[1]韩治华,杨淑焕,郭卫民,等.TGF-β介导的Smad信号通路在增生型糖尿病视网膜病变中的作用和意义[J].眼科新进展,2016,36(10):957-960.[doi:10.13389/j.cnki.rao.2016.0256]
　HAN Zhi-Hua,YANG Shu-Huan,GUO Wei-Min,et al.Role and significance of TGF-β mediated Smad signaling pathways in proliferative diabetic retinopathy[J].Recent Advances in Ophthalmology,2016,36(10):957-960.[doi:10.13389/j.cnki.rao.2016.0256]
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TGF-β介导的Smad信号通路在增生型糖尿病视网膜病变中的作用和意义
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年10期
页码:
957-960
栏目:
应用研究
出版日期:
2016-10-05

文章信息/Info

Title:
Role and significance of TGF-β mediated Smad signaling pathways in proliferative diabetic retinopathy
作者:
韩治华; 杨淑焕; 郭卫民; 刘喜燕
710003　陕西省西安市，陕西省中医医院眼科（韩治华，杨淑焕，郭卫民）；710003　陕西省西安市，陕西省中医医院脾胃科（刘喜燕）
Author(s):
HAN Zhi-Hua; YANG Shu-Huan ; GUO Wei-Min ;  LIU Xi-Yan
Department of Ophthalmology , Shaanxi Province Hospital of Traditional Chinese Medicine ( HAN Zhi-Hua, YANG Shu-Huan, GUO Wei-Min) , Xi ’ an 710003 . Shaanxi Province , China ; Department of Spleen and Stomach , Shaanxi Province Hospital of Traditional Chinese Medicine ( LIU Xi-Yan) ,Xi ’ an 710003 . Shaanxi Province , China
关键词:
转化生长因子-β; Smad; 增生型糖尿病视网膜病变
Keywords:
transforming growth factor -β ;  Smad;  proliferative diabetic retinopathy
分类号:
Ｒ７７４.１
DOI:
10.13389/j.cnki.rao.2016.0256
文献标志码:
A
摘要:
目的　本研究旨在观察转化生长因子β（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒ-ｂｅｔａ，ＴＧＦ-β）介导的Ｓｍａｄ信号通路在增生型糖尿病视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｄｉａｂｅｔｉｃｒｅｔｉｎｏｐａｔｈｙ，ＰＤＲ）中的作用和意义，进而阐明其可能的发病机制。方法　收集正常人和糖尿病患者的血液标本，将糖尿病患者依据视网膜受累程度分为２组：无糖尿病视网膜病变（ｎｏｎ-ｄｉａｂｅｔｉｃｒｅｔｉ-ｎｏｐａｔｈｙ，ＮＤＲ）组、糖尿病视网膜病变（ｄｉａｂｅｔｉｃｒｅｔｉｎｏｐａｔｈｙ，ＤＲ）组，根据眼底改变又分为非增生型糖尿病视网膜病变（ｎｏｎ-ｐｒｏｌｉｆｅｒａｔｉｖｅｄｉａｂｅｔｉｃｒｅｔｉｎｏｐａｔｈｙ，ＮＰＤＲ）组和增生型糖尿病视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｄｉａｂｅｔｉｃｒｅｔｉｎｏｐａｔｈｙ，ＰＤＲ）组。分别用ＥＬＩＳＡ检测正常人和糖尿病患者血浆中的ＴＧＦ-β１ 和ＴＧＦ-β２，Ｗｅｓｔｅｒｎｂｌｏｔ检测血浆中ＴＧＦ-β１、ＴＧＦ-β２、Ｓｍａｄ３和ｐ-Ｓｍａｄ３的蛋白表达量，比较各组差异。结果　与对照组比较，糖尿病患者各组ＴＧＦ-β１ 和ＴＧＦ-β２分泌量和ＴＧＦ-β１、ＴＧＦ-β２、ｐ-Ｓｍａｄ３的蛋白表达量均明显升高（均为Ｐ＜０．０５）；与ＮＤＲ组比较，ＤＲ各组上述指标均显著增高（均为Ｐ＜０．０５）；ＰＤＲ组上述指标均显著高于ＮＰＤＲ组（均为Ｐ＜０．０５）。结论　ＴＧＦ-β介导的Ｓｍａｄ信号通路参与了ＤＲ的发生，可能是介导ＰＤＲ发生的重要机制。
Abstract:
Objective To observe the role and significance of transforming growth factor-β ( TGF-β) mediated Smad signaling pathways in the proliferative diabetic retinopathy ( PDR) . and illustrate its possible pathogenesis. Methods Diabetic patients were divided into two groups on the basis of the retinal involvement degree : Nondiabetic retinopathy ( NDR) , diabetic retinopathy ( DR) . According to retina changes, DR were divided into two subtypet : Non-proliferative diabetic retinopathy( NPDR) ,proliferative diabetic retinopathy ( PDR) . The blood samples from normal people and diabetic patients were detected to analyze secretion level of TGF-β, and TGF-β2 by ELISA and the protein expression level of TGF- β i , TGF- β2 , Smad3 , p- Smad3 by Western blot in serum. Results Compared with normal group, the secretion and relative protein expression of TGF- β, , TGF- β2 were significantly higher in diabetic patients ( all P < 0. 05 ) . the relative protein expression of p-Smad3 was also clearly higher in diabetic patients ( all P < 0. 05 ) ; Compared with NDR , the results were similar ( all P < 0. 05 ) ; Subgroup analysis showed that the above indexes were significantly higher in PDR than NPDR patients ( all P < 0. 05 ) . Conclusion TGF- β mediated Smad signaling pathways involves in the occurrence of DR .which may be important pathological mechanism in PDR.

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更新日期/Last Update: 2016-10-25