[1]王勇,管怀进.DNA修复基因在年龄相关性白内障患者晶状体皮质中表达的研究[J].眼科新进展,2016,36(4):310-313.[doi:10.13389/j.cnki.rao.2016.0084]
　WANG Yong,GUAN Huai-Jin.Expression of DNA repair genes in lens cortex of agerelated cataract[J].Recent Advances in Ophthalmology,2016,36(4):310-313.[doi:10.13389/j.cnki.rao.2016.0084]
点击复制

DNA修复基因在年龄相关性白内障患者晶状体皮质中表达的研究
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年4期
页码:
310-313
栏目:
实验研究
出版日期:
2016-04-05

文章信息/Info

Title:
Expression of DNA repair genes in lens cortex of agerelated cataract
作者:
王勇; 管怀进
226001　江苏省南通市，南通大学附属医院眼科研究所
Author(s):
WANG Yong; GUAN Huai-Jin
Eye Institute , Affiliated Hospital of Nantong University, Nantong 226001 , Jiangsu Province , China
关键词:
年龄相关性白内障; 晶状体皮质; DNA修复基因
Keywords:
age-related cataract ; lens cortex; DNA repair gene
DOI:
10.13389/j.cnki.rao.2016.0084
文献标志码:
A
摘要:
目的　研究ＤＮＡ修复基因在年龄相关性白内障（ａｇｅ-ｒｅｌａｔｅｄｃａｔａｒａｃｔ，ＡＲＣ）患者晶状体皮质和正常对照晶状体皮质之间的表达差异。方法　使用ＴａｑＭａｎ人类ＤＮＡ修复基因表达芯片板检测年龄和性别匹配的３例ＡＲＣ患者和３例正常对照晶状体皮质组织中ＤＮＡ修复基因的表达。表达差异在１．５倍以上的基因使用实时荧光定量聚合酶链式反应（ｒｅａｌ-ｔｉｍｅＰＣＲ）进行验证（３０例ＡＲＣ患者和３０例正常对照）。数据使用ＳＰＳＳ１７．０软件进行分析，ＡＲＣ患者与正常对照间数据的比较采用独立样本ｔ检验。结果　ＴａｑＭａｎ人类ＤＮＡ修复基因表达芯片板检测显示：相对于正常对照晶状体皮质，在ＡＲＣ患者晶状体皮质中有７个ＤＮＡ修复基因（ＡＴＭ、ＥＲＣＣ６、ＰＯＬＡ１、ＰＯＬＤ１、ＰＯＬＱ、ＰＳＭＢ８、ＣＣＮＯ）表达下调１．５倍以上（０．３５±０．０７、０．２６±０．０９、０．４１±０．０７、０．３７±０．１４、０．３７±０．０７、０．１５±０．０５、０．５７±０．１３），差异均有统计学意义（ｔ＝８．９８，Ｐ＝０．０１；ｔ＝４．７１，Ｐ＝０．０４；ｔ＝１０．４２，Ｐ＝０．０１；ｔ＝４．６５，Ｐ＝０．０４；ｔ＝８．９２，Ｐ＝０．０１；ｔ＝４．９４，Ｐ＝０．０４；ｔ＝７．６３，Ｐ＝０．０２），４个基因（ＣＨＥＫ２、ＥＲＣＣ１、ＦＡＮＣＥ、ＧＡＤＤ４５Ｇ）表达上调１．５倍以上（２．５８±０．２５、１．９５±０．０９、８．８２±０．７８、３．１８±０．８９），差异均有统计学意义（ｔ＝１８．１８，Ｐ＝０．００；ｔ＝２０．９２，Ｐ＝０．０１；ｔ＝１９．５５，Ｐ＝０．０１；ｔ＝６．２０，Ｐ＝０．０２）。ｒｅａｌ-ｔｉｍｅＰＣＲ的验证结果与其一致。结论　ＡＲＣ患者晶状体皮质和正常对照晶状体皮质中部分ＤＮＡ修复基因的表达存在差异，这些表达有差异的基因可能在ＡＲＣ的形成和发展中起到一定作用。
Abstract:
Objective To examine the diffidence expression of DNA repair genes between lens cortex of age-related cataract ( ARC) and controls. Methods Thirty-three ARC patients and thirty-three controls were included in this study. Human DNA Repair Mechanism TaqMan ~ arrays plates were used to examine the DNA repair genes in lens cortex of ARC and controls ( three ARC patients and three controls) . Then the real-time PCR was used to confirm the diffidence expression of genes identified by the arrays plates ( thirty ARC patients and thirty controls). Results In lens cortex of ARC ,7 genes ( ATM . ERCC6 . POLAI , POLDI , POLQ , PSMB8 , CCNO ) had significantly 1. 5 folds lower expression levels ( 0. 35 + 0. 07 , 0. 26 + 0. 09 , 0. 41 + 0. 07 , 0. 37 + 0. 14 . 0. 37 +0. 07 , 0. 15 + 0. 05 ,0. 57 + 0. 13 ) in the ARC group compared with the control group ( t = 8. 98 ,P = 0. 01 ; t = 4. 71 ,P = 0. 04 ; t = 10. 42 ,P = 0. 01 ; t = 4. 65 ,P = 0. 04 ; t = 8. 92 ,P = 0. 01 ; t = 4. 94 ,P = 0. 04; t = 7. 63 ,P = 0. 02 ) and 4 genes ( CHEK2 . ERCCI . FANCE , GADD45G) had significantly l. 5 folds higher expression levels ( 2. 58 + 0. 25 . 1. 95 +0. 09 ,8. 82 +0. 78 .3. 18 +0. 89) ( t = 18. 18 ,P = 0. 00 ; t = 20. 92 .P = 0. 01 ; t = 19. 55 . P = 0. 01 ; t = 6. 20 ,P = 0. 02 ) . The results of real-time PCR were consistent with data of arrays plates. Conclusion There are different expressions of some DNA repair genes between lens cortex of ARC and normal controls. The data may provide evidence that altered expression of DNA repair genes is associated with pathogenesis of ARC.

相似文献/References:

[1]边领斋 刘巨平 魏瑞华 李筱荣 赵少贞 华宁 王铁成 李颖 任新军 梁娟 邹媛媛 赵小云 刘文洁.云南省高海拔农村多民族聚居区年龄相关性白内障危险因素调查[J].眼科新进展,2013,33(1):000.
[2]张虹 胡竹林.超声乳化术对高度近视合并年龄相关性白内障患者泪膜稳定性及泪液蛋白的影响[J].眼科新进展,2013,33(3):000.
[3]江志坚 薛敏 董健鸿 朱茂丽 王敏华.年龄相关性白内障患者角膜内皮细胞特征分析[J].眼科新进展,2013,33(10):000.
[4]徐欢欢,赵长霖,薛春燕,等. 年龄相关性白内障患者角膜Q值的分布及影响因素[J].眼科新进展,2014,34(7):681.[doi:10.13389/j.cnki.rao.2014.0187]
[5]周婧,管怀进. 实时荧光定量PCR技术在年龄相关性白内障患者GSTM1、GSTT1基因拷贝数变异检测中的应用[J].眼科新进展,2014,34(9):838.[doi:10.13389/j.cnki.rao.2014.0231]
[6]赵晓彬,张海芳,董素婷. 年龄相关性白内障角膜前表面高阶像差分析[J].眼科新进展,2015,35(4):359.[doi:10.13389/j.cnki.rao.2015.0096]
　ZHAO Xiao-Bin,ZHANG Hai-Fang,DONG Su-Ting. Analysis of anterior corneal higher-order aberrations in age-related cataract[J].Recent Advances in Ophthalmology,2015,35(4):359.[doi:10.13389/j.cnki.rao.2015.0096]
[7]顾珊珊,戎晗,张国伟,等.单核苷酸多态性与年龄相关性白内障的研究进展[J].眼科新进展,2016,36(7):691.[doi:10.13389/j.cnki.rao.2016.0184]
　GU Shan-Shan,RONG Han,ZHANG Guo-Wei,et al.Progress on single nucleotide polymorphism and agerelated cataract[J].Recent Advances in Ophthalmology,2016,36(4):691.[doi:10.13389/j.cnki.rao.2016.0184]
[8]谭钢,吴安花,邵毅,等.miR-210靶向沉默Bcl-2诱导人晶状体上皮细胞凋亡[J].眼科新进展,2016,36(8):701.[doi:10.13389/j.cnki.rao.2016.0186]
　TAN Gang,WU An-Hua,SHAO Yi,et al.MiR-210 induced human lens epithelial cell apoptosis by target silencing Bcl-2[J].Recent Advances in Ophthalmology,2016,36(4):701.[doi:10.13389/j.cnki.rao.2016.0186]
[9]王勇,杨梅,管怀进.ERCC6基因单核苷酸多态性与年龄相关性白内障的关联研究[J].眼科新进展,2016,36(11):1029.[doi:10.13389/j.cnki.rao.2016.0274]
　WANG Yong,YANG Mei,GUAN Huai-Jin.Association of ERCC6 gene single nucleotide polymorphisms with age-related cataract[J].Recent Advances in Ophthalmology,2016,36(4):1029.[doi:10.13389/j.cnki.rao.2016.0274]
[10]王勇,杨梅,李渤,等.DNA甲基化转移酶（DNMT）3b基因的单核苷酸多态性与年龄相关性白内障[J].眼科新进展,2017,37(1):035.[doi:10.13389/j.cnki.rao.2017.0009]
　WANG Yong,YANG Mei,LI Bo,et al.Association of DNMT3b gene single nucleotide polymorphisms with age-related cataract[J].Recent Advances in Ophthalmology,2017,37(4):035.[doi:10.13389/j.cnki.rao.2017.0009]

备注/Memo

备注/Memo:
国家自然科学基金项目（编号：８１０７０７１８）
更新日期/Last Update: 2016-04-01