[1]底煜,张轶欧,王爱媛,等.Cyr61和VEGF在高氧诱导鼠视网膜新生血管中的表达[J].眼科新进展,2015,35(1):015-17.[doi:10.13389/j.cnki.rao.2015.0004]
　DI Yu,ZHANG Yi-Ou,WANG Ai-Yuan,et al.Expression of Cyr61 and VEGF in hyperxia-induced retinal neovascularization of mice[J].Recent Advances in Ophthalmology,2015,35(1):015-17.[doi:10.13389/j.cnki.rao.2015.0004]
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Cyr61和VEGF在高氧诱导鼠视网膜新生血管中的表达
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年1期
页码:
015-17
栏目:
实验研究
出版日期:
2015-01-05

文章信息/Info

Title:
Expression of Cyr61 and VEGF in hyperxia-induced retinal neovascularization of mice
作者:
底煜; 张轶欧; 王爱媛; 陈晓隆
１１０００４　辽宁省沈阳市，中国医科大学附属盛京医院眼科
Author(s):
DI Yu ;  ZHANG Yi-Ou ;  WANG Ai-Yuan;  CHEN Xiao-Long
Department of Ophthalmology , Shengjing Hospital of China Medical University , Shenyang 110004 . Liaoning Province , China
关键词:
视网膜新生血管; 富含半胱氨酸蛋白61; 血管内皮生长因子
Keywords:
retinal neovascularization;  cysteine-rich 61 ;  vascular endothelial growth factor
DOI:
10.13389/j.cnki.rao.2015.0004
文献标志码:
A
摘要:
目的　研究富含半胱氨酸蛋白６１（ｃｙｓｔｅｉｎｅｒｉｃｈ６１，Ｃｙｒ６１）和血管内皮生长因子（ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＶＥＧＦ）在高氧诱导鼠视网膜新生血管（ｒｅｔｉｎａｌｎｅｏｖａｓｃｕｌａｒｉｚａｔｉｏｎ，ＲＮＶ）中的表达情况，探讨二者在ＲＮＶ形成过程中所起的作用。方法　取Ｃ５７ＢＬ／６Ｊ小鼠１００只，随机分为高氧组和对照组，每组各５０只。采用免疫组织化学、Ｗｅｓｔｅｒｎｂｌｏｔ和ＲＴＰＣＲ法分别检测视网膜Ｃｙｒ６１、ＶＥＧＦ蛋白及ｍＲＮＡ的表达情况。结果　高氧组Ｃｙｒ６１、ＶＥＧＦ蛋白呈强阳性表达，主要定位于神经节细胞层、内丛状层、外丛状层和突破视网膜内界膜的新生血管。高氧组与对照组比较，视网膜Ｃｙｒ６１、ＶＥＧＦ蛋白及ｍＲＮＡ表达显著增高，均有统计学意义（ｔＣｙｒ６１蛋白＝５．３５、ｔＶＥＧＦ蛋白＝６．５６、ｔＣｙｒ６１ｍＲＮＡ ＝４．６７、ｔＶＥＧＦｍＲＮＡ ＝７．１５，均为 Ｐ＜０．０５），且Ｃｙｒ６１与ＶＥＧＦ表达呈正相关（ｒ蛋白＝０．８３、ｒｍＲＮＡ ＝０９５，均为Ｐ＜０．０５）。结论　Ｃｙｒ６１ＶＥＧＦ信号途径在视网膜新生血管形成的过程中可能具有潜在功能。
Abstract:
Objective To explore the expression and significance of cysteinerich 61 ( Cyr61) and vascular endothelial growth factor ( VEGF ) in hyperxia-induced retinal neovascularization of mice. Methods One hundred healthy C57BL/6J mice were chosen and randomly divided into control group and hyperoxic group , 50 mice in each group. Protein and mRNA expression of Cyr61 and VEGF were detected by immunohistochemical . Western blot and RT-PCR. Results Cyr61 and VEGF protein expressions were strongly labeled in the ganglion cell layer ,inner plexiform layer, outer plexiform layer and neovascularization breaking through the intemal limiting membrane in the hyperoxic group. Compared with the control group ,there were high protein and mRNA expression of Cyr61 and VEGF in the hyperoxic group ,the differences were statistical sigruficant( tC.yffil protem = 5. 35 , tVEGF protein = 6. 56 , tCyr61 mRNA = 4. 67 , tVEGF mRNA = 7. 15 , all P < 0. 05 ) , and there was an obvious positive correlation between the expression of Cyr61 and VEGF( rprotein = 0. 83 , rmRNA = 0. 95 , all P < 0. 05 ) . Conclusion Cyr61-VEGF signaling pathway may have potential function in the retinal neovascularization.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：８１３７１０４５）；辽宁省科技计划项目基金资助（编号：２０１０２２５０３４）
更新日期/Last Update: 2015-01-04