[1]时倩倩,高彦,王李理,等.杞菊地黄汤对糖尿病视网膜病变模型大鼠视网膜新生血管的作用[J].眼科新进展,2020,40(4):327-331.[doi:10.13389/j.cnki.rao.2020.0075]
　SHI Qianqian,GAO Yan,WANG Lili,et al.Effect of Qiju Dihuang Decoction on retinal angiogenesis in diabetic retinopathy model rats[J].Recent Advances in Ophthalmology,2020,40(4):327-331.[doi:10.13389/j.cnki.rao.2020.0075]
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杞菊地黄汤对糖尿病视网膜病变模型大鼠视网膜新生血管的作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
40卷
期数:
2020年4期
页码:
327-331
栏目:
实验研究
出版日期:
2020-04-05

文章信息/Info

Title:
Effect of Qiju Dihuang Decoction on retinal angiogenesis in diabetic retinopathy model rats
作者:
时倩倩; 高彦; 王李理; 孙广莉; 陈秀英; 李晓鹏; 崔龙江
450000 　河南省郑州市，河南省人民医院，河南省立眼科医院，河南省眼科研究所（时倩倩，崔龙江）；450000　河南省郑州市，郑州人民医院（高彦，王李理）；450000　河南省郑州市，郑州大学第一附属医院（孙广莉）；450000　河南省郑州市，郑州大学药学院（陈秀英）；443003　河南省新乡市，新乡医学院第三附属医院眼科（李晓鹏）
Author(s):
SHI Qianqian1; GAO Yan2; WANG Lili2;  SUN Guangli3; CHEN Xiuying4; LI Xiaopeng5; CUI Longjiang1
1.Henan Eye Institute,Henan Eye Hospital,Henan Provincial People’s Hospital,Zhengzhou 450000,Henan Province
2.People’s Hospital of Zhengzhou,Zhengzhou 450000,Henan Province
3.The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,Henan Province
4.School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450000,Henan Province
5.Department of Ophthalmology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,Henan Province
关键词:
糖尿病视网膜病变; 杞菊地黄汤; 血管内皮生长因子; 缺氧诱导因子-1α
Keywords:
diabetic retinopathy;  Qiju Dihuang Decoction;  vascular endothelial growth factor;  hypoxia inducible factor-1α
分类号:
R774
DOI:
10.13389/j.cnki.rao.2020.0075
文献标志码:
A
摘要:
目的　观察杞菊地黄汤对糖尿病视网膜病变（DR）模型大鼠视网膜新生血管的作用及其机制。方法　采用STZ（40 mg·kg-1）诱导糖尿病大鼠模型，继续以高脂饲料饲养4周，眼底荧光素血管造影（FFA）观察大鼠视网膜病变情况确定DR模型是否成功。将DR大鼠（48只）分为模型组、羟苯磺酸钙组（5.4 mg·kg-1）、杞菊地黄汤高剂量组（16.74 g·kg-1)、低剂量组（8.37 g·kg-1），以正常大鼠为对照组（10只）。连续给药4周后，检测大鼠空腹血糖（FBG）和视网膜新生血管情况。ELISA法检测血清中血管生成素-1（Ang-1）和血管内皮生长因子（VEGF）的含量。Western-blot检测视网膜中缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子受体2（VEGFR2）的蛋白表达。RT-PCR法检测视网膜中Ang-1和VEGF mRNA的表达。结果　与对照组比较，模型组大鼠FBG显著升高（P<0.01），微血管数量增加、眼底荧光素渗漏，血清及视网膜中Ang-1、VEGF含量均升高（均为P<0.05），视网膜中HIF-1α和VEGFR2蛋白表达均显著升高（均为P<0.01）；与模型组比较，杞菊地黄汤高剂量组大鼠FBG下降（P<0.05），视网膜新生血管数量下降、荧光素渗漏面积显著减少，视网膜中Ang-1、VEGF、HIF-1α和VEGFR2水平均显著下降（均为P<0.05）；杞菊地黄汤低剂量组视网膜中HIF-1α、Ang-1、VEGF表达均下降（均为P<0.05）。结论　杞菊地黄汤能抑制DR模型大鼠新生血管，缓解视网膜病理损伤状况，其作用机制与调控HIF-1α/VEGF/VEGFR2信号有关。
Abstract:
Objective　To observe the effect of Qiju Dihuang Decoction on retinal angiogenesis in diabetic retinopathy (DR) model rats and to explore its mechanisms.Methods　Diabetic rat models were induced by STZ (40 mg·kg-1), and continued to be fed with high-fat diet for 4 weeks. Retinal lesions were observed by fundus fluorescein angiography (FFA) to determine whether the DR model was successful. Forty-eight DR rats were randomly divided into model group, calcium dobesilate (5.4 mg·kg-1) group, high-dose (16.74 g·kg-1) and low-dose (8.37 g·kg-1) of Qiju Dihuang Decoction, and other 10 normal rats were as controls. After 4 weeks of continuous administration, the fasting blood glucose (FBG) levels and retinal angiogenesis conditions were measured. The serum levels of angiopoietin-1 (Ang-1) and vascular endothelial growth factor (VEGF) were detected by ELISA. The expression of retinal hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor receptor 2 (VEGFR2) proteins was detected by Western-blot. The expression of retinal Ang-1 and VEGF mRNA was detected by RT-PCR.Results　Compared with the control group, FBG value in the model group significantly increased (P<0.01), the number of microvessels increased, the fundus fluorescein leakage was observed, and the levels of Ang-1 and VEGF in the serum and retina increased (all P<0.05). The expression of retinal HIF-1α and VEGFR2 proteins also significantly increased (both P<0.01). Compared with the model group, the FBG value decreased (P<0.05), the number of retinal neovascularization decreased, and the area of fluorescein leakage decreased significantly and the levels of Ang-1, VEGF, HIF-1α and VEGFR2 decreased in high-dose of Qiju Dihuang Decoction group (all P<0.05). Compared with the model group, the expression of HIF-1α, Ang-1 and VEGF decreased in the retina of low-dose of Qiju Dihuang Decoction group (all P<0.05).Conclusion　Qiju Dihuang Decoction can inhibit angiogenesis and relieve retinal pathological damage in DR model rats, and its mechanism is related to the regulation of HIF-1α/VEGF/VEGFR2 signaling.

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备注/Memo

备注/Memo:
河南省高等学校重点科研项目计划（编号：16B320017）
更新日期/Last Update: 2020-04-05