[1]孙晓伟,殷晓贝,崔卉,等. 阿魏酸对视网膜色素变性小鼠血浆内皮素-1表达的影响[J].眼科新进展,2015,35(8):701-703.[doi:10.13389/j.cnki.rao.2015.0192]
　SUN Xiao-Wei,YIN Xiao-Bei,CUI Hui,et al. Effects of ferulic acid on serum endothelin-l of RP mouse[J].Recent Advances in Ophthalmology,2015,35(8):701-703.[doi:10.13389/j.cnki.rao.2015.0192]
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 阿魏酸对视网膜色素变性小鼠血浆内皮素-1表达的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年8期
页码:
701-703
栏目:
实验研究
出版日期:
2015-08-05

文章信息/Info

Title:
 Effects of ferulic acid on serum endothelin-l of RP mouse
作者:
 孙晓伟; 殷晓贝; 崔卉; 李根林
 100730　北京市首都医科大学附属北京同仁医院北京同仁眼科中心　北京市眼科学与视觉科学重点实验室
Author(s):
 SUN Xiao-Wei; YIN Xiao-Bei ;  CUI Hui;  LI Gen-Lin
 Beijing Tongren Eye Center, Beijing Tongren Hospital,Capital Medical University,Beijing Ophthalmology & Visual Sciences Key Lab, Beijing 100730，China
关键词:
 ; 阿魏酸; 视网膜色素变性; 内皮素-1; rd小鼠
Keywords:
 ; ferulic acid ;  retinitis pigmentosa ;  endothelin-l ;  rd mouse
DOI:
10.13389/j.cnki.rao.2015.0192
文献标志码:
A
摘要:
 目的　研究不同浓度阿魏酸作用下，出生后不同时期的视网膜色素变性（ｒｅｔｉｎｉｔｉｓｐｉｇｍｅｎｔｏｓａ，ＲＰ）动物模型（ｒｄ小鼠）血浆中内皮素－１（ｅｎｄｏｔｈｅｌｉｎ－１，ＥＴ－１）动态表达特征，探讨阿魏酸对ｒｄ小鼠表达ＥＴ－１的作用特点。方法　取新出生的ｒｄ小鼠９０只及Ｃ５７／ＢＬ６小鼠１８只，按照阿魏酸灌胃浓度的不同分成４组，０．２５ｇ? Ｌ－１组、０．５０ｇ? Ｌ－１组、０．７５ｇ?Ｌ－１组、１．００ｇ?Ｌ－１组，每组再按照给药时间分为２周组、３周组、４周组，以上１２组为药物处理组，每组各６只。相同周龄的ｒｄ小鼠各６只不作处理作为病变对照组，相同周龄的Ｃ５７／ＢＬ６小鼠各６只作为正常对照组。ＥＬＩＳＡ法检测血浆ＥＴ－１浓度。结果　２周组、３周组、４周组病变对照组小鼠ＥＴ－１浓度均高于正常对照组，尤其３周组、４周组与正常对照组相比差异有统计学意义（Ｐ＝０．０２４、０．０１０）。经阿魏酸治疗后２周时，０．５０ｇ?Ｌ－１组、０．７５ｇ?Ｌ－１组ＥＴ－１水平低于病变对照组，但差异无统计学意义；３周时，０．２５ｇ?Ｌ－１组、０．５０ｇ?Ｌ－１组、０．７５ｇ?Ｌ－１组ＥＴ－１水平低于病变对照组，其中０．５０ｇ?Ｌ－１组差异有统计学意义（Ｐ＝０．０３４）；４周时，０．２５ｇ?Ｌ－１组、０．５０ｇ?Ｌ－１组、０．７５ｇ?Ｌ－１组ＥＴ－１水平低于病变对照组，差异均有统计学意义（Ｐ＝０．０１１、０．０２７、０．０２１）。各浓度治疗组中，仅１．００ｇ?Ｌ－１组治疗３周和４周时与正常对照组相比差异有统计学意义（Ｐ＝０．０１３、０．００９）。结论　ｒｄ小鼠在病变过程中，血浆ＥＴ－１水平明显升高，可能与ＲＰ的发生发展有关。在不同浓度阿魏酸治疗下，ｒｄ小鼠ＥＴ－１水平不同程度下降，其中０．５０ｇ?Ｌ－１、０．７５ｇ?Ｌ－１治疗效果最明显。
Abstract:
 Objective To investigate the effects of ferulic acid on the serum endothelin-l of rd mouse following gavage administration of ferulic acid ( FA) . Methods A total of 90 newborn rd mice were divided into the control group and four gavage administration groups (0. 25 g . L-l group, 0. 50 g . L-l group, 0. 75 g . L-l group and 1. 00 g . L-l group) , and 18 C57/BL6 mice were included in the normal control group. The gavage adnunistration groups were divided int0 2-week group , 3-week group and 4-week group based on the gavage administration time.6 cases in each group , and ELISA was performed to detect ET-I in serum. Results ET-I levels in 2-week group, 3-week group and 4-week group were significantly higher than that in normal control group , especially the 2-week group , 3-week group ( P = 0. 024 , 0. 010 ) . After treated with different concentrations of ferulic acid for 2 weeks. ET-I levels in 0. 50 g . L -l group, 0. 75 g . L -l group were lower than that in diseased control group ; After treated for 3 weeks,ET-I levels in 0. 25 g . L-l group, 0. 50 g . L-l group,0. 75 g . L-l group were lower than that in diseased control group, and there was statistical difference in 0. 50 g . L -l group (P = 0. 034 ) ;After treated for 4 weeks , ET-I levels in 0. 25 g . L -l group , 0. 50 g . L-l group ,0. 75 g . L-l group were lower than that in diseased control group, and there were statistical difference (P = 0. 011 , 0. 027 .0. 021 ) . There were statistical differences in ET-I levels in l. 00 g . L-l group for 3 weeks and 4 weeks compared with normal control group ( P = 0. 013 .0. 009) . Coriclrision ET-I levels in mice plasma rncrease significantly in the process of pathological changes of rd mouse , and may be associated with the development of RP. Under the different concentrations of ferulic acid treatment . ET-I levels significantly decrease. The effects are obvious in 0. 50 g . L -1 and 0. 75 g . L-1 concentrations.

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备注/Memo

备注/Memo:
 国家自然科学基金资助（编号：８１２７１０４６）；北京市教委科研计划重点项目（编号：ＫＺ２０１５１００２５０２５）
更新日期/Last Update: 2015-07-31