[1]王松涛,肖虹蕾,周国民.一种稳定标记小鼠视网膜内两种不同类型神经节细胞的方法[J].眼科新进展,2017,37(2):114-116.[doi:10.13389/j.cnki.rao.2017.0030]
　WANG Song-Tao,XIAO Hong-Lei,ZHOU Guo-Min.A stable method to label two different types of retinal ganglion cells in mouse retina[J].Recent Advances in Ophthalmology,2017,37(2):114-116.[doi:10.13389/j.cnki.rao.2017.0030]
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一种稳定标记小鼠视网膜内两种不同类型神经节细胞的方法/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年2期
页码:
114-116
栏目:
实验研究
出版日期:
2017-02-05

文章信息/Info

Title:
A stable method to label two different types of retinal ganglion cells in mouse retina
作者:
王松涛; 肖虹蕾; 周国民
453003　河南省新乡市，新乡医学院人体解剖学教研室（王松涛）；200032　上海市，复旦大学上海医学院人体解剖与组织胚胎学系（王松涛，肖虹蕾，周国民）
Author(s):
WANG Song-Tao; XIAO Hong-Lei; ZHOU Guo-Min
Department of Human Anatomy,Xinxiang Medical University(WANG Song-Tao),Xinxiang 453003,Henan Province,China;Department of Human Anatomy,Histology and Embryology,Shanghai Medical College,Fudan University(WANG Song-Tao,XIAO Hong-Lei,ZHOU Guo-Min),Shanghai 200032,ChinaResponsible author:ZHOU Guo-Min,E-mail:gmzhou185@163.com;ORCID:0000-0001-5071-4528
关键词:
小鼠; 视网膜; 视网膜神经节细胞; 内在光敏视网膜神经节细胞
Keywords:
mouse; retina; retinal ganglion cell; intrinsically photosensitive retinal ganglion cells
分类号:
R774
DOI:
10.13389/j.cnki.rao.2017.0030
文献标志码:
A
摘要:
目的　报道一种简单高效显示小鼠视网膜中两种不同类型神经节细胞的方法。方法　利用特殊标记物Brn3a和Melanopsin，通过视网膜铺片免疫荧光双标染色结合激光共聚焦显微镜，分别标记小鼠视网膜中普通视网膜神经节细胞和内在光敏视网膜神经节细胞。结果　免疫荧光染色结果表明，内在光敏视网膜神经节细胞与普通视网膜神经节细胞均位于视网膜节细胞层，相间互补分布。内在光敏视网膜神经节细胞数量较少，为普通视网膜神经节细胞的1%~2%，其轴突朝向视神经盘方向汇集，树突野较大，伸向内网层。结论　免疫荧光双标染色是小鼠视网膜内两种不同类型视网膜神经节细胞简单易行、稳定高效的标记方法。
Abstract:
Objective　To report a simple and efficient method to label two different types of retinal ganglion cells (RGCs) in mouse retina.Methods　Eyeballs were harvested from normal adult C57 BL/6 J mouse,the retinas were isolated,four radial cuts were done,the retinas were pasted on the nitrocellulose membrane with the ganglion cell layer upturned.The immunofluorescence double staining and laser confocal microscope was used to reveal conventional retinal ganglion cells and intrinsically photosensitive retinal ganglion cells (ipRGCs) using Brn3a and Melanopsin.Results　The double staining results of whole mount retina showed that conventional RGCs and melanopsin immunopositive ipRGCs had a complementary distribution in mouse retina,these two subtypes of RGCs were predominantly present in the ganglion cell layer.The numbers of ipRGCs was just about 1%-2% of conventional RGCs,and the axons of ipRGCs toward the direction of the optic disc,several dendrites toward the inner plaximem layer.Conclusion　The immunofluorescence double staining of whole mount retina is a simple,stable and efficient method to label two different types of mouse RGCs.

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备注/Memo

备注/Memo:
 国家自然科学基金资助（编号：81170885）；新乡医学院博士科研基金资助（编号：XYBSKYZZ2015010-505119）
更新日期/Last Update: 2017-03-01