[1]于童,张劲松.Mfn2在TGF-β2诱导的晶状体上皮细胞上皮-间质转化中的作用[J].眼科新进展,2020,40(3):206-210.[doi:10.13389/j.cnki.rao.2020.0049]
　YU Tong,ZHANG Jinsong.Role of Mfn2 on TGF-β2-induced epithelial-mesenchymal transition of lens epithelial cells[J].Recent Advances in Ophthalmology,2020,40(3):206-210.[doi:10.13389/j.cnki.rao.2020.0049]
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Mfn2在TGF-β2诱导的晶状体上皮细胞上皮-间质转化中的作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
40卷
期数:
2020年3期
页码:
206-210
栏目:
实验研究
出版日期:
2020-03-05

文章信息/Info

Title:
Role of Mfn2 on TGF-β2-induced epithelial-mesenchymal transition of lens epithelial cells
作者:
于童; 张劲松
110005　辽宁省沈阳市，中国医科大学附属第四医院眼科，辽宁省晶状体学重点实验室(于童，张劲松)；110001　辽宁省沈阳市，沈阳爱尔卓越眼科医院（张劲松）
Author(s):
YU Tong1; ZHANG Jinsong1; 2
1.Department of Ophthalmology，the Fourth Affiliated Hospital of China Medical University，the Key Lenticular Laboratory of Liaoning Province，Shenyang 110005，Liaoning Province，China2.Shenyang Aier Excellence Eye Hospital，Shenyang 110001，Liaoning Province，China
关键词:
线粒体融合蛋白; 转化生长因子β2; 晶状体上皮细胞; 上皮-间质转化
Keywords:
mitochondrial fusion protein;  TGF-β2;  lens epithelial cells;  epithelial-mesenchymal transition
分类号:
R776.1
DOI:
10.13389/j.cnki.rao.2020.0049
文献标志码:
A
摘要:
目的　研究线粒体融合蛋白基因2（mitofusin 2，Mfn2）在转化生长因子β2（transforming growth factor β2，TGF-β2）诱导的人晶状体上皮细胞（human lens epithelial cells，HLECs）发生上皮-间质转化（epithelial-mesenchymal transition，EMT）过程中的表达及作用。方法　实时荧光定量PCR检测不同浓度（10 ng·mL-1、50 ng·mL-1、100 ng·mL-1）TGF-β2处理HLECs 24 h后，各TGF-β2处理组与对照组（0 ng·mL-1 TGF-β2） Mfn2 mRNA表达量差异。选取最佳作用浓度后，免疫荧光细胞化学检测TGF-β2处理组与对照组的Mfn2蛋白表达变化，实时荧光定量PCR和免疫荧光细胞化学检测分别验证TGF-β2处理组与对照组中E-钙黏蛋白（E-Cadherin）和波形蛋白（Vimentin） mRNA表达量和蛋白表达变化。Mfn2基因荧光表达载体（pEGFP-Mfn2）转染后实时荧光定量PCR和免疫荧光细胞化学检测分别验证转染组和对照组Mfn2、E-Cadherin和Vimentin mRNA表达量差异和蛋白表达变化。结果　TGF-β2处理组与对照组相比，E-Cadherin mRNA表达量减少；Vimentin、Mfn2的mRNA表达量均增加，且差异均有统计学意义（均为P<0.001）。E-Cadherin蛋白表达降低，Vimentin、Mfn2的蛋白表达增加。成功转染Mfn2后转染组与对照组相比，E-Cadherin mRNA表达量下调，Vimentin mRNA表达量均增加，差异均有统计学意义（均为P<0.001）。E-Cadherin蛋白表达降低，Vimentin蛋白表达增加。结论　HLECs在发生EMT时Mfn2表达量上调，过表达Mfn2可使HLECs发生EMT。Mfn2参与了TGF-β2诱导的HLECs的EMT过程。
Abstract:
Objective　To investigate the expression and role of mitofusin 2 (Mfn2) in the epithelial-mesenchymal transition (EMT) of human lens epithelial cells (HLECs) induced by transforming growth factor β2 (TGF-β2).Methods　Real-time fluorescence quantitative PCR is used to detect the difference in mRNA expression levels of Mfn2 between the TGF-β2 treated group and the control group (0 ng·mL-1) after HLECs was treated with TGF-β2 for 24 h at different concentrations (10 ng·mL-1, 50 ng·mL-1, 100 ng·mL-1). After the optimal concentration was selected, the Mfn2 protein expression in the TGF-β2 treated group and control group was detected by immunofluorescence cytochemistry. Real-time fluorescence quantitative PCR and immunofluorescence cytochemical detection were used to verify the changes in mRNA expression and protein expression of E-Cadherin and Vimentin in the TGF-β2 treated group and control group, respectively. After transfection of the Mfn2 gene fluorescent expression vector (pEGFP-Mfn2), the expression changes of mRNA and protein for Mfn2, E-Cadherin and Vimentin in the transfection group and the control group were verified by real-time quantitative PCR and immunofluorescence cytochemical detection, respectively.Results　Compared with the control group, the expression of E-Cadherin mRNA decreased in the TGF-β2 treated group; the expression levels of Vimentin and Mfn2 mRNA increased, and the differences were statistically significant (both P<0.001). The expression of E-cadherin decreased, and the expression of Vimentin and Mfn2 protein increased.Compared with the control group, the expression of E-Cadherin mRNA decreased and expression of Vimentin mRNA increased in transfection group after successful transfection of Mfn2, and statistical differences were found (both P<0.001). Expression of E-Cadherin protein decreased and Vimentin protein increased.Conclusion　The expression of Mfn2 is up-regulated when EMT occurs in HLECs. Overexpression of Mfn2 can induce EMT in HLECs. Mfn2 is involved in the EMT process of TGF-β2-induced HLECs.

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备注/Memo

备注/Memo:
国家自然科学基金项目（编号：81870644）
更新日期/Last Update: 2020-03-05