[1]葛佳佳,苏胜,刘平. 地塞米松对人晶状体上皮细胞中波形蛋白表达的影响[J].眼科新进展,2015,35(5):413-415.[doi:10.13389/j.cnki.rao.2015.0112]
　GE Jia-Jia,SU Sheng,LIU Ping. Effects of dexamethasone on vimentin in human lens epithelial cells[J].Recent Advances in Ophthalmology,2015,35(5):413-415.[doi:10.13389/j.cnki.rao.2015.0112]
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 地塞米松对人晶状体上皮细胞中波形蛋白表达的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年5期
页码:
413-415
栏目:
实验研究
出版日期:
2015-05-05

文章信息/Info

Title:
 Effects of dexamethasone on vimentin in human lens epithelial cells
作者:
 葛佳佳; 苏胜; 刘平
 150001　黑龙江省哈尔滨市，哈尔滨医科大学附属第一医院眼科医院
Author(s):
 GE Jia-Jia; SU Sheng; LIU Ping
 Eye Hospital,the First Affiliated Hospital of Harbin Medical Univers ity,Harbin 150001.Heilongjiang Province. China
关键词:
 ; 地塞米松; 波形蛋白; 激素性白内障; 晶状体上皮细胞
Keywords:
 ; dexamethasone ;  vimentin ;  steroid-induced cataract;  human lens epithelial cell
DOI:
10.13389/j.cnki.rao.2015.0112
文献标志码:
A
摘要:
 目的　探讨地塞米松（ｄｅｘａｍｅｔｈａｓｏｎｅ，Ｄｅｘ）对人晶状体上皮细胞系（ｈｕｍａｎｌｅｎｓｅｐｉｔｈｅｌｉａｌｃｅｌｌｓ，ＨＬＥＣ）中波形蛋白表达的影响以及波形蛋白表达变化在糖皮质激素性白内障形成中的作用。方法　对ＨＬＥＣ进行传代培养，应用ＤＭＥＭ和ＤＭＥＭ＋１μｍｏｌ?Ｌ－１Ｄｅｘ作用于ＨＬＥＣ７ｄ，采用实时ＰＣＲ和Ｗｅｓｔｅｒｎ-ｂｌｏｔ从基因水平和蛋白水平检测波形蛋白的表达变化，采用免疫荧光染色法检测波形蛋白在细胞中的分布及表达变化。结果　实时ＰＣＲ结果显示，Ｄｅｘ组较对照组波形蛋白ｍＲＮＡ表达减少，组间差异有统计学意义（Ｐ＜０．０５）。Ｗｅｓｔｅｒｎ-ｂｌｏｔ检测结果显示，Ｄｅｘ组较对照组波形蛋白含量减少，组间差异有统计学意义（Ｐ＜０．０５），提示Ｄｅｘ可降低波形蛋白的表达。免疫荧光染色结果显示Ｄｅｘ组较对照组波形蛋白表达减少，与Ｗｅｓｔｅｒｎ-ｂｌｏｔ检测结果一致。结论　Ｄｅｘ可诱导ＨＬＥＣ中波形蛋白表达减少，从而引起ＨＬＥＣ异常的增殖和分化，最终导致糖皮质激素性白内障的形成。
Abstract:
 Objective To study the effects of dexamethasone ( Dex) on vimentin in human lens epithelial cells ( HLEC ) . and investigate the role of vimentin expression in steroid-induced cataract ( GIC ) formation. Methods HLEC were passaged and exposed to DMEM (control group) and DMEM + I ymol . L-l Dex ( Dex group) for 7 days. Real-time PCR and Western-blot were applied to identify the expression of vimentin at protein and transcription levels, the distribution and expression of vimentin in HLEC were measured by immumoflorescence stauung. Results Real-time PCR results showed that the expression of vimentin mRNA in Dex group was lower than that in control group (P < 0. 05 ) . Western-blot results showed that that the expression of vimentin protein in Dex group was lower than that in control group (P < 0. 05 ) .which suggested that Dex could reduce the expression of vimentin. Immunofluorescence stairung showed that the expression of vimentin in Dex group was lower than that in control group, which was consistent with the Western-blot results. Conclusion Dex can reduce the expression of vimentin in HLEC .which may cause abnormal proliferation and differentiation of HLEC , eventually leading to the formation of GIC.

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备注/Memo

备注/Memo:
 国家自然科学基金资助（编号：３０９７３２７５）；高等学校博士学科点专项科研基金资助（编号：２０１１２３０７１１００１３）
更新日期/Last Update: 2015-04-27