[1]周赟,刘宁宁,万超,等.β-榄香烯对糖尿病大鼠视网膜中肿瘤坏死因子-α表达的影响[J].眼科新进展,2015,35(12):1129-1131.[doi:10.13389/j.cnki.rao.2015.0309]
　ZHOU Yun,LIU Ning-Ning,WAN Chao,et al.Effects of β-elemene on expression of TNF-α in retina of diabetic rats[J].Recent Advances in Ophthalmology,2015,35(12):1129-1131.[doi:10.13389/j.cnki.rao.2015.0309]
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β-榄香烯对糖尿病大鼠视网膜中肿瘤坏死因子-α表达的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年12期
页码:
1129-1131
栏目:
实验研究
出版日期:
2015-12-05

文章信息/Info

Title:
Effects of β-elemene on expression of TNF-α in retina of diabetic rats
作者:
周赟; 刘宁宁; 万超; 孙一洲; 陈蕾
110001　辽宁省沈阳市，中国医科大学附属第一医院眼科
Author(s):
ZHOU Yun ;  LIU Ning-Ning; WAN Chao ; SUN Yi-Zhou; CHEN Lei
Department Ophthalmology , the First Affiliated Hospital of China Medical University , Shenyang 110001 , Liaoning Province . China
关键词:
β-榄香烯; 糖尿病大鼠; 肿瘤坏死因子-α
Keywords:
β-elemene ;  diabetic rat ;  TNF-α
DOI:
10.13389/j.cnki.rao.2015.0309
文献标志码:
A
摘要:
目的　探讨β-榄香烯对糖尿病大鼠视网膜肿瘤坏死因子-α（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ-α，ＴＮＦ-α）表达的影响。方法　１０周龄ＳＤ大鼠３６只，随机分为６组，除Ａ组（每只大鼠腹腔注射等量的柠檬酸钠缓冲液）外其余组腹腔注射链脲佐菌素（ｓｔｒｅｐｔｏｚｏｔｏｃｉｎ，ＳＴＺ）制成糖尿病大鼠模型，成模６周后，Ｃ组每只大鼠双眼球周给予空白乳剂５μＬ，Ｄ组双眼球周给予β-榄香烯５μＬ，Ｅ组每只大鼠同时双眼球内给予空白乳剂５μＬ，Ｆ组双眼球内给予β-榄香烯５μＬ，Ｂ组未采取局部注射作为对照。末次给药４８ｈ后处死大鼠，取眼球固定视网膜行免疫组织化学法检测视网膜中ＴＮＦ-α的表达情况；提取视网膜蛋白，采用ＷｅｓｔｅｒｎＢｌｏｔ检测ＴＮＦ-α蛋白的表达情况。结果　免疫组织化学法检测结果显示：Ａ组视网膜未见阳性染色，Ｂ组视网膜全层可见阳性表达，Ｃ组及Ｅ组阳性表达分布与Ｂ组相同，而Ｄ组及Ｆ组棕色阳性表达下降，其中Ｆ组更显著。ＷｅｓｔｅｒｎＢｌｏｔ检测结果显示：Ｃ组及Ｅ组ＴＮＦ-α蛋白的表达与Ａ组相比差异均无统计学意义（均为Ｐ＞０．０５），Ｂ组较Ａ组增高（Ｐ＜０．０１），而Ｄ组及Ｆ组表达与Ｂ组差异均有统计学意义（均为Ｐ＜０．０５）。结论　β-榄香烯球周及球内注射均可降低糖尿病大鼠视网膜内ＴＮＦ-α的表达，且眼内注射效果更好。
Abstract:
Objective To investigate the effects of β-elemene on the expression of TNF-α in the retina of diabetic rats. Methods Thirty-six lO-week-old Sprague-Dawley rats were randomly divided into control group ( group A) . diabetic rats group ( group B) ,periocular compared group ( group C) ,periocular treating group ( group D) , intraocular compared group ( group E) ,intraocular treating group ( group F) . Group B, C , D . E were induced the diabetic model. Then , after 6 weeks of diabetic course , group C and D were treated with blank emulsion and β-elemene periocular injections ,respectively ; Meanwhile , group E and F were treated with blank emulsion and β-elemene intraocular injections ,respectively. Then the retinas were fixed and immunohistochemical method was used to observe TNF-α expression in the retinas. Western Blot was used to measure the difference of TNF-α protein expression. Results Immunohistochemical results showed that no positive stairung was seen in retina of group A,but seen in whole layer of group B , group C and E were same as group B, and the positive expression in group D and F decreased,especially group F. Western Blot showed that there was no statistical difference in TNF-α protein expression between group A and group C , group A and group E ( all P > 0. 05 ) , group B was higher than group A (P < 0. 01 ) . there were statistical differences between group B and group D, group B and group F ( all P < 0. 05 ) . Conclusion β-elemene injected both periocularly and intraocularly can down-regulate the expression of TNF-α in the retina of diabetic rats , and the effect of intraocular injection is better.

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[2]耿金 陈蕾 柳力敏 刘宁宁 刘磊.β-榄香烯抑制氧诱导小鼠视网膜新生血管形成的实验研究[J].眼科新进展,2013,33(3):000.
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更新日期/Last Update: 2015-12-07