[1]马岚,张奕霞,练海东,等. 右美沙芬对糖尿病视网膜神经病变中Caspase-3表达的影响[J].眼科新进展,2015,35(5):432-434.[doi:10.13389/j.cnki.rao.2015.0117]
　MA Lan,ZHANG Yi-Xia,LIAN Hai-Dong,et al. Effects of dextromethorphan on expression of Caspase-3 in retinal nerve cell of rats with diabetic retinopath[J].Recent Advances in Ophthalmology,2015,35(5):432-434.[doi:10.13389/j.cnki.rao.2015.0117]
点击复制

 右美沙芬对糖尿病视网膜神经病变中Caspase-3表达的影响
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年5期
页码:
432-434
栏目:
实验研究
出版日期:
2015-05-05

文章信息/Info

Title:
 Effects of dextromethorphan on expression of Caspase-3 in retinal nerve cell of rats with diabetic retinopath
作者:
 马岚; 张奕霞; 练海东; 郝小英
 832000　新疆维吾尔自治区石河子市，石河子大学医学院
Author(s):
 MA Lan; ZHANG Yi-Xia ;  LIAN Hai-Dong ;  HAO Xiao-Ying
 Medical College of Shihezi University, Shihezi 832000 , Xinjiang Uygur Autonomous Region , China
关键词:
 ; 糖尿病视网膜病变; 右美沙芬; Caspase-3; 凋亡; 鼠
Keywords:
 ; diabetic retinopathy ;  dextromethorphan ;  Caspase-3 ;  apoptosis ;  rat
DOI:
10.13389/j.cnki.rao.2015.0117
文献标志码:
A
摘要:
 目的　观察右美沙芬对糖尿病大鼠视网膜神经病变中Ｃａｓｐａｓｅ-３表达的影响。方法　健康成年ＳｐｒａｇｕｅＤａｗｌｅｙ大鼠４８只，１２只作为正常对照组（Ｄ组），３６只大鼠链脲佐菌素腹腔注射建立糖尿病大鼠模型，并随机分为糖尿病模型组（Ａ组），１０ｍｇ?ｋｇ－１右美沙芬治疗组（Ｂ组；造模成功后一次性腹腔注射１０ｍｇ?ｋｇ－１右美沙芬），３０ｍｇ?ｋｇ－１右美沙芬治疗组（Ｃ组；造模成功后一次性腹腔注射３０ｍｇ?ｋｇ－１右美沙芬），每组各１２只。各组分别于４周末、８周末各处死大鼠６只，行免疫组织化学法检测视网膜组织Ｃａｓｐａｓｅ-３蛋白的表达。结果　视网膜Ｃａｓｐａｓｅ-３光密度值：Ａ组４周、８周时分别为０．２５６±０．０３１和０．３７４±０．２３４，Ｂ组分别为０．２４４±０．１２２和０．３５２±０.１５２，Ｃ组分别为０.１７４±０.０２１和０．２５６±０．１３８，Ｄ组分别为０．０７４±０．０１８和０．０７２±０．２３３。Ａ组４周Ｃａｓｐａｓｅ-３蛋白表达主要见于神经节细胞层，８周时阳性表达进一步增加，扩展到内核层。Ｃ组Ｃａｓｐａｓｅ-３表达变化规律与Ａ组基本一致，但４周、８周时阳性表达量均低于Ａ组（均为Ｐ＜０．０５），Ａ组与Ｂ组间４周和８周时Ｃａｓｐａｓｅ-３阳性表达量差异均无统计学意义（均为Ｐ＞０．０５）。Ｄ组４周、８周时大鼠视网膜上几乎无Ｃａｓｐａｓｅ-３阳性表达。结论　早期糖尿病大鼠视网膜Ｃａｓｐａｓｅ-３参与了糖尿病视网膜病变的发病机制，右美沙芬可以抑制Ｃａｓｐａｓｅ-３蛋白的表达，对糖尿病视网膜病变有一定的治疗作用。
Abstract:
 Objective To observe the effect of dextromethorphan on the expression of Caspase-3 in retinal nerve cell of rats with diabetic retinopathy. Methods Forty-eight adult Sprague Dawley rats were randomly divided into normal control group( 12 cases , group D) and diabetic model group ( 36 cases were intraperitoneally injected with streptozotocin) . Thirty-six rats were successfully made into diabetic models and randomly divided into diabetes mellitus group ( group A) , 10 mg . kg - ] dextromethorphan group ( group B) and 30 mg . kg -l Dextromethorphan group ( group C) , 12 cases in each group. The six rats of every group were sacrificed at 4 weeks and 8 weeks , the expression of Caspase-3 in retina was studied by immunohistochemistry. Results Average optical density value of Caspase-3 in retina of A group at 4 weeks and 8 weeks were 0. 256 +0. 031 and 0. 374 +0. 234 , respectively , which in group B were 0. 244 + 0. 122 and 0. 352 + 0. 152 , respectively , group C were 0. 174 + 0. 021 and 0. 256 + 0. 138 , respectively , group D were 0. 074 + 0. 018 and 0. 072 + 0. 233 , respectively. At 4 weeks of group A, the positive expression of Caspase-3 was mainly located in ganglion cell layer , and the positive expression was increased and extended to inner nuclear layer at 8 weeks. The trend of Caspase-3 expression was consistent between group C and group A,but the positive expression at 4 weeks and 8 weeks in group C were less than those in group A ( all P < 0. 05 ) . There was no significant difference in Caspase-3 positive expression at 4 weeks and 8 weeks between group A and B ( all P > 0. 05 ) . Positive expression of Caspase-3 was almostly not found in retina of group D at 4 weeks and 8 weeks. Conclusion In early diabetic rats, Caspase-3 plays a role in the development of diabetic retinopathy. Dextromethorphan can inhibit the expression of Caspase-3 ,which has certain therapeutic effect on diabetic retinopathy.

相似文献/References:

[1]杜玮 刘子扬 周艳艳 雒雷鸣.糖尿病视网膜病变与血清胆红素水平的关系[J].眼科新进展,2012,32(5):000.
[2]范松涛 卢建民.阿司匹林与糖尿病患者玻璃体出血以及玻璃体切割术疗效的相关性研究[J].眼科新进展,2012,32(11):000.
[3]李艳 李筱荣 袁佳琴 潘斌.糖尿病大鼠视网膜中VEGF、PEDF的表达与血-视网膜屏障损伤[J].眼科新进展,2013,33(1):000.
[4]李朝晖 崔治华 胡晓英 孟丽珠 张敬维.糖尿病视网膜病变激光面积与疗效的分析[J].眼科新进展,2013,33(2):000.
[5]冯冬梅 朱鸿 施彩虹.CXC趋化因子及其受体在糖尿病视网膜病变中的作用[J].眼科新进展,2013,33(6):000.
[6]牛淑玲.糖尿病视网膜病变患者HbAlc、FPG与血小板参数的变化及危险因素分析[J].眼科新进展,2013,33(7):000.
[7]毕春潮 王睿 王建洲 雷春灵 董晓娟 王小莉 薛晓辉.Ad-PEDF对糖尿病视网膜病变大鼠视网膜新生血管的抑制作用[J].眼科新进展,2013,33(8):000.
[8]杨萍 孙书明 李晓鹏.辛伐他汀对糖尿病视网膜病变和炎症因子的影响[J].眼科新进展,2013,33(8):000.
[9]罗文婷 孙大卫.血管黏附蛋白-1在眼科疾病中的研究进展[J].眼科新进展,2013,33(8):000.
[10]李小璐 马雅玲.糖尿病视网膜病变大鼠视网膜VEGF和PEDF的动态表达[J].眼科新进展,2013,33(9):000.
[11]李琴 谢婷玉 陈雪艺 王清 郭乔茜 具尔提·哈第尔. 臭氧灌肠改善糖尿病大鼠的视网膜电图表现[J].眼科新进展,2014,34(1):034.
[12]蔡晶晶 孟倩丽 郭海科 张良 李东风 崔颖. 血红素加氧酶-1在糖尿病视网膜病变患者外周血单个核细胞中的表达[J].眼科新进展,2014,34(1):037.
[13]张立新 崔颖 孟倩丽 张敏 罗中伶 刘辉焜 朱国平 香淦媚 刘清洋 郭海科 郑洋 蔡晶晶. 广东省东莞市2型糖尿病患者盲与低视力患病率及原因分析[J].眼科新进展,2014,34(1):046.
[14]杨宏伟 韩丁 陈晓隆. 越橘提取物对非增生性糖尿病视网膜病变患者对比敏感度的影响[J].眼科新进展,2014,34(1):065.
[15]徐玲 周小煦 苏畅 吴建国. 超声波提高大鼠视网膜血管消化铺片的效率[J].眼科新进展,2014,34(3):226.
[16]王清 郭乔茜 李琴 谢婷玉 陈雪艺. 臭氧治疗对糖尿病大鼠视网膜细胞凋亡和VEGF、PEDF因子表达的影响[J].眼科新进展,2014,34(3):232.
[17]薛尚才 李惠荣 范勇 温玉 孙建玲. 糖尿病视网膜病变眼轴轴长与中心视网膜厚度的相关性研究[J].眼科新进展,2014,34(3):253.
[18]张敏 崔颖 孟倩丽 刘清洋 张立新 郭海科. 广东省东莞市糖尿病视网膜病变流行病学调查[J].眼科新进展,2014,34(3):260.
[19]陈素芳 曾莉 邵毅. 非诺贝特在糖尿病视网膜病变中的应用进展[J].眼科新进展,2014,34(3):283.
[20]张笃贞,张涤,孙鹏,等. 白藜芦醇对糖尿病大鼠视网膜中SIRT1基因表达的干预作用[J].眼科新进展,2014,34(4):322.[doi:10.13389/j.cnki.rao.2014.0087]

更新日期/Last Update: 2015-04-27