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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:

35卷

期数:

2015年3期

页码:

201-204

栏目:

实验研究

出版日期:

2015-03-05

文章信息/Info

Title:

 Role of macrophages in oxygen-induced retinal neovas-cularization in mice

作者:

 高翔; 王雨生

 710032　陕西省西安市，第四军医大学西京医院眼科

Author(s):

 GAO Xiang; WANG Yu-Sheng

 Department of Ophthalmology ,Xijing Hospital of the Fourth Military Medical University. Xi ’ an 710032 .Shaanxi Province , China

关键词:

 ; 巨噬细胞; 视网膜新生血管; 氧诱导视网膜病变

Keywords:

 ; macrophages ;  retinal neovascularization ;  oxygen-induced retinopathy

DOI:

10.13389/j.cnki.rao.2015.0055

文献标志码:

A

摘要:

 目的　探讨巨噬细胞对小鼠氧诱导视网膜病变（ｏｘｙｇｅｎ-ｉｎｄｕｃｅｄｒｅｔｉｎｏｐａｔｈｙ，ＯＩＲ）形成的影响。方法　将新生Ｃ５７ＢＬ／６Ｊ小鼠随机分为３组，分别为常氧对照组、ＯＩＲ模型组以及清除巨噬细胞的ＯＩＲ组。将后两组小鼠于生后第７天（Ｐ７）至Ｐ１２置于体积分数（７５±２）％高氧氧箱中以诱导ＯＩＲ。清除巨噬细胞的ＯＩＲ组小鼠于Ｐ９、Ｐ１１、Ｐ１３和Ｐ１５接受腹腔注射氯膦酸二钠脂质体４次以清除全身单核-巨噬细胞，ＯＩＲ模型组小鼠则于上述４个时间点接受腹腔注射ＰＢＳ脂质体。三组小鼠均于Ｐ１７时取右眼行视网膜铺片和Ｌｅｃｔｉｎ染色，观察视网膜出血及血管生长情况；取左眼行视网膜切片和ＨＥ染色，观察视网膜组织病理学变化。结果　与常氧对照组相比，ＯＩＲ模型组小鼠视网膜存在出血现象，清除巨噬细胞的ＯＩＲ组出血有所减轻。ＯＩＲ模型组小鼠视网膜血管形态呈异常增殖的团簇状，走行迂曲，而清除巨噬细胞的ＯＩＲ组小鼠视网膜新生血管异常形态减轻。与ＯＩＲ模型组相比，清除巨噬细胞的ＯＩＲ小鼠视网膜无血管区及新生血管区面积均显著减小［（１７．１９±０．５８）％、（１０．３８±０．５３）％，ｔａ＝８．６８０，Ｐ＜０．０１；（４．６０±０．１５）％、（２．５１±０．１３）％，ｔｎ＝１０．８３，Ｐ＜０．０１］，突破内界膜新生血管内皮细胞核数目明显减少（１８．５０±０．８５、７．１７±０．４８；ｔ＝１１．６６，Ｐ＜０．０１）。结论　清除巨噬细胞可减轻小鼠ＯＩＲ严重程度，提示巨噬细胞对视网膜新生血管形成具有促进作用。

Abstract:

 Objective To investigate the role of macrophages in the formation of oxygen-induced retinopathy ( OIR) in mice. Methods Neonatal C57BL/6J nuce were divided randomly into three groups : normoxic control group , OIR control group and OIR group with macrophages depleted. The mice of the latter two groups were exposed to 75 % oxygen from P7 to P12 to induce OIR. The macrophages were depleted by injection of clodronate-liposomes ( lip) intraperitoneally at P9 ,PII ,P13 and P15. The mice of OIR control group were treated with PBS-Iip at the same time. All eyes were collected at P17. The right eyes were wholemounted and stained with Lectin to observe the retinal hemorrhage and the growth of retinal vessels ; The left eyes were performed histopathological cross-sections stained with H&E to analyze the severity of retinal neovascularization. Results The OIR control mice exhibited retinal hemorrhage compared with normoxic control group, and the hemorrhage reduced by the macrophage depletion. The retinal vessels in OIR control group were tortuous with abnormally proliferative cluster structures. The abnormal structures of oxygen-induced retinal neovascularization were improved after the macrophage depleted. The retinal avascular area. neovascular area were markedly decreased after the macrophage depletion compared with OIR control group [ ( 17. 19 +0. 58 ) % vs. ( 10. 38 +0. 53) % ; to = 8. 680 ,P < 0. 01 ; (4. 60 +0. 15 ) % vs. (2. 51 + 0. 13 ) % ; tn = 10. 83 ,P < 0. 01 ] . The number of vascular cell nuclei anterior to the retinal internal limiting membrane were notably reduced after the macrophage depleted compared with OIR control group ( 18. 50 + 0. 85 vs. 7. 17 +0. 48 ; t = 11. 66 ,P < 0. 01). Conclrision The oxygen-induced retinopathy is alleviated after the macrophage depleted , which indicate that macrophages may play a proangiogenic role in retinal neovascularization.

备注/Memo

备注/Memo:

 国家自然科学基金资助（编号：８１２７１０１４、８１４７０６５５）

常用功能

更新日期/Last Update: 2015-03-04