[1]李云琴,邹悦,胡竹林,等. 人脐血干细胞玻璃体腔移植对大鼠外伤性视神经损伤的保护作用[J].眼科新进展,2015,35(2):125-128.[doi:10.13389/j.cnki.rao.2015.0033]
　LI Yun-Qin,ZOU Yue,HU Zhu-Lin,et al. Protective effects of human umbilical cord blood stem cells vitreous transplantation on function restoration after traumatic optic nerve injury[J].Recent Advances in Ophthalmology,2015,35(2):125-128.[doi:10.13389/j.cnki.rao.2015.0033]
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 人脐血干细胞玻璃体腔移植对大鼠外伤性视神经损伤的保护作用
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年2期
页码:
125-128
栏目:
实验研究
出版日期:
2015-02-05

文章信息/Info

Title:
 Protective effects of human umbilical cord blood stem cells vitreous transplantation on function restoration after traumatic optic nerve injury
作者:
 李云琴; 邹悦; 胡竹林; 肖丽波; 吴敏; 刘梅; 陈怡颖; 张颖
 650021　云南省昆明市，云南省第二人民医院眼科
Author(s):
 LI Yun-Qin;  ZOU Yue;  HU Zhu-Lin;  XIAO Li-Bo;  WU Min;  LIU Mei;  CHEN Yi-Ying ;  ZHANG Ying
 Department of Ophthalmology,the Second People ’ s Hospital of Yunnan Province , Kunming 650021 , Yunnan Province . China
关键词:
 ; 人脐血干细胞; 视神经损伤; 移植; 视网膜神经节细胞
Keywords:
 ; human umbilical cord blood stem cell;  optic nerve injury ;  transplantation ;  retinal ganglion cell
DOI:
10.13389/j.cnki.rao.2015.0033
文献标志码:
A
摘要:
 目的　观察人脐血干细胞（ｈｕｍａｎｕｍｂｉｌｉｃａｌｃｏｒｄｂｌｏｏｄｓｔｅｍｃｅｌｌｓ，ｈＵＣＢＳＣ）移植对视神经部分受损ＳＤ大鼠视网膜神经节细胞（ｒｅｔｉｎａｌｇａｎｇｌｉｏｎｃｅｌｌｓ，ＲＧＣ）的保护作用。方法　将４８只健康成年ＳＤ大鼠随机分为２组，２组ＳＤ大鼠暴露视神经，应用４０ｇ夹持力的视神经夹在大鼠眼球后２ｍｍ处夹视神经３０ｓ造成部分视神经损伤模型，均损伤左眼。Ａ组：伤后１周玻璃体腔注射脐血干细胞载体ＰＢＳ，２４只。Ｂ组：伤后１周玻璃体腔注射ｈＵＣＢＳＣ，２４只；２组均于注射后７ｄ、１４ｄ、２１ｄ、２８ｄ处死动物。处死前７ｄ双上丘注射５０ｇ?Ｌ－１荧光金逆行标记双眼ＲＧＣ。然后按时间先后处死大鼠分离视网膜置于荧光显微镜下，计数２组ＲＧＣ并计算ＲＧＣ标识率。结果　Ａ、Ｂ两组视神经损伤眼ＲＧＣ计数均低于未损伤眼（均为Ｐ＜０．０５），且随时间延长两组ＲＧＣ标识率均呈下降趋势（均为Ｐ＜０．０５），但Ｂ组注射后７ｄ、１４ｄ、２１ｄ、２８ｄＲＧＣ标识率（７７．５２±６．３３）％、（７４．１２±８.２３）％、（６４．７８±５.２１）％、（５９．９３±９．００）％下降幅度明显较Ａ组（７５．６８±８．７４）％、（６８．２１±１０．５９）％、（５６．２４±９．３４）％、（４８．９１±８．８１）％平缓。同时间段Ｂ组ＲＧＣ标识率均高于Ａ组，且差异均有统计学意义（均为Ｐ＜０．０５）。结论　玻璃体腔注射ｈＵＣＢＳＣ可减缓外伤性视神经损伤大鼠视网膜中ＲＧＣ的凋亡，对ＲＧＣ具有一定的保护作用。
Abstract:
 Objective To observe the protective effects of human umbilical cord blood stem cells ( hUCBSC) vitreous transplantation on retinal ganglion cells ( RGC) in the Spragur-Dawly rats with optic nerve injury. Methods Forty-eight adult Sprague-Dawley rats were randomly divided into two groups, 24 rats in each group. Calibrated optic nerve crush injury model was induced in the left eyes in the six groups , the right eyes served as control. Medicine was injected at 7 days after optic nerve injury. PBS was injected into vitreous of group A. The hUCBSC were injected into vitreous of group B. And then the rats were sacrificed at 7 days,14 days ,21 days and 28 days. Seven days before sacrifice ,50 g . L -l fluorogold was injected into superior colliculi bilaterally to label the RGC. The eyes were enucleated. and flat mounts of the retina from both eyes were prepared on a slide and observed under a fluorescence microscope. The labeled RGC were counted by a computerized image analyzer, and the labeled RGC rate was used for statistical analysis. Results The number of RGC in the injury eye was less than that in the control eye in group A , B ( all P < 0. 05 ) . The labeled RGC rate showed a declined tendency gradually along with increases of the time in two groups ( all P < 0. 05 ) , but the decreased trend of group B, which were ( 77. 52 + 6. 33 ) % at 7 days, ( 74. 12 + 8. 23 ) % at 14 days , ( 64. 78 +5. 21) % at 21 days and (59. 93 +9. 00) % at 28 days ,were evidently slower than those of group A, which were ( 75. 68 + 8. 74) % at 7 days , ( 68. 21 + 10. 59) % at 21 days , ( 56. 24 + 9. 34) % at 24 days and (48. 91 +8. 81) % at 28 days , there were statistical differences between two groups ( all P < 0. 05 ) . Conclusion The vitreous injection of hUCBSC can decrease the apoptosis of RGC in ratina of rats with traumatic optic nerve injury , and has the protective effect on RGC.

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备注/Memo

备注/Memo:
 云南省科技厅资助项目（编号：２０１２ＦＢ２０６、２０１２ＦＢ０８３）
更新日期/Last Update: 2015-02-03