[1]陈月芹,黄振平,程莹莹,等.姜黄素对核转录因子-κB表达的影响及小鼠角膜紫外线照射损伤的抑制作用[J].眼科新进展,2017,37(11):1015-1017.[doi:10.13389/j.cnki.rao.2017.0257]
　CHEN Yue-Qin,HUANG Zhen-Ping,CHENG Ying-Ying,et al.Effects of curcumin on NF-κB expression and UVA-induced corneal injury in mice[J].Recent Advances in Ophthalmology,2017,37(11):1015-1017.[doi:10.13389/j.cnki.rao.2017.0257]
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姜黄素对核转录因子-κB表达的影响及小鼠角膜紫外线照射损伤的抑制作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年11期
页码:
1015-1017
栏目:
实验研究
出版日期:
2017-11-05

文章信息/Info

Title:
Effects of curcumin on NF-κB expression and UVA-induced corneal injury in mice
作者:
陈月芹; 黄振平; 程莹莹; 薛春燕
210002　江苏省南京市，南京大学医学院临床学院 南京总医院眼科
Author(s):
CHEN Yue-Qin; HUANG Zhen-Ping; CHENG Ying-Ying; XUE Chun-Yan
School of Medicine，Nanjing University & Department of Ophthalmology，Nanjing General Hospital of Nanjing Military Command of PLA，Nanjing 210002，Jiangsu Province，China
关键词:
紫外线A; 角膜; 核转录因子-κB
Keywords:
ultraviolet A; cornea; nuclear factor-κB
分类号:
R772.2
DOI:
10.13389/j.cnki.rao.2017.0257
文献标志码:
A
摘要:
目的　探讨姜黄素对紫外线A（ultraviolet A，UVA）照射小鼠角膜损伤的抑制作用。方法　取C57 BL/6小鼠30只，随机分为3组，每组各10只；UVA照射组双眼接受位于前上方的UVA照射，波长为 320～400 nm，照射强度为 0.05 W·cm-2，照射距离固定，照射时间24 h。姜黄素干预组在UVA照射前3 d给予小鼠姜黄素30 mg·kg-1腹腔内注射，并持续至照射后48 h。对照组不做任何处理。利用裂隙灯观察角膜病变并对角膜混浊进行分级；照射后48 h取小鼠角膜组织，使用电泳迁移率实验检测核转录因子-κB （nuclear factor-κB，NF-κB）的表达。结果　照射后48 h，UVA照射组角膜混浊临床评分（3.10±0.74）均高于对照组（0）及姜黄素干预组（0.35±0.24）。两两比较发现对照组与姜黄素干预组间角膜混浊临床评分差异无统计学意义（P>0.05）；UVA照射组与对照组以及姜黄素干预组间差异均有统计学意义（均为P<0.05）。照射后48 h，角膜组织NF-κB表达水平对照组（1.25±0.13）与姜黄素干预组（1.58±0.34）均较低，UVA照射组（3.98±0.58）明显升高。两两比较发现对照组与姜黄素干预组角膜组织NF-κB表达水平差异无统计学意义（P>0.05）；UVA照射组与对照组以及姜黄素干预组间差异均有统计学意义（均为P<0.05）。结论　姜黄素可以减轻UVA照射对小鼠角膜造成的损伤，其作用可能是通过抑制角膜组织内NF-κB表达实现的。
Abstract:
Objective　To investigate the protective effect of curcumin (Cur) on mice cornea against ultraviolet A (UVA)-induced damage.Methods　Together 30 C57 BL/6 mice were randomly divided into 3 groups (10 mice in each group)：UVA irradiation group (UVA group)，UVA irradiation with curcumin treatment group (UVA + Cur group) and control group.Mice in the UVA group was exposed to UVA for 24 h，with the wavelength of 320-400 nm and the irradiation intensity of 0.05 W·cm-2.In UVA+Cur group，mice were given 30 mg·kg-1 curcumin intraperitoneally 3 days before irradiation and lasted until 48 h after irradiation.And the control group was left untreated.Then corneal opacity was evaluated and scored through slit-lamp biomicroscopy.After irradiation for 48 h，corneal tissue was collected for the detection of the expression of NF-κB by electrophoretic mobility shift assay.Results　After irradiation for 48 h，the score of corneal opacity in the UVA group (3.10±0.74) was higher than that of the control group (0) and UVA+Cur group (0.35±0.24) (P<0.05).The expression of NF-κB in cornea was detectable at a low level in the control group (1.25±0.13)，but was remarkably increased in UVA group (3.98±0.58) and suppressed by curcumin in UVA+Cur group (1.58±0.34)(P<0.05).There was no significant difference in corneal opacity score and NF-κB expression between control and UVA+Cur group (P>0.05)，but there was significant difference between control and UVA group and UVA+Cur and UVA group (all P<0.05).Conclusion　Curcumin can protect cornea against UVA-induced damage via inhibiting NF-κB activation.

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备注/Memo

备注/Memo:
南京总医院课题（编号：2016007）
更新日期/Last Update: 2017-11-01