[1]刘太祥,王铮. 京尼平诱导兔眼巩膜胶原交联的有效性和安全性[J].眼科新进展,2015,35(7):619-622.[doi:10.13389/j.cnki.rao.2015.0168]
　LIU Tai-Xiang,WANG Zheng. Efficiency and safety of crosslinking of scleral collagen using genipin in rabbit[J].Recent Advances in Ophthalmology,2015,35(7):619-622.[doi:10.13389/j.cnki.rao.2015.0168]
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 京尼平诱导兔眼巩膜胶原交联的有效性和安全性
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年7期
页码:
619-622
栏目:
实验研究
出版日期:
2015-07-05

文章信息/Info

Title:
 Efficiency and safety of crosslinking of scleral collagen using genipin in rabbit
作者:
 刘太祥; 王铮
 563003　贵州省遵义市，遵义医学院附属医院近视白内障中心
Author(s):
 LIU Tai-Xiang ;  WANG Zheng
 Ophthalmic Centre ,the Affiliated Hospital of Zunyi Medical College, zunyi 563003 , Guizhou Province , China
关键词:
 ; 巩膜; 胶原交联; 生物力学; 近视
Keywords:
 ; sclera ;  collagen cross-linking ;  biomechanics ;  myopia
DOI:
10.13389/j.cnki.rao.2015.0168
文献标志码:
A
摘要:
 目的　探索京尼平诱导兔眼巩膜胶原交联的有效性和安全性，为防治近视进展寻找有效的方法。方法　２０只成年新西兰大白兔，按简单数字随机方法选一眼Ｔｅｎｏｎ’ｓ囊下注射０．５ｍｍｏｌ?Ｌ－１京尼平０．５ｍＬ２次和４次各１０只，再按简单数字随机选对侧眼１０只为对照眼；每组５只测试生物力学，另５只进行组织学检查。结果　与对照眼比较，Ｔｅｎｏｎ’ｓ囊下注射２次和４次，巩膜的极限应力分别增加了９５％和１６１％；同时２０％应变时弹性模量分别增加了２０４％和２６０％，而极限应变则分别下降了１３％和２２％。京尼平没有引起巩膜、脉络膜和视网膜中的细胞凋亡，也没有损伤邻近组织角膜和视神经，治疗区域没有明显的颜色变化。结论　京尼平诱导兔眼巩膜胶原交联安全有效，是一种潜在的防治近视进展的方法。
Abstract:
 Objective To evaluate the efficiency and safety of crosslinking scleral collagen using genipin in rabbit . and develop new therapy for preventing the progression of myopia. Methods Twenty New Zeal rabbits were randomly divided into two groups (n = 10) for sub-Tenon ’ s injection of 0. 5 mmol . L-l genipin for 2 times and 4 times . and the contralateral eyes were randomly selected 10 eyes as control. Five eyes in each group were for stress-strain measurements .and other 5 eyes were prepared for histological examination. Results Compared with untreated sclera strips , after sub-Tenon injecting with genipin for 2 times and 4 times. the ultimate stress of genipin crosslinked sclera increased by an amplitude of 95% and 161% . The Young ’ s modulus at 20% strain increased by 204% and 260% , respectively. And ultimate strain decreased by 13% and 22% . It did not cause apoptosis in scleral , choroid and retinal cells ; and no side effects were found in the peripheral cornea and optic nerve. There had no visible discoloration around treated area. Conclusion Genipin-induced collagen crosslinking is effective and safe in rabbit , and is a potential agent to strengthen the weaken sclera to prevent progressive myopia.

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备注/Memo

备注/Memo:
 广东省科技计划项目基金资助（编号：２００７Ｂ０３１００２００１）
更新日期/Last Update: 2015-07-03