[1]林玲,任京力. 胆碱能神经元在闪光刺激增强大鼠初级视皮层可塑性中的作用[J].眼科新进展,2015,35(3):231-234.[doi:10.13389/j.cnki.rao.2015.0062]
　LIN Ling,REN Jing-Li. Effects of cholinergic neurons in flashing stimulation enhanced plasticity of primary visual cortex in rats[J].Recent Advances in Ophthalmology,2015,35(3):231-234.[doi:10.13389/j.cnki.rao.2015.0062]
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 胆碱能神经元在闪光刺激增强大鼠初级视皮层可塑性中的作用
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年3期
页码:
231-234
栏目:
实验研究
出版日期:
2015-03-05

文章信息/Info

Title:
 Effects of cholinergic neurons in flashing stimulation enhanced plasticity of primary visual cortex in rats
作者:
 林玲; 任京力
 462002　河南省漯河市，漯河医学高等专科学校生理教研室
Author(s):
 LIN Ling;  REN Jing-Li
 Department, Physiology of Luohe Medical College , Luohe 462002 , Henan Province , China
关键词:
 ; 初级视皮层; 视觉诱发电位; M受体; 胆碱能神经元; 胆碱乙酰转移酶; LTP
Keywords:
 ; primary visual cortex ;  visual evoked potential ;  M-receptor ;  cholinergic neurons ;  AchE ;  LTP
DOI:
10.13389/j.cnki.rao.2015.0062
文献标志码:
A
摘要:
 目的　研究胆碱能神经元及递质在大鼠初级视皮层可塑性中的作用。方法　３６只（３６眼）成年ＳＤ大鼠随机分为３组：对照组、激动剂组、阻断剂组，每组各１２只（１２眼）。各组大鼠麻醉、固定后在初级视皮层（Ｖ１区）植入记录电极和玻璃微电极、在额叶植入参考电极，术后第７、８、９天于固定时间行紫光闪光刺激１ｈ，第１０天行微量注射Ｍ受体激动剂卡巴胆碱和Ｍ受体阻断剂东莨菪碱，注射后在相同闪光刺激条件下给予一串高频刺激，记录各组高频刺激前后Ｖ１区的视觉诱发电位，分析各组视觉诱发电位的幅值和峰时值的变化。检测不同组别大鼠在强直性闪光刺激后初级视皮层中胆碱乙酰转移酶（ＣｈＡＴ）、胆碱酯酶（ＡｃｈＥ）活性，Ｗｅｓｔｅｒｎｂｌｏｔ检测不同组别大鼠初级视皮层ＣｈＡＴ含量。结果　对照组和激动剂组在接受强直性闪光刺激后视觉诱发电位幅值较之前有显著性升高（均为Ｐ＜０．０１），且峰时值变短（均为Ｐ＜０.０１），阻断剂组幅值和峰时值在刺激前后差异无统计学意义（均为Ｐ＞０．０５）。在强直性闪光刺激后，与对照组相比，激动剂组幅值升高（Ｐ＜０．０１）、峰时值变短（Ｐ＜０.０１），阻断剂组幅值降低（Ｐ＜０．０１）、峰时值变长（Ｐ＜０．０１）。对不同组别大鼠进行强直性闪光刺激后发现，与对照组相比，激动剂组的ＣｈＡＴ活性明显升高（Ｐ＜０．０５）、ＡｃｈＥ活性降低（Ｐ＜０．０５）；而阻断剂组的ＣｈＡＴ活性明显降低（Ｐ＜０．０５），ＡｃｈＥ活性升高（Ｐ＜０．０５）；阻断剂组与激动剂组ＣｈＡＴ、ＡｃｈＥ活性差异显著（均为Ｐ＜０．０５）。ＣｈＡＴ蛋白的表达经Ｗｅｓｔｅｒｎｂｌｏｔ分析发现，与对照组比较，激动剂组有显著性升高（Ｐ＜０．０５），阻断剂组有显著性降低（Ｐ＜０．０５），阻断剂与激动剂组差异显著（Ｐ＜０.０５）。结论　强直性闪光刺激能使大鼠闪光视觉诱发电位呈现ＬＴＰ样反应，胆碱能神经元及递质系统参与了闪光刺激致大鼠初级视皮层的可塑性变化。
Abstract:
 Objective To investigate the effects of cholinergic neurons and neurotransmitters in the plasticity of primary visual cortex in rats. Methods Thirty-six adult Sprague-Dawley rats were divided into three groups : control group , agorust group and blocker group, 12 cases ( 12 eyes) in each group. All of rats were anesthetized. fixed ,implanted recording electrode and glass microelectrode in the primary visual cortex ( V1 ) and reference electrode in the frontal lobe. Each group of rats were given violet flash stimulation at day 7 , day 8 , day 9 for I hour at fixed time after operation. M-receptor agorust and blocker were injected at day 10 , then a string of high-frequency flashing stimulation was applied to the primary visual cortex in the same flash stimulus condition. The flash visual evoked potential ( F-VEP) before and after high-frequency stimulation were recorded in the primary visual cortex ( V1 ) . and changes of amplitude , as well as peak latency were analyzed in each group. The activity of ChAT and AchE in primary visual cortex were checked after high frequency flash stimulation in different groups , and the expression of ChAT in different groups was detected by Western blot. Results After high-frequency stimulation.the amplitude of the F-VEP was sigruficantIy increased ( all P < 0. 01 ) , and the peak latency were significantly shorter ( all P < 0. 01 ) both in control group and agonist group. While in the blocker group , there was no significant difference ( all P > 0. 05 ) in amplitude and peak latency of the F-VEP. Mean-while , after high-frequency stimulation , compared with the control group , the amplitude of the F-VEP was significantly increased and the peak latency was significantly shorter ( all P < 0. 01) in the agonist group,while in the blocker group ,it showed amplitude reduction and peak latency lengthen of the F-VEP ( all P < 0. 01 ) . Compared with the control group after high frequency stimulation. the activity and expression of ChAT were both significantly increased in agonist group ( all P < 0. 05 ) .while decreased in blocker group ( all P < 0. 05 ) . The activity of AchE in agonist group was significantly reduced (P < 0. 05 ) and increased in blocker group ( P < 0. 05 ) compared with the control group. Conclusion High-frequency stimulation can make the LTP-Iike reaction in visual evoked potential. Cholinergic neurons and neurotransmitters contribute to the plasticity induced by flash stimulation in primary visual cortex.

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备注/Memo

备注/Memo:
 漯河医学高等专科学校２０１２校级自然科学研究资助项目（编号：２０１０Ｓ０７）
更新日期/Last Update: 2015-03-04