[1]王婧,刘新丽,吴慧茹,等.红芪多糖干预内毒素诱导的大鼠葡萄膜炎的机制[J].眼科新进展,2019,39(7):606-610.[doi:10.13389/j.cnki.rao.2019.0140]
　WANG Jing,LIU Xin-Li,WU Hui-Ru,et al.Mechanism of intervention of hedysarum polybotrys saccharide in endotoxin-induced uveitis in rats[J].Recent Advances in Ophthalmology,2019,39(7):606-610.[doi:10.13389/j.cnki.rao.2019.0140]
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
39卷
期数:
2019年7期
页码:
606-610
栏目:
实验研究
出版日期:
2019-07-05

文章信息/Info

Title:
Mechanism of intervention of hedysarum polybotrys saccharide in endotoxin-induced uveitis in rats
作者:
王婧; 刘新丽; 吴慧茹; 贾辉钰; 卢弘
100020　北京市，首都医科大学附属北京朝阳医院眼科（王婧，卢弘）；100080　北京市，海淀妇幼保健院眼科（刘新丽）；730000　甘肃省兰州市，兰州大学第一医院眼科（吴慧茹，贾辉钰）
Author(s):
WANG Jing; LIU Xin-Li; WU Hui-Ru; JIA Hui-Yu; LU Hong
Department of Ophthalmology,Chaoyang Hospital,Capital Medical University(WANG Jing,LU Hong),Beijing 100020，China;Department of Ophthalmology,Haidian Maternity and Child Health Hospital(LIU Xin-Li),Beijing 100080,China;Department of Ophthalmology,the First Hospital of Lanzhou University (WU Hui-Ru,JIA Hui-Yu),Lanzhou 730000,Gansu Province,China
关键词:
红芪多糖; 内毒素诱导的葡萄膜炎; 肿瘤坏死因子受体相关因子6; TIR功能区接头蛋白诱导的干扰素β
Keywords:
hedysarum polybotrys saccharide; endotoxin-induced uveitis; tumor necrosis factor receptor-associated factor 6; TIR-domain-containing adapter-inducing interferon-β
分类号:
R773
DOI:
10.13389/j.cnki.rao.2019.0140
文献标志码:
A
摘要:
目的　探讨红芪多糖（hedysarum polybotrys saccharide,HPS）在内毒素诱导的大鼠葡萄膜炎发病中的作用及对Toll样受体4（Toll-like receptor 4,TLR-4）信号转导通路中关键分子肿瘤坏死因子受体相关因子6（tumor necrosis factor receptor-associated factor 6，TRAF6）及TIR功能区接头蛋白诱导的干扰素β（TIR-domain-containing adapter-inducing interferon-β，TRIF)表达的影响。方法　选用SPF级健康成年Wistar大鼠40只，随机分为内毒素诱导的葡萄膜炎（endotoxin induced uveitis,EIU）模型组、HPS组、HPS+内毒素组、正常对照组（normal control，NC）组，每组10只。EIU模型组大鼠采用皮下注射1 mg·kg-1内毒素的方法建立大鼠急性前葡萄膜炎动物模型。HPS+内毒素组在内毒素注射前1 h给予腹腔注射HPS 400 mg·kg-1。HPS组大鼠只注射HPS 400 mg·kg-1。每2 h用裂隙灯观察大鼠眼前节炎症反应，注射后的0 h、6 h、12 h、18 h、24 h对各组眼部临床炎症反应进行评分。注射后24 h组织病理学检查炎症反应水平。RT-PCR检测核因子-κB、TRAF6及TRIF mRNA的表达。结果　NC组大鼠24 h内眼前节未见炎症反应。注射后24 h,EIU模型组可见虹膜血管扩张明显，瞳孔区大量白色渗出,HPS组仅可见虹膜血管扩张，未见其他眼部炎症反应,HPS+内毒素组大鼠可见虹膜血管扩张和瞳孔缘少量渗出。比较不同组间的大鼠眼部临床炎症反应评分可见，NC组与EIU模型组、HPS+内毒素组、HPS组比较，差异均有统计学意义(均为P＜0.001）；EIU模型组与HPS+内毒素组、HPS组比较，差异均有统计学意义（均为P<0.05）;HPS+内毒素组与HPS组比较，差异有统计学意义（P＜0.001）。RT-PCR结果显示，HPS可降低核因子-κB mRNA和TRAF6 mRNA的表达（P＜0.001），HPS对TRIF的表达无明显影响（P=0.236）。结论　HPS可能通过抑制TRAF6核酸的表达缓解EIU的炎症反应,HPS对TRIF的表达无明显影响。
Abstract:
Objective　To investigate the effect of hedysarum polybotrys saccharide (HPS) on endotoxin-induced uveitis(EIU) in rats and to explore the effect on the expression of tumor necrosis factor receptor-associated factor 6 (TRAF6 )and TIR-domain-containing adapter-inducing interferon-β(TRIF )in the Toll-like receptor 4(TLR-4) signal transduction pathway in EIU.Methods　Experiment select the SPF healthy adult Wistar rats,40 rats were randomly divided into four groups.EIU group,hedysarum polybotrys saccharide treatment (HPS+LPS) group,hedysarum polybotrys saccharide control (HPS) group,normal control (NC) group.Ten rats in each group.EIU was established by subcutaneous injection of 1 mg·kg-1 lipopolysaccharide (LPS).HPS (400 mg·kg-1) was given through intraperitoneal injection 1 h before the LPS induction in HPS treatment group.Rats in the HPS control group were only injected with 400 mg·kg-1 of HPS.The inflammation of rats was observed every 2 h with slit lamp,and each group was graded at 0 h,6 h,12 h,18 h and 24 h after injection.Histopathological examination of inflammatory was performed 24 h after injection.Real-time quantitative PCR were used to determine the expression of nuclear factor-kappa B(NF-κB）,TRAF6 and TRIF.Results　No inflammation was observed in NC group within 24 h.24 h after injection,iris blood vessel dilation and a large amount of white exudation was observed in the pupil area in the EIU group.Only iris blood vessel dilation was observed in the HPS group.In the HPS+LPS group,iris blood vessel dilation and a small amount of exudation in the pupil margin were observed.Compared clinical scores of rats in different groups,and the differences were statistically significant among all groups (all P<0.001).Compared with HPS+LPS group and HPS group,the difference of EIU group was statistically significant (both P<0.05).The difference of inflammation between the LPS+HPS group and the HPS group was statistically significant (P<0.001).Real-time quantitative PCR results showed that HPS could reduce the expression of NF-κB and TRAF6 mRNA (both P<0.001),while HPS had no significant effect on the expression of TRIF (P=0.236).Conclusion　HPS can restrain the intraocular inflammation in EIU by inhibiting the expression of TRAF6 mRNA.HPS has no significant effect on the expression of TRIF.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：81273246、81471575）；甘肃省自然科学基金资助（编号：054000105）
更新日期/Last Update: 2019-07-01