[1]汪伟,李翔,王桃,等. 补肾活血中药对大鼠慢性高眼压模型视网膜神经节细胞PI3K/Akt信号转导通路p-Akt表达的影响[J].眼科新进展,2015,35(9):816-820.[doi:10.13389/j.cnki.rao.2015.0223]
　WANG Wei,LI Xiang,WANG Tao,et al. Effects of Bushenhuoxue on expression of p-Akt of PI3K/Akt sigruid transduction pathway in RGC in rat model of chronic elevated intraocular pressure[J].Recent Advances in Ophthalmology,2015,35(9):816-820.[doi:10.13389/j.cnki.rao.2015.0223]
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 补肾活血中药对大鼠慢性高眼压模型视网膜神经节细胞PI3K/Akt信号转导通路p-Akt表达的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年9期
页码:
816-820
栏目:
实验研究
出版日期:
2015-09-05

文章信息/Info

Title:
 Effects of Bushenhuoxue on expression of p-Akt of PI3K/Akt sigruid transduction pathway in RGC in rat model of chronic elevated intraocular pressure
作者:
 汪伟; 李翔; 王桃; 柯欣怡; 刘红佶; 贾正品; 牟琳
 610072　四川省成都市，成都中医药大学
Author(s):
 WANG Wei ;  LI Xiang; WANG Tao ;  KE Xin-Yi;  LIU Hong-Ji;  JIA Zheng-Pin; MOU Lin
 Chengdu University of TCM, Chengdu 610072 , Sichuan Province. China
关键词:
 ; 补肾活血中药; 视网膜神经节细胞; PI3K/Akt信号通路; p-Akt; 青光眼; 大鼠慢性高眼压模型
Keywords:
 ; Bushenhuoxue ;  retinal ganglion cells ;  PI3K/Akt signal pathway ;  p-Akt ;  glaucoma ;  rat chronic elevated intraocular pressure model
DOI:
10.13389/j.cnki.rao.2015.0223
文献标志码:
A
摘要:
 目的　观察补肾活血中药对大鼠慢性高眼压（ｅｌｅｖａｔｅｄｉｎｔｒａｏｃｕｌａｒｐｒｅｓｓｕｒｅ，ＥＩＯＰ）模型视网膜神经节细胞（ｒｅｔｉｎａｌｇａｎｇｌｉｏｎｃｅｌｌｓ，ＲＧＣ）ＰＩ３Ｋ／Ａｋｔ信号转导通路ｐ-Ａｋｔ表达的影响。方法　３０只ＳＤ大鼠随机分成３组：对照组、模型组和给药组，模型组和给药组建立ＥＩＯＰ模型。给药组造模后每天予复方丹参片和杞菊地黄丸的混悬液灌胃，每天１次，连续８周，对照组、模型组每天予生理盐水灌胃。记录造模前、造模后即刻、造模后８周的眼压；光镜下观察各组视网膜厚度和ＲＧＣ数量，电镜下观察视网膜超微结构，免疫组织化学法检测视网膜内ｐ-Ａｋｔ的表达。结果　造模后８周眼压与造模后即刻相比，给药组有降低趋势，差异有统计学意义（Ｐ＜０．０１）。造模后８周，给药组与模型组ＲＧＣ数量均低于对照组，差异均有统计学意义（均为Ｐ＜０．０５）；对照组视网膜厚度与给药组相比，差异无统计学意义（Ｐ＞０．０５），而对照组与模型组相比，差异有统计学意义（Ｐ＜０．０１）；给药组ＲＧＣ数量及视网膜厚度均高于模型组，差异均有统计学意义（均为Ｐ＜０．０１）。电镜下ＲＧＣ超微结构显示，模型组损伤明显，给药组较模型组有所改善。视网膜ｐ-Ａｋｔ表达免疫组织化学结果分析显示，给药组ｐ-Ａｋｔ阳性染色总面积、平均光密度、积分光密度值与模型组相比，差异均有统计学意义（均为Ｐ＜０．０１），给药组黑度（１３９．９７±１１．７９）虽高于模型组（１３７．９５±６．１３），但差异无统计学意义（Ｐ＞０．０５）。结论　补肾活血中药用于ＳＤ大鼠慢性ＥＩＯＰ模型，能降低眼压，提高ＲＧＣ数量及改善超微结构，增加视网膜厚度，上调ＰＩ３Ｋ／Ａｋｔ信号转导通路中ｐ-Ａｋｔ的表达。
Abstract:
 Objective To observe the effects of Bushenhuoxue decoction on expression of p-Akt of PI3K/Akt signal transduction pathway in retinal ganglion cells ( RGC) in rat model of chronic elevated intraocular pressure ( EIOP) . Methods Thirty SD rats were randomly divided int0 3 groups : control group , model group , treatment group, and EIOP models were established in meodel group and drug group. After the models established.the rats in treatment group were given the intragastric administration of Bushenhuoxue decoction. once per day for 8 weeks , and the control group and model group were grven normal sodium. The intraocular pressure ( IOP) before modeling and immediately,8 weeks after modeling were recorded. The number of RGC and retinal thickness were observed under the light microscope, the retinal ultrastructure was observed under the electron microscope , and the expression of p-Akt in retina was detected by immunohistochemistry. Results Compared with immediately after modeling ,IOP in treatment group at 8 weeks after modeling decreased,the difference was statistically significant ( P < 0. 01 ) . At 8 weeks after modeling, RGC number in treatment group and model group were lower than that in control group ,the differences were statistically significant ( all P < 0. 05 ) ; The difference in retinal thickness between control group and treatment group was not statistically significant (P > 0. 05 ) , but the difference between control group and model group was statistically significant ( P < 0. 01 ) ; RGC number and retinal thickness in treatment group were better than those in model group , the differences were statistically significant ( all P < 0. 01 ) . Under the electron microscope , the RGC ultrastructure injured obviously in model group , and the treatment group was improved. Immunohistochemical analysis of p-Akt expression in the retina showed that there were statistical differences in the total area, average optical density , integral optical density of p-Akt positive stairung between treatment group and model group ( all P < 0. 01 ) , and the average density of treatment group ( 139. 97 + 11. 79) was higher than that of model group ( 137. 95 + 6. 13 ) , but the difference was not statistically sigruficant ( P > 0. 05 ) . Conclusion Bushenhuoxue for chronic EIOP SD rat model can reduce intraocular pressure ,increase the RGC quantity and improve the ultrastructure ,increase retinal thickness , up-regulate p-Akt expression of PI3K/Akt signal transduction pathway.

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备注/Memo

备注/Memo:
 国家自然科学基金资助（编号：８１３７３６９５）
更新日期/Last Update: 2015-08-31