[1]宋德胜,钱晶,陈志钧.艾芬地尔对单眼形觉剥夺小鼠初级视皮层兴奋性递质受体表达的影响[J].眼科新进展,2019,39(4):326-330.[doi:10.13389/j.cnki.rao.2019.0073]
　SONG De-Sheng,QIAN Jing,CHEN Zhi-Jun.Ifenprodil on the expression of excitability neurotransmitter receptors following monocular form deprivation in visual cortex of mice[J].Recent Advances in Ophthalmology,2019,39(4):326-330.[doi:10.13389/j.cnki.rao.2019.0073]
点击复制

艾芬地尔对单眼形觉剥夺小鼠初级视皮层兴奋性递质受体表达的影响/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
39卷
期数:
2019年4期
页码:
326-330
栏目:
实验研究
出版日期:
2019-04-05

文章信息/Info

Title:
Ifenprodil on the expression of excitability neurotransmitter receptors following monocular form deprivation in visual cortex of mice
作者:
宋德胜; 钱晶; 陈志钧
210029　江苏省南京市，南京医科大学附属儿童医院眼科
Author(s):
SONG De-Sheng; QIAN Jing; CHEN Zhi-Jun
Department of Ophthalmology，Children’s Hospital of Nanjing Medical University，Nanjing 210029，Jiangsu Province，China
关键词:
NR2B; 初级视皮层; 艾芬地尔; 单眼形觉剥夺
Keywords:
NR2B; visual cortex; ifenprodil; monocular deprivation
分类号:
R777.4
DOI:
10.13389/j.cnki.rao.2019.0073
文献标志码:
A
摘要:
目的　检测N-甲基-D-天冬氨酸 （N-mehyl-D-aspartate，NMDA）受体亚基NR2B （NMDA-NR2B）阻断剂艾芬地尔对单眼形觉剥夺小鼠初级视皮层内兴奋性递质受体亚基关键期相对表达量的影响，为弱视机制研究提供实验基础。方法　选取出生后3~4周健康C57BL/6J小鼠15只，随机分为正常对照组、单眼形觉剥夺组、联合组，每组5只。单眼形觉剥夺组和联合组小鼠右眼褥式缝合6 d，建立单眼形觉剥夺模型，联合组于第1、3、5天腹腔注射艾芬地尔，给药量为10 mg·kg-1。采用qRT-PCR法检测各组小鼠视皮层NR2A mRNA和NR2B mRNA的相对表达量；采用Western blot法检测各组小鼠视皮层NR2A蛋白和NR2B蛋白的相对表达情况。结果　与正常对照组相比，单眼形觉剥夺组小鼠左侧视皮层NR2A mRNA和蛋白表达下降，差异均有统计学意义（均为P＜0.05），NR2B mRNA和蛋白表达升高，差异均有统计学意义（均为P＜0.05）；右侧视皮层NR2A mRNA和蛋白表达升高，差异均有统计学意义（均为P＜0.05），NR2B mRNA和蛋白表达下降，差异均有统计学意义（均为P＜0.05）。联合组、单眼形觉剥夺组小鼠左侧视皮层NR2A mRNA和蛋白相对表达量分别为0.99±0.22、0.75±0.08和0.58±0.44、0.50±0.36，差异均无统计学意义（均为P>0.05）；联合组小鼠左侧视皮层NR2B蛋白相对表达量为1.79±0.84，低于单眼形觉剥夺组小鼠（4.83±2.06），差异有统计学意义（P＜0.05）。结论　NR2B受体阻断剂艾芬地尔能显著降低NR2B亚基的表达，并阻断单眼形觉剥夺对NR2B基因及蛋白表达的影响，但对NR2A基因及蛋白表达无影响。
Abstract:
Objective　To investigate the effects of ifenprodil on the expression of N-methy-D-aspartate （NMDA） receptor subunit NR2B （NMDA- NR2B） following monocular form deprivation in visual cortex of mice during the critical period of visual development for providing theoretical evidences for amblyopia.Methods　Totally 15 healthy aged 3-5 weeks C57BL/6J mice were randomly divided into normal control group，monocular form deprivation group and the combined group，5 cases in each group.The right eyes of these mice in the monocular form deprivation group and the combined group was sutured for 6 days to establish the model of monocular form deprivation，while the mice in the combined with intraperitoneal injection group were given unilateral intraperitoneal injection of 10 mg·kg-1 ifenprodil on day 1，3 and 5.qRT-PCR，Western blot were used to detect the mRNA and protein expression of NMDA receptor subunits NR2A and NR2B.Results　The expression levels of NR2A mRNA and protein in the left visual cortex of the monocular form deprivation group were lower，but the levels of NR2B mRNA and protein expression were higher as compared with the normal control group（all P<0.05）.The levels of NR2A mRNA and protein in the right visual cortex of the monocular form deprivation group，but the levels of NR2B mRNA and protein were lower than those of the normal control group（all P<0.05）.The NR2A mRNA，protein in the left visual cortex of the combined group and monocular form deprivation group was 0.99±0.22，0.75±0.08 and 0.58±0.44，0.50±0.36，and the differences were not statistically significant（all P>0.05）.The NR2B protein in the left visual cortex of the combined group was 1.79±0.84，which was lower than of the monocular deprivation group （4.83±2.06），and the difference was statistically significant（P<0.05）.Conclusion　NR2B subunit inhibitor Ifenprodil could suppress the expression of NR2B protein，and eliminate the effect of monocular deprivation on the expression of NR2B subunit，but it had no effect on the expression of NR2A subunit.

参考文献/References:

［1］　ZHAO K X，ZHENG Y Z.Problems to be resolved urgently on amblyopia diagnosis and prevention in China［J］.Chin J Ophthalmol，2009，45（11）：961-962.
赵堪兴，郑曰忠.目前我国弱视临床防治中亟待解决的问题［J］.中华眼科杂志，2009，45（11）：961-962.
［2］　KIRKWOOD A，BEAR M F.Hebbian synapses in visual cortex［J］.J Neurosci，1994，47（14）：1634-1645.
［3］　TIGARET C M，OLIVO V，SADOWSKI J H，ASHBY M C，MELLOR J R.Coordinated activation of distinct Ca2+" sources and metabotropic glutamate receptors encodes Hebbian synaptic plasticity［J］.Nat Commun，2016，7：10289.
［4］　BARNES S J，SAMMONS R P，JACOBSEN R I，MACKIE J，KELLER G B，KECK T.Subnetwork-specific homeostatic plasticity in mouse visual cortex in vivo［J］.Neuron，2015，86（5）：1290-1303.
［5］　GUO Y T，HUANG S Y，DE P R，MCGEHRIN K，LEE H K，ZHAO K，et al.Dark exposure extends the integration window for spike-timing-dependent plasticity［J］.J Neurosci，2012，32（43）：15027-15035.
［6］　CAO Z，LIU L，LICKEY M，GRAVES A，PHAM T，GORDON B.Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity［J］.Exp Brain Res，2015，177（1）：64-77.
［7］　HENSCH T K.Critical period plasticity in local cortical circuits［J］.Nat Rev Neurosci，2005，6（11）：877-888.
［8］　KARMARKAR R，DAN Y.Experience-dependent plasticity in adult visual cortex［J］.Neuron，2006，52（4）：577-585.
［9］　DEIDDA G，ALLEGRA M，CERRI C，NASKAR S，BONY G，ZUNINO G，et al.Early depolarizing GABA controls critical-period plasticity in the rat visual cortex［J］.Nat Neurosci，2015，18（1）：87-96.
［10］　CUI Z，FENG R，JACOBS S，DUAN Y，WANG H，CAO X，et al.Increased NR2A：NR2B ratio compresses long-term depression range and constrains long-term memory［J］.Sci Rep，2013，3：1036.
［11］　GUO Y T，HUANG S Y，DE P R，MCGEHRIN K，LEE H K，ZHAO K，et al.Dark exposure extends the integration window for spike-timing-dependent plasticity［J］.J Neurosci，2012，32（43）：15027-15035.
［12］　MONTERO V M.Amblyopia decreases activation of the corticogeniculate pathway and visual thalamic reticularis in attentive rats：afocal attention hypothesis［J］.Neuroscience，1999，91（3）：805-817.
［13］　GORDON J A，STRYKER M P.Experience-dependent plasticity of binocular responses in the primary visual cortex of the mouse［J］.J Neurosci，1996，16（10）：3274-3286.
［14］　MAHMOUD G S，AMER A S.Co-Application of Corticosterone and Growth Hormone Upregulates NR2B Protein and Increases the NR2B：NR2A Ratio and Synaptic Transmission in the Hippocampus［J］.Sultan Qaboos Univ Med J，2014，14（4）：e486-494.
［15］　LI Y H，WANG J，ZHANG G.Involvement of synaptic NR2B-containing NMDA receptors in long-term depression induction in the young rat visual cortex in vitro［J］.Chin J Physiol，2011，54（3）：190-195.
［16］　DE M J，ROBERTS A C，MIDDLEBROOKS P G，VALAKH V，YASHIRO K，WILFLEY L R，et al.NMDA receptor antagonists reveal age-dependent differences in the properties of visual cortical plasticity［J］.J Neurophysiol，2008，100（4）：1936-1948.
［17］　NASE G，WEISHAUPT J，STEMERN P，SINGER W，MONYER H，et al.Genetic and epigenetic regulation of NMDA receptor expression in the rat visual cortex［J］.Eur J Neurosci，1999，11（12）：4320-4326.
［18］　LIU X B，MURRAY K D，JONES E G.Switching of NMDA receptor 2A and 2B subunits at thalamic and cortical synapses during early postnatal development［J］.J Neurosci，2004，24（40）：8885-8895.
［19］　HOFFMANN H，GREMME T，HATT H，GOTTMANN K.Synaptic activity dependent developmental regulation of NMDA receptor subunit expression in cultured neocortical neurons［J］.J Neurochem，2000，75（4）：1590-1599.
［20］　JURD R，THORNTON C，WANG J，LUONG K，PHAMLUONG K，KHARAZIA V，et al.Mind bomb-2 is an E3 ligase that ubiquitinates the N-methyl-D-aspartate receptor NR2B subunit in a phosphorylation- dependent manner［J］.J Biol Chem，2008，283（1）：301-310.
［21］　QUINLAN E M，OLSTEIN D H，BEAR M F.Bidirectional，experiencedependent regulation of N-methyl-D-aspartate receptor subunit composition in the rat visual cortex during postnatal development［J］.Proc Natl Acad Sci U S A，1999，96（22）：12876-12880.
［22］　HALEY J E，DICKENSON A H.Evidence for spinal N-methyl-D-aspartate receptor involvement in prolonged chemical nociception in the rat［J］.Brain Res，2016，96（22）：58-60.
［23］　VOROBEYCHIK Y，WILLOUGHBY C D，MAO J.NMDA Receptor Antagonists in the Treatment of Pain［M］//Treatment of Chronic Pain by Medical Approaches.Springer：New York，2015，24（40）：59-65.
［24］　BURNASHEV N，SZEPETOWSKI P.NMDA receptor subunit mutations in neurodevelopmental disorders［J］.Curr Opin Pharmacol，2015，20（34）：73-82.

相似文献/References:

[1]冯虹 卢艳.视野前青光眼的不同改变及诊断研究进展[J].眼科新进展,2013,33(11):000.
[2]林玲,任京力. 胆碱能神经元在闪光刺激增强大鼠初级视皮层可塑性中的作用[J].眼科新进展,2015,35(3):231.[doi:10.13389/j.cnki.rao.2015.0062]
　LIN Ling,REN Jing-Li. Effects of cholinergic neurons in flashing stimulation enhanced plasticity of primary visual cortex in rats[J].Recent Advances in Ophthalmology,2015,35(4):231.[doi:10.13389/j.cnki.rao.2015.0062]
[3]张秀艳,毕爱玲,吴姗姗,等.电针刺激对单眼形觉剥夺弱视大鼠初级视皮层中多巴胺、γ-氨基丁酸及其受体mRNA表达的影响[J].眼科新进展,2020,40(12):1106.[doi:10.13389/j.cnki.rao.2020.0246]
　ZHANG Xiuyan,BI Ailing,WU Shanshan,et al.Effect of electroacupuncture on expression of dopamine, γ-aminobutyric acid and its receptor mRNA in primary visual cortex of monocular deprivation amblyopic rats[J].Recent Advances in Ophthalmology,2020,40(4):1106.[doi:10.13389/j.cnki.rao.2020.0246]

备注/Memo

备注/Memo:
江苏省青年医学人才（QNRC2016080）
更新日期/Last Update: 2019-04-15