[1]罗鑫,刘太祥,任荟璇,等.硫化氢对H2O2诱导的视网膜神经节细胞氧化损伤的保护作用[J].眼科新进展,2017,37(6):515-518.[doi:10.13389/j.cnki.rao.2017.0130]
 LUO Xin,LIU Tai-Xiang,REN Hui-Xuan,et al.Protective effects of H2S against H2O2-induced oxidative injury in RGC[J].Recent Advances in Ophthalmology,2017,37(6):515-518.[doi:10.13389/j.cnki.rao.2017.0130]
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硫化氢对H2O2诱导的视网膜神经节细胞氧化损伤的保护作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年6期
页码:
515-518
栏目:
实验研究
出版日期:
2017-06-05

文章信息/Info

Title:
Protective effects of H2S against H2O2-induced oxidative injury in RGC
作者:
罗鑫刘太祥任荟璇罗艳
563003 贵州省遵义市,遵义医学院附属医院贵州省眼科医院
Author(s):
LUO XinLIU Tai-XiangREN Hui-XuanLUO Yan
Guizhou Eye Hospital,Affiliated Hospital of Zunyi Medical University,Zunyi 563003,Guizhou Province,China
关键词:
氧化应激视网膜神经节细胞硫化氢视神经萎缩蛋白1
Keywords:
oxidative stressretinal ganglion cellhydrogen sulfideoptic atrophy 1
分类号:
R774
DOI:
10.13389/j.cnki.rao.2017.0130
文献标志码:
A
摘要:
目的 探讨硫化氢对H2O2诱导的视网膜神经节细胞(retinal ganglion cell,RGC)-5氧化损伤的保护作用及可能机制。方法 将RGC-5分为4组,RGC-5组为正常对照组;RGC-5+H2O2组:RGC-5在500 μmol·L-1 H2O2中培养24 h诱导氧化损伤;RGC-5+NaHS+H2O2组:RGC-5置于50 μmol·L-1 NaHS中30 min后在500 μmol·L-1 H2O2中培养24 h;RGC-5+NaHS组:RGC-5置于50 μmol·L-1 NaHS中30 min。Western blot检测线粒体内、外细胞色素C和视神经萎缩蛋白1(optic atrophy 1,OPA1)表达;用荧光探针JC-1检测线粒体膜电位,用透射电镜观察线粒体形态。结果 与RGC-5组相比,RGC-5+H2O2组RGC-5细胞质内细胞色素C表达增加,而线粒体内的OPA1表达减少(均为P<0.05)。RGC-5组和RGC-5+NaHS+H2O2组线粒体内、外细胞色素C的表达差异均无统计学意义(均为P>0.05)。与RGC-5组相比,RGC-5+NaHS组细胞质内细胞色素C表达减少,而线粒体内的细胞色素C表达增加(均为P<0.05)。与RGC-5组相比,RGC-5+H2O2组RGC-5细胞质内OPA1表达显著增加,而线粒体内的OPA1表达减少(均为P<0.05)。RGC-5+NaHS+H2O2组和RGC-5+NaHS组RGC-5线粒体内、外OPA1的表达与RGC-5组相似,差异均无统计学意义(均为P>0.05)。RGC-5+H2O2组线粒体膜电位与其他3组比较明显下降,其余3组间线粒体膜电位比较,差异无统计学意义(P>0.05)。RGC-5+H2O2组线粒体肿胀呈球状,而其余3组线粒体肿胀不明显。结论 硫化氢可能通过阻止线粒体释放OPA1来减轻H2O2导致的RGC-5氧化损伤。
Abstract:
Objective To investigate the protective effects and possible mechanism of hydrogen sulfide (H2S) against oxidative stress injury induced by hydrogen peroxide (H2O2) in retinal ganglion cell-5 (RGC-5).Methods RGC-5 cells were divided into four groups:RGC-5 group (normal control group),RGC-5+H2O2 (RGC-5 were cultured in 500 μmol·L-1 H2O2 for 24 hours) group,RGC-5+NaHS (RGC-5 were cultured in 50 μmol·L-1 NaHS for 30 minutes)+ H2O2 (RGC-5 were cultured in 500 μmol·L-1 H2O2 for 24 hours) group,and RGC-5+NaHS (RGC-5 were cultured in 50 μmol·L-1 NaHS for 30 minutes) group.Western blots were applied to measure the expression of cytochrome c (Cyt.c) and optic atrophy 1 (OPA1).The fluorescent dye JC-1 assay was chosen to detect the mitochondrial membrane potential (ΔΨm).Furthermore,transmission electron microscope was used to observe the morphology of mitochondria.Results Compared with RGC-5 group,the expression of Cyt.c in RGC-5+H2O2 group decreased in mitochondria,and increased in cytoplasm (all P<0.05),but there was no statistical difference between RGC-5 group and RGC-5+NaHS+ H2O2 group (all P>0.05).Compared with RGC-5 group,the expression of Cyt.c in RGC-5+NaHS group increased in mitochondria,and decreased in cytoplasm (all P<0.05).Compared with RGC-5 group,the expression of OPA1 in RGC-5+H2O2 group decreased in mitochondria,and increased in cytoplasm (all P<0.05).In RGC-5+ NaHS+ H2O2 group and RGC-5+NaHS group,the expression of OPA1 within and outside the mitochondria had no significant difference compared with RGC-5 group (all P>0.05).Compared with other three groups,the mitochondrial membrane potential in RGC-5+H2O2 group obviously decreased,but there was no statistical difference among other three groups (P>0.05).The mitochondria were globular swelled in RGC-5 group,but in other three groups,the mitochondria had slightly swelled.Conclusion H2S can protect the mitochondrial morphology and functions of RGC?5 from H2O2-induced oxidative stress via preventing OPA1 release from mitochondria.

参考文献/References:

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备注/Memo

备注/Memo:
贵州省科学技术基金资助(编号:20147588);遵义医学院博士启动基金(编号:201307)
更新日期/Last Update: 2017-06-28