[1]杨建刚,何娜,孙鹏锐,等. p27Kip1高表达对兔眼滤过术后成纤维细胞增殖作用的影响[J].眼科新进展,2015,35(2):120-124.[doi:10.13389/j.cnki.rao.2015.0032]
　YANG Jian-Gang,HE Na,SUN Peng-Rui,et al. Effect of p27Kipl overexpression on fibroblasts proliferation in rabbit after filtration surgery[J].Recent Advances in Ophthalmology,2015,35(2):120-124.[doi:10.13389/j.cnki.rao.2015.0032]
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 p27Kip1高表达对兔眼滤过术后成纤维细胞增殖作用的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年2期
页码:
120-124
栏目:
实验研究
出版日期:
2015-02-05

文章信息/Info

Title:
 Effect of p27Kipl overexpression on fibroblasts proliferation in rabbit after filtration surgery
作者:
 杨建刚; 何娜; 孙鹏锐; 王小娣; 姚囯敏; 刘倩影
 710002　陕西省西安市，西安市第一医院眼科
Author(s):
 YANG Jian-Gang;  HE Na;  SUN Peng-Rui;  WANG Xiao-Di;  YAO Guo-Min ;  LIU Qian-Ying
 Department of Ophthalmology,the First Hospital of Xi’an, Xi’an 710002, Shaanxi Province,China
关键词:
 ; 细胞周期依赖性激酶抑制剂; p27Kip1; 青光眼; 滤过术; 细胞增殖; 兔
Keywords:
 ; cyclin dependent kinase inhibitor;  p27Kip1 ;  glaucoma;  filtration surgery ;  proliferation ;  rabbit
DOI:
10.13389/j.cnki.rao.2015.0032
文献标志码:
A
摘要:
 目的　研究外源性ｐ２７Ｋｉｐ１对兔眼滤过术后成纤维细胞ｐ２７Ｋｉｐ１、ＰＣＮＡ和ＡｇＮＯＲ表达的影响。方法　构建腺病毒为载体的ｐ２７Ｋｉｐ１（Ａｄ-ｐ２７），长耳白兔２４只随机分为Ａｄ-ｐ２７组和对照组，行单眼滤过术，术中结膜下分别注射Ａｄ-ｐ２７和磷酸盐缓冲液，术后７ｄ、１４ｄ、２１ｄ和２８ｄ免疫组织化学染色法评价手术区成纤维细胞ｐ２７Ｋｉｐ１和ＰＣＮＡ的表达，ＡｇＮＯＲ染色观察成纤维细胞核仁的ＡｇＮＯＲ颗粒计数。结果　Ａｄ-ｐ２７作用后ｐ２７Ｋｉｐ１在Ｔｅｎｏｎ囊成纤维细胞主要为核表达，术后７ｄ表达即增高，阳性细胞为７９．１７％，细胞评分为（６．４０±０．６７）分，术后２８ｄ时阳性细胞为２７．０３％，评分为（４．９７±０．８３）分；对照组术后７ｄ和２８ｄ时细胞评分分别为（４．８８±０．７９）分和（２．４５±０．６７）分，两组相比差异均具有统计学意义。Ａｄ-ｐ２７组ＰＣＮＡ的表达降低，术后１４ｄ始细胞评分与对照组相比差异具有统计学意义，持续至术后２８ｄ。术后２８ｄ内Ａｄ-ｐ２７组ＡｇＮＯＲ染色始终为低表达，术后７ｄ颗粒计数为（２．１８±０．７２）个，较对照组的（４．０４±０．６８）个显著降低（Ｐ＝０．０００），２８ｄ时颗粒计数为（２．１３±０．６９）个，较对照组的（４.０６±０.８５）个显著降低（Ｐ＝０.０００）。结论　ｐ２７Ｋｉｐｌ高表达可以抑制兔眼滤过术后Ｔｅｎｏｎ囊成纤维细胞的增殖，减少术后瘢痕形成，提高手术成功率。
Abstract:
 Objective To investigate the effects of exogenous p27Kipl on p27Kipl , PCNA and AgNOR expression in Tenon ’ s fibroblasts on rabbit eyes after filtration surgery. Methods The p27Kip1-expressing adenovirus ( Ad-p27) was constructed. Twenty-four rabbits were divided randomly into Ad-p27 group and control group. The unilateral eyes were performed filtration surgery. Ad-p27 0r PBS was injected into subconjunctiva during surgery ,respectively. The expressions of p27 Kipl or PCNA in fibroblasts were analyzed at surgical sites at 7 days ,14 days ,21 days and 28 days after surgery by immunohistochemistry. The numbers of nucleolar AgNOR particles were counted in fibroblasts by AgNOR staining. Results p27 Kipl expression was mainly observed in the nucleus of fibroblasts induced by Ad-p27. The expression was increased significantly at 7 days. The positive cells were 79. 17% in Ad-p27 group , the cell scores were 6. 40 + 0. 67 ; The expression remained high level at 28 days, the positive cells were 27. 03% . and the cell scores were 4. 97 +0. 83 ; The cell scores in control group was 4. 88 + 0. 79 and 2. 45 + 0. 67 ,there were statistical differences between two groups. The expression of PCNA was decreased significantly in Ad-p27 group, there was statistical difference in cell scores at 14 days compared with control group. The expressions of AgNOR were persistently decreased within 28 days in Ad-p27 group, the number of AgNOR particles at 7 days and 28 days were 2. 18 + 0. 72 and 2. 13 + 0. 69 . respectively, compared with the control group (4. 04 + 0. 68 and 4. 06 + 0. 85 ) . there were significant differences ( both P = 0. 000) . Conclrision The overexpression of p27K’pl can inhibit the proliferation of Tenon fibroblasts after filtration surgery , inhibit the scar formation and promote the surgical outcome.

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备注/Memo

备注/Memo:
 陕西省教育厅科研计划项目（编号：１１ＪＫ７０５）；西安市科技计划项目［编号：ＨＭ１１１６（５）］
更新日期/Last Update: 2015-02-03