[1]张园园,黄婷婷,卢悦,等.香椿叶提取物对高脂大鼠视网膜组织保护作用的研究[J].眼科新进展,2018,38(4):329-333.[doi:10.13389/j.cnki.rao.2018.0077]
　ZHANG Yuan-Yuan,HUANG Ting-Ting,LU Yue,et al.Protective effects of Toona sinensis leaf extract on retinal tissue in rats with hyperlipidemia[J].Recent Advances in Ophthalmology,2018,38(4):329-333.[doi:10.13389/j.cnki.rao.2018.0077]
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香椿叶提取物对高脂大鼠视网膜组织保护作用的研究/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年4期
页码:
329-333
栏目:
实验研究
出版日期:
2018-04-05

文章信息/Info

Title:
Protective effects of Toona sinensis leaf extract on retinal tissue in rats with hyperlipidemia
作者:
张园园; 黄婷婷; 卢悦; 刘艳丽; 于利; 刘华
121000　辽宁省锦州市，锦州医科大学（张园园，黄婷婷，卢悦，刘艳丽）；121000　辽宁省锦州市，锦州医科大学基础医学院生理教研室，辽宁省年龄相关性黄斑变性防治重点实验室（于利）；121000　辽宁省锦州市，辽宁省锦州医科大学眼科研究所，辽宁省年龄相关性黄斑变性防治重点实验室（刘华）
Author(s):
ZHANG Yuan-Yuan; HUANG Ting-Ting; LU Yue; LIU Yan-Li; YU Li; LIU Hua
Jinzhou Medical University,Jinzhou 121000,Liaoning Province;Department of Physiology and Research,School of Basic Medicine,Jinzhou Medical University,Key Laboratory for Prevention and Control of Age-related Macular Degeneration in Liaoning,Jinzhou 121000,Liaoning Province;Institute of Ophthalmology,Jinzhou Medical University,Key Laboratory for Prevention and Control of Age-related Macular Degeneration in Liaoning Province,Jinzhou 121000,Liaoning Province
关键词:
年龄相关性黄斑变性; 脂代谢异常; 香椿叶提取物; B淋巴细胞瘤-2; Bcl-2相关x蛋白
Keywords:
age-related macular degeneration; lipid metabolism disorder; toona sinensis leaf extract; Bcl-2; Bax
分类号:
R774.1
DOI:
10.13389/j.cnki.rao.2018.0077
文献标志码:
A
摘要:
目的　探讨香椿叶提取物（toona sinensis leaf extract，TSLE）对高脂大鼠视网膜组织的保护作用及对B淋巴细胞瘤-2（B-cell lymphoma-2，Bcl-2）蛋白和Bcl-2相关x蛋白（Bcl-2 associated x protein，Bax）表达的影响。方法　雄性SD大鼠24只，随机分为正常组（N组）、高脂模型组（HF组）和高脂模型+TSLE组（HF+TSLE组），后两组高脂饮食诱导高脂血症模型；8周后确认造模成功，HF+TSLE组给予TSLE水溶液灌胃4周，N组和HF组给予相同剂量生理盐水。12周末对大鼠进行视觉电生理、血清血脂总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）、低密度脂蛋白-胆固醇（low density lipoprotein-cholesterol，LDL-C）和高密度脂蛋白-胆固醇（high density lipoprotein-cholesterol，HDL-C）检测，HE染色，免疫组织化学和Western bloting检测Bcl-2蛋白、Bax蛋白的表达，分析凋亡蛋白Bax的表达水平与电生理功能异常的相关性。结果　HF组TC、TG、LDL-C较N组高，HDL-C降低，差异均有统计学意义（均为P<0.05）；HF+TSLE组TC、TG、LDL-C较HF组降低，HDL-C明显升高，差异均有统计学意义（均为P<0.05）；HF+TSLE组HDL-C较N组降低，TC、TG、LDL-C较N组增加，差异均无统计学意义（均为P>0.05）。三组大鼠a波潜伏期比较，HF组较N组延长，HF+TSLE组较HF组缩短，差异均有统计学意义（均为P<0.05）；HF+TSLE组较N组延长，差异无统计学意义（P>0.05）。三组大鼠b波潜伏期差异无统计学意义（P>0.05）；三组a波、b波振幅差异均无统计学意义（均为P>0.05）。HF组Bcl-2蛋白表达水平表达水平较N组显著降低，Bax蛋白较N组升高；HF+TSLE组较HF组Bcl-2蛋白表达水平升高、Bax蛋白表达明显下降。各组Bax蛋白表达水平与a波和b波潜伏期均呈正相关（均为P<0.05），与a波和b波振幅均无相关性（均为P>0.05）。结论　TSLE对脂代谢异常大鼠视网膜具有保护作用，并且可能是通过调节Bcl-2蛋白、Bax蛋白的表达来发挥作用的。
Abstract:
Objective　To investigate the protective effects of Toona sinensis leaf extract on the retina of rats with high-fat diet and the expression of B-cell lymphoma (Bcl-2) and Bcl-2 associated x protein (Bax).Methods　Together 24 male SD rats were randomly divided into normal group (N group)，hyperlipidemia model group (HF group) and hyperlipidemia model + toona sinensis leaf extract (HF+TSLE group)，and then hyperlipidemia model was induced by fed high-fat diet in the latter two groups；after 8 weeks，the model was confirmed to be successful，and the rats in HF+TSLE group were fed with TSLE solution for 4 weeks continuously，and rats in N group and HF group were given the same dose of physiological saline.At the end of the twelfth week，all rats were followed by the examination of flash electroretinogram (FERG)，serum lipid total cholesterol (TC)，triglyceride (TG)，low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C).Then，HE staining was performed in the retinas，the expression of Bcl-2 and Bax was detected by immunohistochemistry and Western blotting for the analysis of the correlation between the expression level of apoptotic protein Bax and the abnormal function of FERG.Results　In HF group，the content of HDL-C decreased，and the contents of TC，TG and LDL-C were higher than those in N group，and the differences were statistically significant (all P<0.05).The contents of TC，TG and LDL-C in HF+TSLE group were lower than those in HF group，but the content of HDL-C was significantly increased，and the differences were statistically significant (all P<0.05).The content of HDL-C in HF+TSLE group was lower than that in N group，while the contents of TC，TG and LDL-C were higher than those in N group，and the differences were not statistically significant (all P>0.05).The difference of a wave latency between the three groups was statistically significant (P<0.05)，and the latency of a wave in HF group was longer than that in N group，while HF+TSLE group was shorter than HF group，and the difference was statistically significant (P<0.05)，but HF+TSLE group was longer than N group，and the difference was not statistically significant (P>0.05).Moreover，there was no significant difference in the incubation period of b wave in the three groups (P>0.05)；and there was no significant difference in the amplitude of a wave and b wave in the three groups (all P>0.05).In addition，HF group had lower expression level of Bcl-2 and overexpression of Bax than N group.The expression level of Bcl-2 increased and Bax expression level decreased significantly in HF+TSLE group，and the expression level of Bax was positively correlated with the latency of a wave and b wave (all P<0.05)，but was not correlated with amplitude of a wave and b wave (all P>0.05).Conclusion　TSLE has an important role in the retina of rats with abnormal lipid metabolism，and it may play a protective role by regulating the expression of Bcl-2 and Bax.

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备注/Memo

备注/Memo:
辽宁省自然科学基金－锦州医科大学联合基金（编号：20170540399）；辽宁省科学技术基金［面上项目（原优秀人才培育）］（编号：201602298）
更新日期/Last Update: 2018-04-02