CHEN Yu-Qing,JIAN Shou-Jun,YUE Juan,et al.The effect of high-fructose diet on the course of fungal keratitis in mice[J].Recent Advances in Ophthalmology,2019,39(4):301-305.[doi:10.13389/j.cnki.rao.2019.0068]





The effect of high-fructose diet on the course of fungal keratitis in mice
450003 河南省郑州市,郑州大学人民医院,河南省立眼科医院,河南省眼科研究所,河南省人民医院,河南省眼科学与视觉科学重点实验室(陈雨晴,简守珺,岳娟,刘素素,王丽娅);510632 广东省广州市,暨南大学医学院眼科研究所,暨南大学再生医学教育部重点实验室(李志杰)
CHEN Yu-QingJIAN Shou-JunYUE JuanLIU Su-SuLI Zhi-JieWANG Li-Ya
People’s Hospital of Zhengzhou University,Henan Eye Hospital,Henan Eye Institute,Henan Provincial People’s Hospital,Henan Key Laboratory of Ophthalmology and Vision Science(CHEN Yu-Qing,JIAN Shou-Jun,YUE Juan,LIU Su-Su,WANG Li-Ya),Zhengzhou 450003,Henan Province,China;Institute of Ophthalmology,Medical School of Jinan University,Key Laboratory for Regenerative Medicine,Ministry of Education,Jinan University(LI Zhi-Jie),Guangzhou 510632,Guangdong Province,China
high-fructose dietfungal keratitisneutrophilmacrophage
目的 探究高糖饮食对小鼠真菌性角膜炎的影响。方法 选取健康无眼疾的雄性C57BL/6J小鼠78只,随机分为高糖饮食组和模型对照组,每组36只,模型对照组给予正常饮用水,高糖饮食组给予含体积分数10%果糖溶液,每2 d测量两组小鼠体质量及血糖,10 d后建立真菌性角膜炎模型。造模后24 h、36 h、48 h、72 h、96 h、120 h、168 h裂隙灯显微镜下对角膜进行临床评分并拍照。处死小鼠后,取角膜组织进行HE染色和PAS染色;测定角膜内中性粒细胞和巨噬细胞浸润体积。利用酶联免疫吸附实验对小鼠角膜内的白细胞介素-1β含量进行测定。结果 造模后 0~14 d,两组小鼠体质量与血糖差异均无统计学意义(均为P>0.05)。造模后24 h、36 h、48 h、120 h、168 h,高糖饮食组小鼠角膜临床评分均明显高于模型对照组,差异均有统计学意义(均为P<0.05)。高糖饮食组小鼠角膜穿孔率79.5%,高于模型对照组的40.9%,差异有统计学意义(P=0.000)。造模后各时间点,高糖饮食组中性粒细胞浸润体积均高于模型对照组,差异均有统计学意义(均为P=0.000)。造模后72 h、96 h、120 h、168 h,高糖饮食组巨噬细胞浸润体积均高于模型对照组(均为P=0.000)。角膜组织病理学检查结果示,高糖饮食组炎症反应更重,角膜组织破坏更早且更为严重。造模后24 h、48 h高糖饮食组白细胞介素-1β含量均明显高于模型对照组(均为P<0.05)。结论 高糖饮食加重了真菌性角膜炎感染的严重程度,增强了中性粒细胞、巨噬细胞的趋化,促进了IL-1β的分泌。
Objective To investigate and observe the effect of high-fructose diet on the course of fungal keratitis (FK) in mice.Methods Totally 78 C57BL/6 male mice (free of eye diseases) were distributed into high-fructose diet group and control group randomly,and each group had 39 mice.The high-fructose diet group was provided with a 10% fructose solution,and control group was given potable water.We measured the weight and blood glucose of the two groups every other day.After 10 days,we established the FK model.At 24 h,36 h,48 h,72 h,96 h,120 h,168 h after modeling,we assessed the clinical manifestations and took photos under slit-lamp microscope.After mice were euthanized,HE staining and PAS staining were performed to the cornea tissues.We analyzed the volumes of neutrophils and macrophages in the cornea.Enzyme-linked immunosorbent assay was used to detect interleukin-1β (IL-1β) content in the cornea.Results At 0-14 days after modeling,the difference of weight and blood glucose of two groups mice was not statistically significant(P>0.05).At 24 h,36 h,48 h,120 h,168 h after modeling,the clinical scores of the high-fructose diet group were significantly higher as comparison with the control group at each time point,and the differences were statistically significant (all P<0.05).Corneal perforation rate was 79.5% in the high-fructose diet group,while 40.9% in the control group,and the difference was statistically significant(P=0.000).The volume of neutrophil was higher in the high-fructose diet group than in the control group at each time point after modeling,and the difference among two groups was statistically significant(all P=0.000).At 72 h,96 h,120 h,168 h after modeling,the volume of macrophage was higher in the high-fructose diet group than in the control group (all P=0.000).Corneal histopathological examination showed that,in the high-fructose diet group,the inflammation reaction was more severe,and the corneal tissue destruction occurred earlier and more severe.The content of IL-1β in high-fructose group was higher than that in the control group at 24 h,48 h after modeling (all P<0.05).Conclusion High-fructose diet aggravates the severity of FK infection,enhances the chemotaxis of neutrophils and macrophages and promotes the secretion of IL-1β.


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更新日期/Last Update: 2019-04-15