抑制作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年7期
页码:
611-615
栏目:
实验研究
出版日期:
2018-07-05

文章信息/Info

Title:
Effects of Bu Shen Yi Jing Fang on photoreceptor cell apoptosis in RCS rat with inherited retinal degeneration
作者:
梁丽娜李雪丽许凯陈强张晶梁洁唐由之
100040 北京市,中国中医科学院眼科医院
Author(s):
LIANG Li-NaLI Xue-LiXU KaiCHEN QiangZHANG JingLIANG JieTANG You-Zhi
Eye Hospital,China Academy of Chinese Medical Sciences,Beijing 100040,China
关键词:
补肾益精方视网膜色素变性感光细胞凋亡神经营养因子RCS大鼠
Keywords:
Bu Shen Yi Jing Fangretinitis pigmentosaphotoreceptor cellapoptosisneurotrophic factorRCS rat
分类号:
R774
DOI:
10.13389/j.cnki.rao.2018.0144
文献标志码:
A
摘要:
目的 探讨补肾益精方对先天性视网膜色素变性RCS大鼠感光细胞凋亡的影响。方法 将先天性视网膜色素变性模型RCS大鼠24只随机分为补肾益精方组及蒸馏水组,分别给予补肾益精方药液及蒸馏水灌胃,12只健康SD大鼠常规饲养作为正常组。在给药7 d及28 d后HE染色观察各组大鼠视网膜组织病理学变化,TUNEL法检测细胞凋亡情况,实时荧光定量PCR检测视网膜中睫状神经营养因子、脑源性神经营养因子以及碱性成纤维细胞生长因子表达情况。结果 HE染色切片光学显微镜下正常组SD大鼠视网膜结构清晰,层次分明,各层细胞排列整齐。蒸馏水组大鼠视网膜内核层及外核层均较正常组明显变薄,细胞排列稀疏,可见空泡样改变,感光细胞数减少,且随鼠龄增加减少日益显著。补肾益精方组大鼠视网膜内核层及外核层亦较正常组变薄,但与蒸馏水组相比增厚,感光细胞数较后者增多,细胞排列也较为整齐。给药7 d及28 d时补肾益精方组每个高倍视野感光细胞数分别为140±9和80±9,蒸馏水组分别为113±8和44±6,补肾益精方组较蒸馏水组明显增多,差异有统计学意义(均为P<0.05)。TUNEL检测结果显示给药7 d及28 d时补肾益精方组感光细胞凋亡率分别为31.67±5.39%及29.68±4.31%,蒸馏水组分别为50.34±5.21%及44.02±7.17%,补肾益精方组较蒸馏水组均明显降低,差异均有统计学意义(均为P<0.05)。实时荧光定量PCR结果显示给药7 d及28 d补肾益精方组视网膜睫状神经营养因子表达均较蒸馏水组增加(均为P<0.05),给药7 d时补肾益精方组视网膜脑源性神经营养因子表达较蒸馏水组明显增加(P<0.05),28 d时两组差异无统计学意义(P>0.05);给药7 d及28 d两组碱性成纤维细胞生长因子的表达差异均无统计学意义(均为P>0.05)。结论 补肾益精方对RCS大鼠感光细胞凋亡有明显的抑制作用,其作用机制可能与补肾益精方促进视网膜分泌神经营养因子有关。
Abstract:
Objective To observe the effects of Bu Shen Yi Jing Fang on photoreceptor cell apoptosis in royal college of surgeons rat (RCS) with inherited retinal degeneration and investigate the mechanisms.Methods Totally 24 RCS rats were randomly divided into Bu Shen Yi Jing Fang group and distilled water group.The rats were gavaged with either Bu Shen Yi Jing Fang solution or distilled water every day.And 12 healthy SD rats were fed regularly as the normal control.On day 7,day 28 after treatment,HE staining and TdT-mediated dUTP nick end labeling (TUNEL) method were used to detect retina histopathological changes and cell apoptosis rate.Quantitative real time polymerase chain reaction (q-PCR) was used to determine the effects of Bu Shen Yi Jing Fang on the expression of ciliary neurotrophic factor (CNTF),brain derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF).Results The slides processed with HE staining were observed under light microscope.The retina structure in normal SD rats was clear and well arranged,and the cells in each layer were in good order.The inner nuclear layer and outer nuclear layer in distilled water group were markedly thinner than those in the normal group.Cells arrange sparsely and vacuoles change could be observed in the cells.The number of photoreceptor cells decreased,which aggravated with the rat age.The inner nuclear layer and outer nuclear layer in Bu Shen Yi Jing Fang group were thinner than those in the normal group too,but thicker when compared with that of distilled water group.The number of photoreceptor cell was more than the later,and arranged more orderly.On day 7 and day 28 after treatment,the number of photoreceptor cells in Bu Shen Yi Jing Fang group were 140±9 and 80±9,while its number in distilled water group was 113±8 and 44±6 respectively.The photoreceptor number in Bu Shen Yi Jing Fang group was significantly more than that of the distilled water group (both P<0.05).It was shown by TUNEL detection that the apoptosis rates of photoreceptor cell in Bu Shen Yi Jing Fang group were (31.67±5.39)% and (29.68±4.31)% on day 7 and day 28 after treatment,while the apoptosis rates in distilled water group were (50.34±5.21)% and (44.02±7.17)% respectively.The apoptosis rates in Bu Shen Yi Jing Fang group were significantly lower than those of distilled water group (both P<0.05).It was shown by q-PCR detection that expression of CNTF in Bu Shen Yi Jing Fang group was significantly higher than that of distilled water group both on day 7 and day 28 after treatment (both P<0.05).On day 7 after treatment,expression of BDNF in Bu Shen Yi Jing Fang group was significantly higher than that of distilled water group (P<0.05).However,there was no significant difference between the two groups on day 28 after treatment (P>0.05).And the difference of bFGF expression between the two groups was not significantly different both on day 7 and day 28 after treatment (both P>0.05).Conclusion Bu Shen Yi Jing Fang can protect the apoptosis of photoreceptor cells in RCS rats significantly,and the mechanism may be related to the promotion of retinal neurotrophic factor expression.

参考文献/References:

[1] DIAS M F,JOO K,KEMP J A,FIALHO S L,DA SILVA CUNHA A J R,WOO S J,et al.Molecular genetics and emerging therapies for retinitis pigmentosa:Basic research and clinical perspectives[J].Prog Retin Eye Res,2017,63:107-131.
[2] TANG Z,ZHANG Y,WANG Y,ZHANG D,SHEN B,LUO M,et al.Progress of stem/progenitor cell-based therapy for retinal degeneration[J].J Transl Med,2017,15(1):99.
[3] XU J,PENG Q.Retinitis pigmentosa treatment with western medicine and traditional chinese medicine therapies[J].J Ophthalmol,2015,2015:421269.
[4] LI X L,TANG Y Z,FAN J P,XU K,LIANG J,LIANG L N.Study of Bushenyijing Decoction’s protective effect on retinal degeneration in RCS rat[J].J Tradit Chin Ophthalmol,2016,26(3):144-149.
李雪丽,唐由之,范吉平,许凯,梁洁,梁丽娜.补肾益精方对RCS大鼠视网膜变性损伤的保护作用研究[J].中国中医眼科杂志,2016,26(3):144-149.
[5] D’CRUZ P M,YASUMURA D,WEIR J,MATTHES M T,ABDERRAHIM H,LAVAIL M M,et al.Mutation of the receptor tyrosine kinase gene (Mertk) in the retinal dystrophic RCS rat[J].Hum Mol Genet,2000,9(4):645-651.
[6] LIU B,TANG J M,ZHU X A,TANG Y.Photoreceptor apoptosis in hereditary degenerative retina of RCS rats[J].Acta Anat Sin,1998,29(4):410-412.
刘斌,唐军民,朱秀安,唐岩.RCS大鼠视网膜感光细胞的凋亡[J].解剖学报,1998,29(4):410-412.
[7] GUADAGNI V,NOVELLI E,PIANO I,GARGINI C,STRETTOI E.Pharmacological approaches to retinitis pigmentosa:A laboratory perspective[J].Prog Retin Eye Res,2015,48:62-81.
[8] ZHOU L,YANG Q D,YUAN M S,MA Z J,HOU D R,TAN X L.Effect of tuber fleeceflower root on disorders of learning and memory and neuronal apoptosis in hippocampi induced by beta-amyloid [J].Chin J Tis Engineer Res,2005,9(9):131-134.
周琳,杨期东,袁梦石,马志健,侯德仁,谭兴林.何首乌对淀粉样β蛋白致海马神经元的凋亡和学习记忆障碍的作用[J].中国组织工程研究,2005,9(9):131-134.
[9] WEN Z,XIAO Y S,TANG N,WU D F,ZHANG J,NIE R Q,et al.Effect of polygonum multiflorum on apoptosis and learning and memory impairment induced by amyloid beta protein in hippocampal neurons [J].Pharm Clin Chin Mater Med,2006,22(2):29-31.
文珠,肖移生,唐宁,吴东风,张进,聂荣庆,等.黄精多糖对神经细胞的毒性及抗缺氧性坏死和凋亡作用研究[J].中药药理与临床,2006,22(2):29-31.
[10] WANG W H,LIU X H,CHI H F.Neuroprotective effects of Lycium barbarum on the retina of monocular visual deprived amblyopia in rats[J].Chin J Neuroanat,2010,26(3):268-272.
[11] LIU X H,NI T S,CHI H F.Neuroprotective effects of lycium barbarum on the Hereditary retinal dystrophy of RCS rats[J].Chin J Neuroanat,2008,24(6):602-606.
[12] KOLOMEYER A M,ZARBIN M A.Trophic factors in the pathogenesis and therapy for retinal degenerative diseases[J].Surv Ophthalmol,2014,59(2):134-165.
[13] DUTT K,CAO Y,EZEONU I.Ciliary neurotrophic factor:a survival and differentiation inducer in human retinal progenitors[J].In Vitro Cell Dev Biol Anim,2010,46(7):635-646.
[14] LIPINSKI D M,BARNARD A R,SINGH M S,MARTIN C,LEE E J,DAVIES W I L,et al.CNTF gene therapy confers lifelong neuroprotection in a mouse model of human retinitis pigmentosa[J].Mol Ther,2015,23(8):1308-1319.
[15] YAN B J,LI G L.Current advances on the brain-derived neurotrophic factor in therapy of retinitis pigmentosa [J].Chin J Exp Ophthalmol,2017,35(3):267-271.
闫博婧,李根林.脑源性神经营养因子治疗视网膜色素变性的研究进展[J].中华实验眼科杂志,2017,35(3):267-271.
[16] CHEN R,YIN X B,PENG C X,LI G L.Effect of brain-derived neurotrophic factor on c-jun expression in the rd mouse retina[J].Int J Ophthalmol,2012,5(3):266-271.

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备注/Memo

备注/Memo:
国家自然科学基金资助(编号:81473736、81674033、81102618);中国中医科学院中医药“一带一路”合作专项(编号:GH2017-04-02);国家人力资源和社会保障部留学人员科技活动择优项目(编号:2010-94)
更新日期/Last Update: 2018-07-12