[1]马艳,吴志鸿,司南,等. αB-晶状体蛋白对急性高眼压大鼠视网膜神经节细胞保护作用机制的研究[J].眼科新进展,2015,35(4):318-322.[doi:10.13389/j.cnki.rao.2015.0085]
　MA Yan,WU Zhi-Hong,SI Nan,et al. Mechanism of αB-crystallin in protecting retinal ganglion cells of rats with acute ocular hypertension[J].Recent Advances in Ophthalmology,2015,35(4):318-322.[doi:10.13389/j.cnki.rao.2015.0085]
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 αB-晶状体蛋白对急性高眼压大鼠视网膜神经节细胞保护作用机制的研究
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年4期
页码:
318-322
栏目:
实验研究
出版日期:
2015-04-05

文章信息/Info

Title:
 Mechanism of αB-crystallin in protecting retinal ganglion cells of rats with acute ocular hypertension
作者:
 马艳; 吴志鸿; 司南; 周立冬; 韩毳; 王立华
 100039　北京市，武警总医院眼科
Author(s):
 MA Yan ;  WU Zhi-Hong;  SI Nan ;  ZHOU Li-Dong;  HAN Cui;  WANG Li-Hua
 Department of Ophthalmology, General Hospital of Chinese People ’ s Armed Police Forces, Beijing 100039 . China
关键词:
 ; αB-晶状体蛋白; 急性高眼压; 视网膜神经节细胞; 钾离子通道; Bcl-xl; Caspase-3
Keywords:
 ; αB-crystallin;  acute high intraocular pressure;  retinal ganglion cell;  potassium ion channels ;  Bcl-xl;  Caspase-3
DOI:
10.13389/j.cnki.rao.2015.0085
文献标志码:
A
摘要:
 目的　研究αＢ-晶状体蛋白对钾离子通道ｋｖ１．１、ｋｖ１．３及凋亡抑制因子Ｂｃｌ-ｘｌ和凋亡因子Ｃａｓｐａｓｅ-３的影响，探讨αＢ-晶状体蛋白对急性高眼压大鼠视网膜神经节细胞（ｒｅｔｉｎａｌｇａｎｇｌｉｏｎｃｅｌｌｓ，ＲＧＣ）保护作用的机制。方法　６０只ＳＤ大鼠随机分为Ａ组（急性高眼压组）、Ｂ组（急性高眼压＋玻璃体内注射生理盐水５μＬ组）、Ｃ组（急性高眼压＋玻璃体内注射１０×１０－６ｇ?Ｌ－１αＢ-晶状体蛋白５μＬ组），每组２０只，右眼为实验眼。注射后７ｄ和１４ｄ时，应用Ｗｅｓｔｅｒｎｂｌｏｔ和实时定量逆转录聚合酶链反应（ＱＲＴ-ＰＣＲ）法检测ｋｖ１．１、ｋｖ１．３、Ｂｃｌ-ｘｌ和Ｃａｓｐａｓｅ-３在视网膜上的表达水平。结果　注射后７ｄ和１４ｄ时，Ｃ组ｋｖ１．１、ｋｖ１．３通道蛋白和凋亡因子Ｃａｓｐａｓｅ-３蛋白表达水平和ｍＲＮＡ相对表达量均较Ａ组、Ｂ组低，差异均有统计学意义（均为Ｐ＜０.０５）；Ｃ组Ｂｃｌ-ｘｌ蛋白表达水平和ｍＲＮＡ相对表达量较Ａ组、Ｂ组高，差异均有统计学意义（均为Ｐ＜０．０５）；相同时间点不同因子Ａ组、Ｂ组间两两比较，差异均无统计学意义（均为Ｐ＞０．０５）。结论　αＢ-晶状体蛋白通过抑制急性高眼压后ＲＧＣ上钾离子通道ｋｖ１．１、ｋｖ１．３的表达，从而提高凋亡抑制因子Ｂｃｌ-ｘｌ的表达、降低凋亡因子Ｃａｓｐａｓｅ-３的表达，减少细胞凋亡，保护ＲＧＣ。
Abstract:
 Objective To investigate the mechanism of aB-crystallin in protecting retinal ganglion cells of rats with acute ocular hypertension through observing the effects of αB-crystallin on the expression of potassium ion channel kvl. I and kvl. 3. anti-apoptosis factors Bcl-xl and apoptotic factors Caspase-3. Methods Sixty SD rats were randomized into group A ( acute high intraocular pressure group) , group B ( acute high intraocular pressure following intravitreal injection of normal saline group) , group C ( acute high intraocular pressure following intravitreal injection of 10 x 10 -6 g . L -1 αB crystallin group) , 20 cases in each group , the right eye was the experimental eye. At 7 days and 14 days after injection, the expression of kvl. 1 . kvl. 3 . Bcl-xl and Caspase-3 in retina were observed by Western blot and QRT-PCR. Results At 7 days and 14 days after injection, the relative protein and mRNA expression levels of kvl. 1, kvl. 3 and Caspase-3 in group C were lower than those in group A and B, the difference was significant ( all P < 0. 05 ) ; The relative protein and mRNA expression levels of Bcl-xl in group C were higher than those in group A and B. the difference was significant ( all P < 0. 05 ) ; The relative protein and mRNA expression levels of BCL-XL in group C were higher than those in group A and B. the difference was significant ( all P < 0. 05 ) . There was no statistical difference in each index at each time point between group A and group B (P > 0. 05 ) . Conclusion αB-crystallin can inhibit the expression of potassium ion channels kvl. I . kvl. 3 in the retinal ganglion cells after acute high intraocular pressure , so it can increase the expression of anti-apoptosis factors Bcl-xl and decrease the expression of apoptotic factors Caspase-3 , then reduce apoptosis and protect RGC.

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备注/Memo

备注/Memo:
 中国博士后科学基金（编号：２００８０４３０４４４）
更新日期/Last Update: 2015-03-31