[1]郑玲,冯光强.原发性先天性青光眼（PCG）患儿与正常婴幼儿血浆中微小核糖核酸（miRNA或miR）-29b、miR-24、miR-200c的表达差异及临床意义[J].眼科新进展,2017,37(8):755-758.[doi:10.13389/j.cnki.rao.2017.0191]
　ZHENG Ling,FENG Guang-Qiang.Expression differences and clinical significance of miR-29b,miR-24 and miR-200c in plasma of infants with PCG and normal[J].Recent Advances in Ophthalmology,2017,37(8):755-758.[doi:10.13389/j.cnki.rao.2017.0191]
点击复制

原发性先天性青光眼（PCG）患儿与正常婴幼儿血浆中微小核糖核酸（miRNA或miR）-29b、miR-24、miR-200c的表达差异及临床意义/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年8期
页码:
755-758
栏目:
应用研究
出版日期:
2017-08-05

文章信息/Info

Title:
Expression differences and clinical significance of miR-29b,miR-24 and miR-200c in plasma of infants with PCG and normal
作者:
郑玲; 冯光强
522000　广东省揭阳市，揭阳市人民医院眼科（郑玲）；510623　广东省广州市，广州市妇女儿童医疗中心眼科（冯光强）
Author(s):
ZHENG Ling; FENG Guang-Qiang
Department of Ophthalmology,Jieyang People’s Hospital(ZHENG Ling),Jieyang 522000,Guangdong Province,China;Department of Ophthalmology,Guangzhou Women and Children’s Medical Center(FENG Guang-Qiang),Guangzhou 510623,Guangdong Province,China
关键词:
微小核糖核酸-29 b; 微小核糖核酸-24; 微小核糖核酸-200c; 血浆; 原发性先天性青光眼
Keywords:
microRNA-29 b; microRNA-24; microRNA-200 c; plasma; primary congenital glaucoma
分类号:
R775.2
DOI:
10.13389/j.cnki.rao.2017.0191
文献标志码:
A
摘要:
目的　探讨原发性先天性青光眼（primary congenital glaucoma,PCG）患儿与正常婴幼儿血浆中微小核糖核酸（miRNA或miR）-29b、miR-24、miR-200c的表达差异及临床意义。方法　收集2015年至2017年散发的PCG患儿16例血浆标本作为PCG组，另选年龄相近的正常婴幼儿共49例血浆标本作为正常人组。应用实时荧光定量PCR检测两组受试者血浆中miR-29b、miR-24、miR-200c的表达量，分析各自表达差异与病变严重程度的关系,并运用ROC曲线评价其诊断价值。结果　PCG组受试者血浆中miR-29b、miR-24、miR-200c相对表达量(0.31±0.19、0.17±0.16、0.55±0.18）明显低于正常人组（1.18±0.52、2.86±2.65、1.62±0.76）,差异均有统计学意义(均为P＜0.05)；血浆中miR-24、miR-29b的表达与PCG病变严重程度有关，病变越严重，其表达量越低；ROC曲线表明miR-24和miR-29b对PCG疾病的诊断价值高于miR-200c。结论　血浆中游离的miRNA可作为辅助诊断PCG一种新的血浆标志物。
Abstract:
Objective　To identify the expression differences of mircoRNA-29 b(miR-29b),microRNA?24(miR-24) and microRNA-200 c(miR-200c) in plasma of infants with primary congenital glaucoma (PCG) and normal,and analyze its clinical significance.Methods　The expression quantity of microRNAs (miR-29b,miR-24,miR-200c) in plasma of PCG group (16 cases) and normal control group (49 cases) were detected by RT-PCR,and the relationship between their expression differences and severity of disease were analyzed.The diagnostic value of miRNAs for PCG was evaluated by receiver-operating characteristic curve (ROC).Results　The expression quantity of miR-29b,miR-24，miR-200c in PCG group (0.31±0.19,0.17±0.16,0.55±0.18,respectively) were significantly lower than those in normal control group (1.18±0.52,2.86±2.65,1.62±0.76,respectively) (all P＜0.05);The expression quantity of miR-24 and miR-29b in plasma was related to the severity of PCG,the more severe the disease,the lower the expression;ROC curve indicated that miR-24 and miR-29b had a higher diagnostic value for PCG disease than miR-200c.Conclusion　Free miRNAs in plasma may be used as a new plasma markers for auxiliary diagnosis of PCG.

参考文献/References:

［1］　MARIANA LQ,REINHARD R,JUTTA M,ARNDT B,THOMAS T.New microRNAs from mouse and human［J］.RNA，2003,9(2):175-179.
［2］　郑玲，冯光强.小梁细胞表达的miRNA研究新进展［J］.眼科新进展，2014,34（11）：1094-1096.
ZHENG L,FENG GQ.Research advances in miRNA in human trabecular meshwork cells［J］.Rec Adv Ophthalmol,2014,34(11):1094-1096.
［3］　LUNA C,LI G,QIU J,EPSTEIN DL,GONZALEZ P.Cross-talk between miR-29 and transforming growth factor-betas in trabecular meshwork cells［J］.Invest Ophthalmol Vis Sci,2011,52(6):3567-3572.
［4］　LUNA C,LI G,QIU J,EPSTEIN DL,GONZALEZ P.MicroRNA-24 regulates the processing of latent TGFβ1 during cyclic mechanical stress in human trabecular meshwork cells through direct targeting of FURIN［J］.J Cell Physiol,2011,226(5):1407-1414.
［5］　LUNA C,LI G,HUANG J,QIU J,WU J,YUAN F,et al..Regulation of trabecular meshwork cell contraction and intraocular pressure by miR-200c［J］.PLoS One,2012,7(12):e51688.
［6］　MITCHELL PS,PARKIN RK,KROH EM,FRITZ BR,WYMAN SK,POGOSOVA-AGADJANYAN EL,et al.Circulating micro-RNAs as stable blood-based markers for cancer detection［J］.Proc Natl Acad Sci USA,2008,105(30):10513-10518.
［7］　CHEN X,BA Y,MA L,CAI X,YIN Y,WANG K,et al.Characterization of microRNAs in serum:a novel class of biomarkers for diagnosis of cancer and other diseases［J］.Cell Res,2008,18(10):997-1006.
［8］　WEBER JA,BAXTER DH,ZHANG S,HUANG DY,HUANG KH,LEE MJ,et al.The microRNA spectrum in 12 body fluids［J］.Clin Chem,2010,56(11):1733-1741.
［9］　TANAKA Y,TSUDA S,KUNIKATA H,SATO J,KOKUBUN T,YASUDA M,et al.Profiles of extracellular miRNAs in the aqueous humor of glaucoma patients assessed with a microarray system［J］.Sci Rep,2014,4:5089.
［10］　ZUBAKOV D,BOERSMA AW,CHOI Y,VAN KUIJK PF,WIEMER EA,KAYSER M.MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation［J］.Int J Legal Med,2010,124(3):217-226.
［11］　CORTEZ MA,BUESO-RAMOS C,FERDIN J,LOPEZ-BERESTEIN G,SOOD AK,CALIN GA.MicroRNAs in body fluids--the mix of hormones and biomarkers［J］.Nat Rev Clin Oncol,2011,8(8):467-477.
［12］　DUNMIRE JJ,LAGOUROS E,BOUHENNI RA,JONES M,EDWARD DP.MicroRNA in aqueous humor from patients with cataract［J］.Exp Eye Res,2013,108(3):68-71.
［13］　LUNA C,LI G,QIU J,EPSTEIN DL,GONZALEZ P.Role of miR-29b on the regulation of the extracellular matrix in human trabecular meshwork cells under chronic oxidative stress［J］.Mol Vis,2009,15:2488-2497.
［14］　VILLARREAL G JR,OH DJ,KANG MH,RHEE DJ.Coordinated regulation of extracellular matrix synthesis by the microRNA-29 family in the trabecular meshwork［J］.Invest Ophthalmol Vis Sci,2011,52(6):3391-3397.
［15］　王琰玲,王子露,杨迷芳,程杰，吴煜农.微小RNA-29 b体外抑制皮肤成纤维细胞胶原蛋白1的研究［J］.中华实验外科杂志,2011,4(28):540-542.
WANG YL,WANG ZL,YANG MF,CHENG J,WU YN.Inhibitory effects of microRNA-29 b on collagen 1 in skin fibroblasts［J］.Chin J Exp Surg,2011,4(28):540-542.
［16］　ZANON-MORENO V,MARCO-VENTURA P,LLEO-PEREZ A,PONS-VAZQUEZ S,GARCIA-MEDINA JJ,VINUESA-SILVA I,et al.Oxidative stress in primary open-angle glaucoma［J］.J Glaucoma,2008,17(4):263-268.
［17］　CHHABRA R,DUBEY R,SAINI N.Cooperative and individualistic functions of the microRNAs in the miR-23 a~27 a~24-2 cluster and its implication in human diseases［J］.Mol Cancer,2010,9:232.
［18］　BLAKYTNY R,LUDLOW A,MARTIN GE,IRELAND G,LUND LR,FERGUSON MW,et al.Latent TGF-beta1 activation by platelets［J］.J Cell Physiol,2004,199(1):67-76.
［19］　DOGAR AM,TOWBIN H,HALL J.Suppression of latent transforming growth factor(TGF)-beta1 restores growth inhibitory TGF-beta signaling through microRNAs［J］.J Biol Chem,2011,286(18):16447-16458.
［20］　TSUCHIYA Y,NAKAJIMA M,TAKAGI S,TANIYA T,YOKOI T.MicroRNA regulates the expression of human cytochrome P4501 B1［J］.Cancer Res,2006,66(18):9090-9098.
［21］　OGUETA SB,SCHWARTZ SD,YAMASHITA CK,FARBER DB.Estrogen receptor in the human eye:influence of gender and age on gene expression［J］.Invest Ophthalmol Vis Sci,1999,40(9):1906-1911.
［22］　TANG YM,CHEN GF,THOMPSON PA,LIN DX,LANG NP,KADLUBAR FF.Development of an antipeptide antibody that binds to the C-terminal region of human CYP1 B1［J］.Drug Metab Dispos,1999,27(2):274-280.
［23］　JANSSON I,STOILOV I,SARFARAZI M,SCHENKMAN JB.Effect of two mutations of human CYP1 B1,G61 E and R469 W,on stability and endogenous steroid substrate metabolism［J］.Pharmacogenetics,2001,11(9):793-801.
［24］　MEISLER NT,PARRELLI J,GENDIMENICO GJ,MEZICK JA,CUTRONEO KR.All-trans-retinoic acid inhibition of Pro alpha1(I) collagen gene expression in fetal rat skin fibroblasts:identification of a retinoic acid response element in the Pro alpha1(I) collagen gene［J］.J Invest Dermatol,1997,108(4):476-481.
［25］　RAGUSA M,CALTABIANO R,RUSSO A,PUZZO L,AVITABILE T,LONGO A,et al.MicroRNAs in vitreus humor from patients with ocular diseases［J］.Mol Vis,2013,19:430-440.
［26］　TANIHARA H,INATANI M,HONJO M,TOKUSHIGE H,AZUMA J,ARAIE M.Intraocular pressure-lowering effects and safety of topical administration of a selective ROCK inhibitor,SNJ-1656,in healthy volunteers［J］.Arch Ophthalmol,2008,126(3):309-315.

备注/Memo

备注/Memo:
广东省卫生厅科技项目（编号：A2014567）
更新日期/Last Update: 2017-08-14