[1]赵永旺,符超君,颜家朝,等.双丹明目胶囊对糖尿病视网膜病变大鼠血液流变学和视网膜微血管的影响[J].眼科新进展,2022,42(5):342-346.[doi:10.13389/j.cnki.rao.2022.0069]
　ZHAO Yongwang,FU Chaojun,YAN Jiachao,et al.Effects of Shuangdan Mingmu capsules on blood rheology and retinal microvasculature in rats with diabetic retinopathy[J].Recent Advances in Ophthalmology,2022,42(5):342-346.[doi:10.13389/j.cnki.rao.2022.0069]
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双丹明目胶囊对糖尿病视网膜病变大鼠血液流变学和视网膜微血管的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
42卷
期数:
2022年5期
页码:
342-346
栏目:
实验研究
出版日期:
2022-05-05

文章信息/Info

Title:
Effects of Shuangdan Mingmu capsules on blood rheology and retinal microvasculature in rats with diabetic retinopathy
作者:
赵永旺; 符超君; 颜家朝; 聂辅娇; 陈佳玉; 郑新宝; 杨雪艳; 秦裕辉
410208　湖南省长沙市，湖南中医药大学（赵永旺，符超君，颜家朝，聂辅娇，秦裕辉）；201600　上海市，上海市松江区中心医院（赵永旺，陈佳玉，郑新宝）；425000　湖南省永州市，永州市第一人民医院（杨雪艳）
Author(s):
ZHAO Yongwang1; 2; FU Chaojun1; YAN Jiachao1; NIE Fujiao1; CHEN Jiayu2; ZHENG Xinbao2; YANG Xueyan3; QIN Yuhui1
1.Hunan University of Traditional Chinese Medicine,Changsha 410208， Hunan Province,China
2.Songjiang District Central Hospital,Shanghai 201600,China
3.Yongzhou First People’s Hospital,Yongzhou 425000，Hunan Province,China
关键词:
双丹明目胶囊; 糖尿病视网膜病变; 血液流变学; 微血管
Keywords:
Shuangdan Mingmu capsules;  diabetic retinopathy;  blood rheology;  microvasculature
分类号:
R774
DOI:
10.13389/j.cnki.rao.2022.0069
文献标志码:
A
摘要:
目的　探讨双丹明目胶囊对糖尿病视网膜病变（DR）大鼠血液流变学和视网膜微血管的影响。方法　取SPF级雄性SD大鼠72只，将大鼠随机分为6组：正常组、模型组、对照组、高剂量组、中剂量组和低剂量组，每组12只。正常组和模型组大鼠给予生理盐水，对照组大鼠给予羟苯磺酸钙（等效给药浓度为5.8 mg·kg-1），高、中、低剂量组大鼠分别给予不同剂量的双丹明目胶囊（22.4 g·kg-1、11.2 g·kg-1、5.6 g·kg-1），分别是临床等效剂量的2.0倍、1.0倍、0.5倍，各组均按10 mL·kg-1剂量灌胃。每周观察大鼠一次，连续12周，检测或量化相关指标。血液流变学指标包括全血黏度（WBV）、血浆黏度（PV）、红细胞沉降率（ESR）、红细胞压积（HCT）、纤维蛋白原含量（FIB）。对各个时期SD大鼠眼底图片通过海德堡共聚焦激光造影仪自带软件进行分析，对大鼠眼底视网膜动脉、静脉血管管径和视网膜微血管荧光素渗漏面积进行量化。结果　连续灌胃12周后，与模型组相比，对照组和高剂量组大鼠WBV、PV、ESR、HCT、FIB均降低（均为P<0.05），中剂量组HCT也降低（P<0.01）；与高剂量组相比，低剂量组大鼠WBV、PV、ESR、HCT、FIB均升高，中剂量组大鼠除HCT外，各项指标亦均升高（均为P<0.01）。造模成功后，与正常组比较，其他各组大鼠视网膜动脉血管管径均变细，静脉血管管径均增粗（均为P<0.05）。干预12周后，高剂量组大鼠视网膜动脉血管管径接近正常组，静脉血管管径仍然变粗（均为P<0.05）；其他各组大鼠视网膜动脉血管管径普遍变细，静脉血管管径迂曲扩张（均为P<0.05）。与模型组相比，对照组和高剂量组大鼠视网膜微血管荧光素渗漏面积减小（均为P<0.01）；与对照组和高剂量组相比，中剂量组、低剂量组大鼠视网膜微血管荧光素渗漏面积均增大（均为P<0.01）。结论　双丹明目胶囊可以改善DR大鼠血液流变学状态，扩张视网膜动脉，改善视网膜血供，减少视网膜微血管荧光素渗漏面积。
Abstract:
Objective　To investigate the effects of Shuangdan Mingmu capsules on blood rheology and retinal microvasculature in rats with diabetic retinopathy (DR). Methods　Totally 72 SPF-grade male SD rats were randomly divided into six groups: normal group, model group, control group, high-dose group, medium-dose group, and low-dose group, with 12 rats in each group. The rats in the normal and model groups were given normal saline, the rats in the control group were given calcium dobesilate (equivalent drug concentration of 5.8 mg·kg-1), and the rats in the high-, medium- and low-dose groups were given different doses of Shuangdan Mingmu capsules (22.4 g·kg-1, 11.2 g·kg-1 and 5.6 g·kg-1), which were 2.0, 1.0 and 0.5 times of the clinical equivalent doses, respectively. All rats were fed with 10 mL·kg-1 volume by gavage. The rats were observed once a week for 12 weeks, and the relevant indexes were measured or quantified. Hemorheological indexes included whole blood viscosity (WBV), plasma viscosity (PV), erythrocyte sedimentation rate (ESR), hematocrit (HCT), and fibrinogen content (FIB). Fundus pictures of SD rats in each period were analyzed by Heidelberg Confocal Laser Angiograph self-contained software to quantify the fundus retinal arterial and venous vessel diameters and retinal microvascular fluorescein leakage area. Results　After 12 weeks of continuous gavage, WBV, PV, ESR, HCT, and FIB were reduced in the control and high-dose groups compared with the model group (all P<0.05), and HCT was also reduced in the medium-dose group (P<0.01); compared with the high-dose group, WBV, PV, ESR, HCT, and FIB were increased in the low-dose group, and all indexes were increased in the medium-dose group except for HCT (all P<0.01). After successful modeling, compared with the normal group, the retinal arterial vascular diameters in other groups were thinner, and the venous vascular diameters were thicker (all P<0.05). After 12 weeks of intervention, the retinal arterial vascular diameters of rats in the high-dose group were close to those of rats in the normal group, while the venous vascular diameters were still thickened (all P<0.05); the retinal arterial vascular diameters of rats in the rest groups were thinner, and the venous vascular diameters were thicker (all P<0.05). Compared with the model group, the area of retinal microvascular fluorescein leakage in the control and high-dose groups decreased (all P<0.01); compared with the control and high-dose groups, the area of retinal microvascular fluorescein leakage in the medium- and low-dose groups increased (all P<0.01). Conclusion　Shuangdan Mingmu capsules can improve the blood rheological status of DR rats, dilate retinal arteries, improve retinal blood supply, and reduce the area of retinal microvascular fluorescein leakage.

参考文献/References:

［1］　彭清华.中医眼科学［M］.第4版.北京：中国中医药出版社，2016：175.
PENG Q H.Ophthalmology of traditional Chinese medicine［M］.4th ed.Beijing:China Traditional Chinese Medicine Press,2016:175.
［2］　CAI X,MCGINNIS J F.Diabetic retinopathy:animal models,therapies,and perspectives［J］.J Diabetes Res,2016,2016:3789217.
［3］　HAMMES H P,FENG Y,PFISTER F,BROWNLEE M.Diabetic retinopathy:targeting vasoregression［J］.Diabetes,2011,60(1):9-16.
［4］　QIN Y H,LI W J,ZHANG X,DAI Z S,CHEN X L,ZHOU Y S,et al.Effects of Shuangdan Mingmu capsules on retinal vascular morphology and VEGF expression in ratswith diabetic retinopathy［J］.Int Eye Sci,2014,14(11):1943-1945.
［5］　符超君,凌艳君,颜家朝,赵永旺,刘峥嵘,秦裕辉.双丹明目胶囊对糖尿病视网膜病变大鼠血糖血脂及血液流变学的影响［J］.湖南中医药大学学报,2018,38(5):489-492.
FU C J,LING Y J,YAN J C,ZHAO Y W,LIU Z R,QIN Y H,et al.Effects of Shuangdan Mingmu capsules on blood glucose,blood lipid and hemorheology in diabetic retinopathy rats［J］.J Hunan Univ Tradit Chin Med,2018,38(5):489-492.
［6］　秦裕辉,李芳,涂良钰,邱波,张明亮,曹建辉,等.双丹明目胶囊治疗糖尿病视网膜病变的多中心临床研究［J］.湖南中医药大学学报,2010,30(1):46-51.
QIN Y H,LI F,TU L Y,QIU B,ZHANG M L,CAO J H,et al.Multicentric clinical study of Shuangdan Mingmu capsules on diabetic retinopathy［J］.J Hunan Univ Tradit Chin Med,2010,30(1):46-51.
［7］　TING D S,CHEUNG G C,WONG T Y.Diabetic retinopathy:global prevalence,major risk factors,screening practices and public health challenges:a review［J］.Clin Exp Ophthalmol,2016,44(4):260-277.
［8］　WU J,WANG R,YE Z,SUN X,CHEN Z,XIA F,et al.Protective effects of methane-rich saline on diabetic retinopathy via anti-inflammation in a streptozotocin-induced diabetic rat model［J］.Biochem Biophys Res Commun,2015,466(2):155-162.
［9］　HENRY F,PAQUET P,PIRARD-FRANCHIMONT C,PIRARD G E.Leg heaviness and stasis microangiopathy.Preventive and therapeutic approaches［J］.Rev Med Liege,2003,58(6):435-438.
［10］　何剑峰,杜娟,唐柳萍,李向荣,黄艳霞.糖尿病大鼠视网膜微血管变化与血液流变学的关系［J］.中国临床康复,2005(31):93-95,257.
HE J H,DU J,TANG L P,LI X R,HUANG Y X.Relationship between retinal microvascular changes and hemorheology in diabetic rats ［J］.Chin J Clin Rehabilit,2005,31 (9):93-95,257.
［11］　KIRBAS C,QUEK F.A review of vessel extraction techniques and algorithms［J］.ACM Comput Surv,2004,36(2):81-121.
［12］　CHAARI A,KSSIBI H,ZRIBI W,MEDHIOUB F,CHELLY H,ALGIA N B,et al.Ventilator-associated pneumonia in trauma patients with open tracheotomy:predictive factors and prognosis impact［J］.J Emerg Trauma Shock,2013,6(4):246-251.
［13］　CHRISTOPHE B R,MEHTA S H,GARTON A L,SISTI J,CONNOLLY E J.Current and future perspectives on the treatment of cerebral ischemia［J］.Expert Opin Pharmacother,2017,18(6):573-580.
［14］　隋亚楠.复方血栓通胶囊联合羟苯磺酸钙治疗糖尿病视网膜病变的疗效观察［J］.内蒙古中医药,2017,36(8):93.
SUI Y N.Efficacy observation of compound Xueshuantong capsule combined with calcium dobesilate in the treatment of diabetic retinopathy ［J］.Nei Mongol J Tradit Chin Med,2017,36(8):93.
［15］　肖立萍.复方血栓通联合羟苯磺酸钙治疗非增殖期糖尿病视网膜病变的临床疗效观察［J］.吉林医学,2016,37(9):2254-2255.
XIAO L P.Clinical observation of compound Xueshuantong combined with calcium dobesilate in the treatment of non proliferative diabetic retinopathy ［J］.Jilin Med,2016,37(9):2254-2255.
［16］　MIURA M,HONG Y J,YASUNO Y,MURAMATSU D,IWASAKI T,GOTO H.Three-dimensional vascular imaging of proliferative diabetic retinopathy by Doppler optical coherence tomography［J］.Am J Ophthalmol,2015,159(3):528-538.
［17］　SILVA P S,CAVALLERANO J D,TOLLS D,OMAR A,THAKORE K,PATEL B,et al.Potential efficiency benefits of nonmydriatic ultrawide field retinal imaging in an ocular telehealth diabetic retinopathy program［J］.Diabetes Care,2014,37(1):50-55.
［18］　周伟,靳春杰,孟兆联,吴建国,颜华.糖尿病视网膜病变模型鼠品系的选择［J］.中华实验眼科杂志,2014,32(1):32-35.
ZHOU W,JIN C J,MENG Z L,WU J G,YAN H.Selection of diabetic retinopathy model mouse strains［J］.Chin J Exp Ophthalmol,2014,32(1):32-35.
［19］　龙红萍,秦裕辉,刘峥嵘,王宇红,喻嵘.UPLC-Q-TOF 法分析双丹明目胶囊化学成分［J］.中成药,2017,32(7):1527-1531.
LONG H P,QIN Y H,LIU Z R,WANG Y H,YU R.Analysis of chemical constituents of Shuangdan Mingmu capsule by UPLC-Q-TOF［J］.Chin Tradit Patent Med,2017,32(7):1527-1531.
［20］　RIBEIRO M L,SERES A I,CARNEIRO A M,STUR M,ZOURDANI A,CAILLON P,et al.Effect of calcium dobesilate on progression of early diabetic retinopathy:a randomised double-blind study［J］.Graefes Arch Clin Exp Ophthalmol,2006,244(12):1591-1600.
［21］　VELPANDIAN T,NIRMAL J,GUPTA P,VIJAYAKUMAR A R,GHOSE S.Evaluation of calcium dobesilate for its anti-cataract potential in experimental animal models［J］.Methods Find Exp Clin Pharmacol,2010,32(3):171-179.
［22］　郑广瑛,蒋瑜,王瑞娜,郝莉莉,徐东.羟苯磺酸钙对大鼠半乳糖白内障抑制作用的研究［J］.中华眼科杂志,2013,49(10):927-933.
ZHENG G Y,JIANG Y,WANG R N,HAO L L,XU D.Study on the inhibitory effect of Calcium dobesilate on galactose cataract in rats［J］.Chin J Ophthalmol,2013,49(10):927-933.

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　ZHENG Xinbao,CHEN Jiayu,YANG Aiping,et al.Protective effect of Shuangdan Mingmu Capsules on the retina of rats with diabetic retinopathy and its mechanism[J].Recent Advances in Ophthalmology,2023,43(5):279.[doi:10.13389/j.cnki.rao.2023.0057]

备注/Memo

备注/Memo:
国家自然科学基金资助（编号：81874493）；湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金资助项目（编号：03030301301004）；湖南省研究生科研创新项目（编号：2018CX10，CX201910581）
更新日期/Last Update: 2022-05-06