[1]姜凌峰,石栋,阎启昌.2型糖尿病合并皮质性白内障患者房水中Txnip、Trx的定量检测及相关性分析[J].眼科新进展,2017,37(8):736-738.[doi:10.13389/j.cnki.rao.2017.0186]
　JIANG Ling-Feng,SHI Dong,YAN Qi-Chang.Quantitative detection and correlation analysis of Txnip，Trx in aqueous humor of cortical cataract patients with type 2 diabetes[J].Recent Advances in Ophthalmology,2017,37(8):736-738.[doi:10.13389/j.cnki.rao.2017.0186]
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2型糖尿病合并皮质性白内障患者房水中Txnip、Trx的定量检测及相关性分析/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年8期
页码:
736-738
栏目:
应用研究
出版日期:
2017-08-05

文章信息/Info

Title:
Quantitative detection and correlation analysis of Txnip，Trx in aqueous humor of cortical cataract patients with type 2 diabetes
作者:
姜凌峰; 石栋; 阎启昌
110005　辽宁省沈阳市，中国医科大学附属第四医院眼科，中国医科大学附属眼科医院，辽宁省晶状体学重点实验室
Author(s):
JIANG Ling-Feng; SHI Dong; YAN Qi-Chang
Department of Ophthalmology，the Forth Affiliated Hospital，China Medical University，the Eye Hospital of China Medical University，the Key Laboratory of Lens in Liaoning Province，Shenyang 110005，Liaoning Province，China
关键词:
糖尿病性白内障; 硫氧还蛋白; 硫氧还蛋白互作蛋白; 氧化应激
Keywords:
diabetic cataract; Thioredoxin; Thioredoxin interacting protein; oxidative stress
分类号:
R776.1
DOI:
10.13389/j.cnki.rao.2017.0186
文献标志码:
A
摘要:
目的　探讨2型糖尿病合并皮质性白内障的患者房水中硫氧还蛋白（thioredoxin，Trx）、硫氧还蛋白互作蛋白（thioredoxin interacting protein，Txnip）的含量以及与皮质性白内障程度的相关性。方法　采用酶联免疫吸附实验（ELISA法）测定60例（60眼）明确诊断2型糖尿病合并皮质性白内障患者的房水中Trx及Txnip的含量，其中按照患者糖化血红蛋白（hemoglobin A1c,HbA1c）值将患者分组：A组(对照组,20例)HbA1c＜5.5%；B组(20例)5.5%≤HbA1c＜6.5%和C组(20例)HbA1c≥6.5%。结果　与A组相比，B组患者房水中Txnip浓度增高，而C组浓度最高，差异均有统计学意义（均为P＜0.05）；与A组相比，B组Trx的浓度升高，而C组Trx的浓度最低，差异均有统计学意义（均为P＜0.05）。A组中Trx与Txnip的浓度呈正相关（r=0.810，P=0.000）；在C组中，Trx与Txnip的浓度呈负相关（r=-0.809，P=0.000），与白内障程度呈负相关（r=-0.727，P=0.001），其余均不相关（均为P＞0.05）。Logistic回归分析显示，Txnip和HbA1c是白内障发病的危险因素。结论　Trx、Txnip可能参与了糖尿病患者中白内障的发生发展。
Abstract:
Objective　To investigate the level and correlation between Thioredoxin (Trx) and Thioredoxin interacting protein (Txnip) in aqueous humor of cortical cataract patients with type 2 diabetes.Methods　The Txnip and Trx levels were determined by Elisa in aqueous humor of 60 cataract patients with type 2 diabetes,which were divided into group A (control group,HbA1c＜5.5%)，group B (5.5%≤HbA1c＜6.5%) and group C (HbA1c≥6.5%),and each group contains 20 patients.Results　The level of Txnip in group B was higher than that in group A,while that in group C was the highest,there were statistical differences (all P＜0.05).Compared with group A,the level of Trx in group B was increased,while that in group C was the lowest,there were statistical differences (all P＜0.05).The level of Trx was positive correlated with Txnip in group A (r=0.810,P=0.000）,but negative correlated with Txnip in group C (r=-0.809,P=0.000) and degree of cataract in group C（r=-0.727,P=0.001）.Logistic regression analysis revealed that Txnip and HbA1c were risk factors of cortical cataract.Conclusion　Trx and Txnip may play an important role in the development of diabetic cataract.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（编号：81600777）
更新日期/Last Update: 2017-08-14