[1]毛英,白海霞,畅颖,等.星形胶质细胞活化基因-1（AEG-1）在葡萄膜黑色素瘤中的表达及其与临床组织病理学的关系[J].眼科新进展,2018,38(2):121-125.[doi:10.13389/j.cnki.rao.2018.0026]
　MAO Ying,BAI Hai-Xia,CHANG Ying,et al.Expression of astrocyte elevated gene-1 (AEG-1) in uveal melanoma and its relationship with clinical histopathology[J].Recent Advances in Ophthalmology,2018,38(2):121-125.[doi:10.13389/j.cnki.rao.2018.0026]
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星形胶质细胞活化基因-1（AEG-1）在葡萄膜黑色素瘤中的表达及其与临床组织病理学的关系/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年2期
页码:
121-125
栏目:
实验研究
出版日期:
2018-02-05

文章信息/Info

Title:
Expression of astrocyte elevated gene-1 (AEG-1) in uveal melanoma and its relationship with clinical histopathology
作者:
毛英; 白海霞; 畅颖; 高飞; 张志豹; 张旭; 李彬
100005　北京市，首都医科大学附属北京同仁医院，北京同仁眼科中心，北京市眼科研究所，眼科学与视觉科学北京市重点实验室
Author(s):
MAO Ying; BAI Hai-Xia; CHANG Ying; GAO Fei; ZHANG Zhi-Bao; ZHANG Xu; LI Bin
Beijing Institute of Ophthalmology，Beijing Tongren Eye Center，Beijing Tongren Hospital，Capital Medical University；Beijing Ophthalmology & Visual Sciences Key Laboratory，Beijing 100005，China
关键词:
葡萄膜黑色素瘤; 星形胶质细胞活化基因-1; 高危因素; 免疫组织化学
Keywords:
uveal melanoma; astrocyte elevated gene-1 protein; high-risk factors; immunohistochemistry
分类号:
R773.4
DOI:
10.13389/j.cnki.rao.2018.0026
文献标志码:
A
摘要:
目的　研究葡萄膜黑色素瘤（uveal melanoma，UM）组织中星形胶质细胞活化基因-1 （astrocyte elevated gene-1，AEG-1）的表达及其与临床组织病理学的关系。方法　收集北京同仁医院眼科2013年1月至2017年5月，经眼科病理室确诊为UM且临床资料完善的50例患者（50眼）UM石蜡组织标本为实验组，11例（11眼）因外伤行眼球摘除但大部分葡萄膜组织正常的石蜡组织标本为对照组。应用免疫组织化学法检测所有石蜡标本中AEG-1的表达，并分析UM临床病理学特征与AEG-1的相关性。结果　AEG-1蛋白染色呈粉红色或者红色，主要定位于UM核周、细胞浆和细胞膜。实验组中，AEG-1阴性表达21眼（42.0%），阳性表达29眼（58.0%）；对照组中AEG-1均呈阴性表达，两组AEG-1表达比较差异有显著统计学意义（P=0.000），提示AEG-1在UM中高表达。在实验组中，当UM患眼存在临床组织病理学高危因素时，即存在肿瘤累及睫状体、虹膜新生血管生成、肿瘤基底最大直径>16 mm、肿瘤高度>8 mm、肿瘤细胞为混合细胞型、肿瘤细胞属于上皮样或混合细胞型、肿瘤眼外生长的患者，其AEG-1阳性表达率分别为73.1%、73.9%、95.2%、82.4%、83.3%、74.1%、69.4%，明显高于非高危因素患者，以上各特征组AEG-1表达比较差异均有统计学意义（均为P<0.05）。而患者性别、眼别、年龄、累及视盘与否、继发视网膜脱离与否、上皮样细胞型、侵犯巩膜导管与否组AEG-1表达差异均无统计学意义（均为P>0.05）。结论　AEG-1在UM组织中高表达，且与UM临床组织病理学多种高危因素呈显著相关性，提示AEG-1的高表达有可能成为判断UM患者预后的重要指标之一。
Abstract:
Objective　To investigate the relationship between the expression of astrocyte elevated gene-1 (AEG-1) and its clinical pathological features in uveal melanoma.Methods　Paraffin tissue specimens of 50 patients (50 eyes) with confirmed diagnosis of uveal melanoma (UM) were collected as experimental group，and samples from 11 patients (11 eyes) who underwent ocular excision due to trauma，still with largely intact uveal tissue，were selected as control group in the Department of Ophthalmology of Beijing Tongren Hospital from January 2013 to May 2017.Then the expression of AEG-1 in paraffin-embedded specimens was detected by immunohistochemical methods，and the correlation of AEG-1 expression with the clinical pathological data was analyzed.Results　AEG-1 protein staining presented accurate pink or red granules，mainly located in UM nucleus，cytoplasm，cell membrane.In the experimental group，negative expression of AEG-1 was in 21 eyes (42.0%)，and positive expression was in 29 eyes (58.0%)，while AEG-1 were negatively expressed in the control group，and the difference in the two groups was statistically significant (P=0.000)，suggesting that the expression of AEG-1 was upregulated in UM.In the experimental group，AEG-1 positive expression rate in patients with clinical pathological risk factors was significantly higher than that in patients without high risk factors (all P<0.05)，and the positive expression rate of AEG-1 in UM with involvement of the ciliary body and iris neovascularization，the maximum diameter of tumor base>16 mm，tumor thickness>8 mm，tumor cell type belonging to mixed cell type and epithelioid/mixed cell type，and extra ocular tumor growth was 73.1%，73.9%，95.2%，82.4%，83.3%，74.1% and 69.4%，respectively.However，there was no significant difference in AEG-1 expression among general clinical data including gender，eyes，age，optic disc involvement，secondary retinal detachment，epithelioid cell type and scleral duct invasion (all P>0.05).Conclusion　AEG-1 is overexpressed in UM and has a significant correlation with multiple clinical risk factors，suggesting that high expression of AEG-1 may be one of the important prognostic indicators for UM.

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备注/Memo

备注/Memo:
北京市眼科研究所重点学科引领计划资助项目（编号：201505）；北京市自然科学基金资助（编号：7162036）
更新日期/Last Update: 2018-02-02