[1]张兰兰,刘琼,于健,等.转化生长因子-β(TGF-β)影响豚鼠巩膜成纤维细胞增殖和α-平滑肌肌动蛋白(α-SMA)表达的研究[J].眼科新进展,2016,36(11):1011-1015.[doi:10.13389/j.cnki.rao.2016.0270]
 ZHANG Lan-Lan,LIU Qiong,YU Jian,et al.Effects of TGF-[3 on cellular proliferation and a-SMA expression in guinea pig scleral fibroblasts[J].Recent Advances in Ophthalmology,2016,36(11):1011-1015.[doi:10.13389/j.cnki.rao.2016.0270]
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转化生长因子-β(TGF-β)影响豚鼠巩膜成纤维细胞增殖和α-平滑肌肌动蛋白(α-SMA)表达的研究
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年11期
页码:
1011-1015
栏目:
实验研究
出版日期:
2016-11-05

文章信息/Info

Title:
Effects of TGF-[3 on cellular proliferation and a-SMA expression in guinea pig scleral fibroblasts
作者:
张兰兰刘琼于健唐晓娟徐静
510515 广东省广州市,南方医科大学南方医院临床实验中心(张兰兰);510515 广东省广州市,南方医科大学南方医院眼科(刘琼,于健,唐晓娟,徐静)
Author(s):
ZHANG Lan-Lan LIU Qiong YU Jian TANG Xiao-Juan XU Jing
Clinic Experimental Center of Nanfang Hospital, Southem Medical University ( ZHANG Lan-Lan ) , Guangzhou 510515 , Guangdong Province , China ; Department of Ophthalmology , Nanfang Hospital, Southern Medical University ( LIU Qiong , YU Jian , TANG Xiao-Juan , XU Jing ) , Guangzhou 510515 , Guangdong Province , China
关键词:
转化生长因子-βα-平滑肌肌动蛋白细胞增殖近视
Keywords:
transforming growth factor- [3 alpha-smooth muscle actin proliferation myopia
分类号:
R772.3
DOI:
10.13389/j.cnki.rao.2016.0270
文献标志码:
A
摘要:
目的 本研究探讨转化生长因子-β(transforminggrowthfactor,TGF-β)体外刺激豚鼠巩膜成纤维细胞,观察豚鼠巩膜成纤维细胞增殖和α-平滑肌肌动蛋白(α-smoothmus-cleactin,α-SMA)的表达。方法 原代培养豚鼠巩膜成纤维细胞并鉴定,MTT法检测豚鼠巩膜成纤维细胞的增殖,培养液分别加入20ng·mL-1 TGF-β1、TGF-β2、TGF-β3,分别于12h、24h、48h、72h,Real-timePCR检测α-SMAmRNA,Western-blotting和免疫荧光化学法检测其蛋白表达。结果 与对照组相比,TGF-β1、TGF-β2和TGF-β3促进豚鼠巩膜成纤维细胞增殖,呈剂量依赖性(P<0.05),其中TGF-β1促进豚鼠巩膜成纤维细胞增殖作用最强(P<0.05);TGF-β1、TGF-β2和TGF-β3均增加α-SMA的表达(均为P<0.001),TGF-β3促进α-SMA表达作用最强(P<0.001)。结论 TGF-β通过调控细胞外基质合成参与调控巩膜组织重塑。
Abstract:
Objective To investigate the role of transforming growth factor-[3 (TGF-[3) in cellular proliferation and d¡ªsmooth muscle actin (d¡ªSMA) expression in guinea pig scleral fibroblasts ( GSF) . Methods Primary culture and identification of GSF were followed by the MMT cell proliferation assay to assess proliferation. The culture was supplemented with 20 ng . mL -l of TGF-[31 ,TGF-[32 and TGF-[33 , which were removed at 12 hours.24 hours ,48 hours and 72 hours. Real-time polymerase chain reaction was performed to evaluate the mRNA expression of dÒ»SMA. Western blotting and immunofluorescent staining were also conducted to examine expression of d¡ªSMA. Results TGF-[3 up-regulated GSF proliferation, and this proliferation was dose-dependent ( P < 0. 05 ) . while TGF- [31 demonstrated the strongest regulative effect ( P < 0. 05 ) . All three TGF-[3 isoforms enhanced the expression of d¡ªSMA ( all P < 0. 001) , wihile TGF-[33 demonstrated the strongest regulative effect (P < 0. 001 ) . Conclrision TGF-[3 participates in scleral remodeling through the regulation of extracellular matrix synthesis.

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备注/Memo

备注/Memo:
国家自然科学基金资助(编号:30901650);南方医院院长基金重点项目(编号:2012A002)
更新日期/Last Update: 2016-11-29