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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:

35卷

期数:

2015年6期

页码:

528-531

栏目:

实验研究

出版日期:

2015-06-05

文章信息/Info

Title:

 Effects of Carboplatin combined with MicroRNA-34a on inhibition of HXO-RB44 cells proliferation and tumor growth

作者:

 胡辅华; 刘丽林; 季建; 于金国

 300456　天津市，天津港口医院眼科

Author(s):

 HU Fu-Hua ;  LIU Li-Lin ; JI Jian; YU Jin-Guo

 Department of Ophthalmology, Tianjin Port Hospital, Tianjin 300456 , China

关键词:

 ; 卡铂; MicroRNA-34a; 视网膜母细胞瘤

Keywords:

 ; Carboplatin ;  MicroRNA-34a ;  retinoblastoma

DOI:

10.13389/j.cnki.rao.2015.0143

文献标志码:

A

摘要:

 目的　探讨卡铂联合ＭｉｃｒｏＲＮＡ-３４ａ对视网膜母细胞瘤ＨＸＯ-ＲＢ４４细胞的增殖抑制作用和对裸鼠异位瘤的生长抑制作用。方法　将ＨＸＯ-ＲＢ４４细胞分成卡铂干预组、ＭｉｃｒｏＲＮＡ-３４ａ干预组、联合干预组和生理盐水对照组。ＭＴＴ检测卡铂联合ＭｉｃｒｏＲＮＡ-３４ａ干预对ＨＸＯ-ＲＢ４４细胞的毒性。流式细胞技术检测卡铂联合ＭｉｃｒｏＲＮＡ-３４ａ干预诱导ＨＸＯ-ＲＢ４４细胞凋亡和对细胞周期的影响。构建裸鼠视网膜母细胞瘤异位肿瘤模型，检测卡铂联合ＭｉｃｒｏＲＮＡ-３４ａ干预对裸鼠肿瘤的生长抑制能力。结果　给药４８ｈ后，ＨＸＯ-ＲＢ４４细胞的存活率：分别为卡铂干预组（５８．５±４．４）％、ＭｉｃｒｏＲＮＡ-３４ａ干预组（６４．３±３.１）％、联合干预组（２７．８±２．２）％，差异有统计学意义（Ｐ＜０．０１）；ＨＸＯ-ＲＢ４４细胞的凋亡率：分别为卡铂干预组（１３．５±１.４）％、ＭｉｃｒｏＲＮＡ-３４ａ干预组（８．２±１．１）％、联合干预组（３１．５±２．２）％，差异有统计学意义（Ｐ＜０．０１）；联合干预组较生理盐水对照组Ｓ期细胞增加，Ｇ０／Ｇ１期细胞减少，Ｇ２／Ｍ期细胞减少（均为Ｐ＜０．０１）；荷瘤裸鼠治疗实验结果显示卡铂干预组、ＭｉｃｒｏＲＮＡ-３４ａ干预组和联合干预组的肿瘤生长抑制率分别为４４．６％ ±１．３％、３６．５％ ±１．３％和７５．３％ ±１．５％，与生理盐水对照组差异均有统计学意义（均为Ｐ＜０．０１）。结论　卡铂联合ＭｉｃｒｏＲＮＡ-３４ａ能够有效抑制ＨＸＯ-ＲＢ４４细胞的增殖和肿瘤的生长。

Abstract:

 Objective To evaluate the effects of Carboplatin combined with MicroRNA-34a on the inhibition of HXO-RB44 cells proliferation and tumor growth. Methods The HXO-RB44 cells were divided into Carboplatin group , MicroRNA-34a group , Carboplatin + MicroRNA-34a group and saline group. The MTT assay was used to evaluate the anti-proliferation efficiency of Carboplatin combined with MicroRNA-34a. Flow cytometry was used to evaluate the HXO-RB44 cells induced apoptosis in Carboplatin + MicroRNA-34a group. HXO-RB44 cells were xenografted in mice to establish the animal model, which were used to evaluate the effect of anti-tumor. Results The cell viability of HXO-RB44 cells in Carboplatin group , MicroRNA-34a group and Carboplatin + MicroRNA-34a group were ( 58. 5 + 5. 2 ) % , ( 64. 3 + 7. I ) % . ( 27. 8 + 3. 2 ) % , respectively , there was significant difference (P < 0. 01 ). The apoptotic rate of HXO-RB44 cells in Carboplatin group, MicroRNA-34a group and Carboplatin + MicroRNA-34a group were ( 13. 5 + 1. 4 ) % , ( 8. 2 + 1. 1) % . ( 31. 5 +2. 2) % , respectively , there was sigruficant difference (P < 0. 01) . Compared with the saline group , the cells in S phase in Carboplatin + MicroRNA-34a group increased , the cells in GO/GI phase decreased, and the cells in G2/ M phase also decreased ( all P < 0. 01 ) . The inhibitive rate of tumor growth in Carboplatin group , MicroRNA-34a group and Carboplatin + MicroRNA-34a group were ( 44. 6 + 1. 3 ) % . ( 36. 5 + 1. 3 ) % , ( 75. 3 + 1. 5 ) % , respectively , there was significant difference compared with the saline group ( all P < 0. 01 ) . Conclusion Carboplatin combined with MicroRNA-34a can effectively inhibit the HXO-RB44 cells proliferation and tumor growth.

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更新日期/Last Update: 2015-06-01