[1]祁海燕,苏学刚.LncRNA MT1JP对葡萄膜黑色素瘤细胞迁移和侵袭的影响[J].眼科新进展,2017,37(6):531-534.[doi:10.13389/j.cnki.rao.2017.0134]
　QI Hai-Yan,SU Xue-Gang.Effects of LncRNA MT1JP on migration and invasion of uveal melanoma cells[J].Recent Advances in Ophthalmology,2017,37(6):531-534.[doi:10.13389/j.cnki.rao.2017.0134]
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LncRNA MT1JP对葡萄膜黑色素瘤细胞迁移和侵袭的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年6期
页码:
531-534
栏目:
实验研究
出版日期:
2017-06-05

文章信息/Info

Title:
Effects of LncRNA MT1JP on migration and invasion of uveal melanoma cells
作者:
祁海燕; 苏学刚
101300　北京市，北京市顺义区妇幼保健院眼科
Author(s):
QI Hai-Yan; SU Xue-Gang
Department of Ophthalmology，the Maternal and Child Care Service Center of Shunyi District，Beijing 101300，China
关键词:
LncRNA MT1JP; 葡萄膜黑色素瘤; 细胞迁移; 细胞侵袭
Keywords:
LncRNA MT1JP; uveal melanoma; cell migration; cell invasion
分类号:
R773.9
DOI:
10.13389/j.cnki.rao.2017.0134
文献标志码:
A
摘要:
目的　研究LncRNA MT1JP对葡萄膜黑色素瘤细胞迁移和侵袭的影响及机制。方法　实时荧光定量PCR测定LncRNA MT1JP在葡萄膜黑色素瘤细胞系SP6.5、M23及正常视网膜色素上皮细胞系ARPE-19中的表达，将SP6.5细胞分成3组，即MT1JP组、siMT1JP组和NC组，分别转染pcDNA3.1-MT1JP、pcDNA3.1-siMT1JP及pcDNA3.1空载体，采用细胞划痕实验和Transwell实验测定3组划痕愈合率和侵袭细胞数，Western blot测定P53蛋白表达水平。结果　葡萄膜黑色素瘤细胞系SP6.5和M23中LncRNA MT1JP的相对表达量分别为0.20±0.02和0.31±0.01，均显著低于正常视网膜色素上皮细胞系ARPE-19的1.0（均为P<0.01）。siMT1JP组划痕愈合率为61.5%±3.7%，MT1JP组为10.6%±1.7%，NC组为20.0%±2.1%，3组间两两相比差异均有统计学意义（均为P<0.01）。在200倍视野下，siMT1JP组侵袭细胞数为（75.6±6.8）个，MT1JP组侵袭细胞数为（10.3±2.6）个，NC组为（29.50±3.9）个，3组间两两相比差异均有统计学意义（均为P<0.01）。siMT1JP组P53蛋白相对表达量为0.41±0.04，显著低于NC组的1.0（P<0.01）；MT1JP组P53蛋白相对表达量为5.73±0.62，显著高于NC组的1.0（P<0.01）。结论　LncRNA MT1JP抑制葡萄膜黑色素瘤细胞的转移和侵袭，其机制可能与上调P53蛋白表达有关。
Abstract:
Objective　To investigate the effects of LncRNA MT1JP on the migration and invasion of uveal melanoma cell，and explore its regulatory mechanism.Methods　The expression level of LncRNA MT1JP in uveal melanoma cell line and normal retinal pigment epithelium cell line were measured by qRT-PCR.The SP6.5 cell line was divided into three groups，MT1JP group transfect with pcDNA3.1-MT1JP，siMT1JP group transfect with pcDNA3.1-siMT1JP and NC group transfect with negative pcDNA3.1 plasmid.The migration and invasion ability were measured by cell wound scratch assay and transwell assay.Western blot was used to measure the expression level of P53 protein.Results　The expression level of LncRNA MT1JP in SP6.5 and M23 cell line was 0.20±0.02 and 0.31±0.01，respectively，which was significantly lower than 1.0 in ARPE-19 cell line (all P<0.01).The wound healing rate in siMT1JP group was 61.50±3.70%.while，NC group was 20.0%±2.10%，and MT1JP group was 10.6%±1.7%，there was no statistical difference among three groups (all P<0.01).The invasion cell number was 75.6±6.8 in siMT1JP group，which was significantly more than 29.5±3.9 in NC group，and 10.3±2.6 in MT1JP group，there was no statistical difference among three groups (all P<0.01).The expression level of P53 protein in siMT1JP group was 0.41±0.04，which was significantly lower than 1.0 in NC group (P<0.01).However，the expression level of P53 protein in MT1JP group was 5.73±0.62，which was significantly higher than 1.0 in NC group (P<0.01).Conclusion　LncRNA MT1JP inhibits the migration and invasion of uveal melanoma cell，which may be associated with up-regulation of P53 expression.

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更新日期/Last Update: 2017-06-28