[1]陈静,陈艺,邹秀兰,等.青蒿琥酯对人Tenon囊成纤维细胞增殖与凋亡的影响[J].眼科新进展,2017,37(6):523-526.[doi:10.13389/j.cnki.rao.2017.0132]
　CHEN Jing,CHEN Yi,ZOU Xiu-Lan,et al.Effects of artesunate on cell proliferation and apoptosis of human Tenon’s capsule fibroblasts[J].Recent Advances in Ophthalmology,2017,37(6):523-526.[doi:10.13389/j.cnki.rao.2017.0132]
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青蒿琥酯对人Tenon囊成纤维细胞增殖与凋亡的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年6期
页码:
523-526
栏目:
实验研究
出版日期:
2017-06-05

文章信息/Info

Title:
Effects of artesunate on cell proliferation and apoptosis of human Tenon’s capsule fibroblasts
作者:
陈静; 陈艺; 邹秀兰; 邹玉平
510010　广东省广州市，广州军区广州总医院眼科（陈静，邹秀兰，邹玉平）；510010　广东省广州市，广州军区广州总医院培训中心（陈艺）
Author(s):
CHEN Jing; CHEN Yi; ZOU Xiu-Lan; ZOU Yu-Ping
Department of Ophthalmology，Guangzhou General Hospital of Guangzhou Command(CHEN Jing，ZOU Xiu-Lan，ZOU Yu-Ping)，Guangzhou 510010，Guangdong Province，China；Department of Training Center，Guangzhou General Hospital of Guangzhou Command(CHEN Yi)，Guangzhou 510010，Guangdong Province，China
关键词:
青蒿琥酯; 人Tenon囊成纤维细胞; 细胞增殖; 细胞凋亡; 青光眼
Keywords:
artesunate; human Tenon’s capsule fibroblasts; cell proliferation; cell apoptosis; glaucoma
分类号:
R775.9
DOI:
10.13389/j.cnki.rao.2017.0132
文献标志码:
A
摘要:
目的　观察青蒿琥酯(artesunate，Art) 对人Tenon 囊成纤维（human Tenon’s capsule fibroblasts，HTFs）细胞增殖与凋亡的影响，探讨青光眼术后滤过泡瘢痕化的治疗对策。方法　体外培养HTFs细胞，应用不同浓度(50 μg·mL-1、100 μg·mL-1、150 μg·mL-1、200 μg·mL-1)的Art处理细胞，采用MTT法检测Art对细胞增殖的影响，流式细胞仪检测Art对细胞凋亡的影响，Western blot检测Art对凋亡相关蛋白Bax、Bcl-2表达的影响。结果　Art作用细胞48 h后，MTT结果显示：与空白对照组比较，不同浓度的Art处理组对HTFs细胞的增殖均有抑制作用，且呈浓度依赖性抑制(均为P<0.05)。流式细胞仪结果显示：与空白对照组比较，50 μg·mL-1、100 μg·mL-1、150 μg·mL-1、200 μg·mL-1 Art作用后细胞凋亡率逐渐增加(均为P<0.05)，分别为8.80%±0.88%、11.60%±0.56%、16.30%±1.03%、23.40%±1.62%。Western blot检测结果显示：与空白对照组比较，不同浓度Art处理组Bax蛋白表达明显增加，Bcl-2蛋白表达明显降低，且均呈浓度依赖性(均为P<0.05)。结论　Art可抑制HTFs细胞增殖并诱导其凋亡，其机制可能是通过调节凋亡相关蛋白Bax、Bcl-2的表达而实现的。Art可能成为一种潜在的抗青光眼术后滤过泡瘢痕化的药物。
Abstract:
Objective　To investigate the effects of artesunate (Art) on cell proliferation and apoptosis of human Tenon’s capsule fibroblasts (HTFs)，and discuss the countermeasures of bleb scarring in glaucoma.Methods　In vitro，HTFs were cultivated and applicated by different concentrations (50 μg·mL-1，100 μg·mL-1，150 μg·mL-1，200 μg·mL-1) of Art for 48 hours.The effect of Art on cell proliferation was assessed by MTT method．The rate of apoptosis induced by Art was determined by flow cytometry.Western Blot was performed to detect the relative expression levels of Bax and Bcl-2 after Art was treated．Results　After treated with Art for 48 hours，compared with blank control group，Art (50 μg·mL-1，100 μg·mL-1，150 μg·mL-1，200 μg·mL-1) group exhibited notable anti-proliferative effect on HTFs with concentration-dependence (all P<0.05).The results of flow cytometry showed that the apoptosis rates (8.80%±0.88%，11.60%±0.56%，16.30%±1.03%，23.40%±1.62%) of HTFs were significantly enhanced with the increase of Art concentration (all P<0.05).The relative expression levels of Bax were obviously high with the increase of Art concentration，while Bcl-2 levels were significantly low with the increase of Art concentration (all P<0.05).Conclusion　Art can inhibit the proliferation and induce cell apoptosis of HTFs possibly by enhancing the expression of Bax and reducing the expression of Bcl-2.Art may be a potential drug in preventing fibrous scar formation after glaucoma filtration surgery.

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更新日期/Last Update: 2017-06-28