[1]马明洋,袁晴,刘启,等.羊膜泪道支架预防去势雄兔干眼症的实验研究[J].眼科新进展,2017,37(8):709-713.[doi:10.13389/j.cnki.rao.2017.0180]
　MA Ming-Yang,YUAN Qing,LIU Qi,et al.Experimental study of amniotic lacrimal duct stent used to prevent dry eye of castrated rabbits[J].Recent Advances in Ophthalmology,2017,37(8):709-713.[doi:10.13389/j.cnki.rao.2017.0180]
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羊膜泪道支架预防去势雄兔干眼症的实验研究/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年8期
页码:
709-713
栏目:
实验研究
出版日期:
2017-08-05

文章信息/Info

Title:
Experimental study of amniotic lacrimal duct stent used to prevent dry eye of castrated rabbits
作者:
马明洋; 袁晴; 刘启; 刘康成; 朱佩文; 康红花; 姜楠; 叶蕾; 裴重刚; 邵毅
330006　江西省南昌市，南昌大学第一附属医院眼科
Author(s):
MA Ming-Yang; YUAN Qing; LIU Qi; LIU Kang-Cheng; ZHU Pei-Wen; KANG Hong-Hua; JIANG Nan; YE Lei; PEI Chong-Gang; SHAO Yi
Department of Ophthalmology，the First Affiliated Hospital of Nanchang University，Nanchang 330006，Jiangxi Province，China
关键词:
羊膜泪道支架; 干眼症; 去势雄兔
Keywords:
amniotic lacrimal duct stent; xerophthalmia; castrated rabbits
分类号:
R777.3
DOI:
10.13389/j.cnki.rao.2017.0180
文献标志码:
A
摘要:
目的　探究羊膜泪道支架对雄兔干眼症的预防效果。方法　选取36只健康雄兔，剪除第三眼睑并抗感染处理，随机分为3组（每组12只），制作去势雄兔模型，其中A组为阴性对照组，B组为干眼模型组，C 组为泪道羊膜预防组，并在植入羊膜泪道支架前、植入后2周、4周及6周行角膜荧光素(fluorescent，FL)染色、Schirmer I试验、Western blot、免疫荧光染色及角膜共聚焦显微镜检查。结果　三组不同时间点间的泪液分泌量及FL有明显改变（F=7.126，P=0.009；F=9.658，P=0.016），三组间泪液分泌量及FL有明显改变（F=12.582，P=0.005；F=13.187，P=0.013），三组的泪液分泌量及FL变化趋势也有明显改变（F=8.531，P=0.007；F=10.652，P=0.019）。A、B、C三组植入后6周上皮基底细胞分别（3811±414）个细胞、（3820±314）个细胞、（2789±353）个细胞，炎症细胞密度分别为（266±28）个细胞、（266±29）个细胞、（67±13）个细胞；A、B、C三组植入后6周上皮基底细胞（F=13.442，P=0.012）和炎症细胞密度（F=9.231，P=0.021）比较，差异均有统计学意义。泪道上皮K16染色均为阴性，植入羊膜泪道支架后各时间点 A、B、C 三组兔泪小管组织中 α-SMA 表达无明显改变，差异有统计学意义（F=14.681，P= 0.002）。结论　植入羊膜泪道支架对雄兔干眼症的预防有一定的效果。
Abstract:
Objective　To explore the effects of amniotic lacrimal duct stenting on the prevention of dry eye in castrated rabbits.Methods　Thirty-six healthy male rabbits were selected,the third eyelid were cut off and anti-infection treatment were given,which were randomly divided into 3 groups (12 cases in each group),the castrated male rabbits models were made.Among them,group A was negative control group,group B was dry eye model group,group C was group of lacrimal amniotic membrane group.At 2 weeks before implantation of amniotic lacrimal duct stent,2 weeks,4 weeks and 6 weeks after implantation,the fluorescent（FL）examination,Western blot,Schirmer I examination,immunofluorescence staining and corneal confocal microscopy were performed.Results　The levels of tear secretion and FL in the three groups among different time points were significantly different (F=7.126,P= 0.009;F=9.658,P=0.016),and there were significant differences among three groups (F=12.582,P=0.005;F=13.187,P=0.013).The tendency of tear secretion and FL in the three groups were also significantly changed (F=8.531,P=0.007;F=10.652,P=0.019).The epithelial basal cells at 6 weeks after implantation in three groups were 3811±414,3820±314,2789±353,and the density of inflammatory cells was 266 ± 28,266 ± 29,67±13,there were significant differences among three groups (F=13.442,P=0.012;F=9.231,P=0.021).The K16 staining in the duct epithelium were negative,and the expression of α-SMA in the lacrimal duct tissue of group A,B and C was not changed at all time points after implantation of amniotic lacrimal stent,and there was no significant difference (F=14.681,P=0.002).Conclusion　The amniotic lacrimal stent implantation has certain effect on the prevention of dry eye in rabbit.

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国家自然科学基金资助（编号：81660158、81160118、81160148、81460092、81400372、81400424）；江西省远航工程（编号：2014022）；江西省自然科学基金重大项目（编号：2016ACB21017）；江西省青年科学基金（编号：20151BAB215016、20161BAB215198）；江西省重点研发项目（编号：20151BBG70223）；江西省教育厅重点项目（编号：GJJ160020）；江西省教育厅科技计划项目（编号：GJJ160122）；江西省卫计委科技计划项目（编号：20164017、20175115、20175116）；江西省卫计委中医药科技计划项目（编号：20150823）
更新日期/Last Update: 2017-08-14