[1]杜倩,侯旭,李剑波,等.白皮杉醇对青光眼大鼠视网膜神经节细胞的保护作用[J].眼科新进展,2017,37(6):527-530.[doi:10.13389/j.cnki.rao.2017.0133]
　DU Qian,HOU Xu,LI Jian-Bo,et al.Protective effects of piceatannol on retinal ganglion cells in experimental glaucoma rats[J].Recent Advances in Ophthalmology,2017,37(6):527-530.[doi:10.13389/j.cnki.rao.2017.0133]
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白皮杉醇对青光眼大鼠视网膜神经节细胞的保护作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年6期
页码:
527-530
栏目:
实验研究
出版日期:
2017-06-05

文章信息/Info

Title:
Protective effects of piceatannol on retinal ganglion cells in experimental glaucoma rats
作者:
杜倩; 侯旭; 李剑波; 周蜂人; 杜琛
473000　河南省南阳市，南阳市中心医院眼科（杜倩，李剑波，周蜂人）；710032　陕西省西安市，第四军医大学西京医院眼科（侯旭）；411300　河南省南阳市，南阳市唐河县城郊医院公共卫生服务科（杜琛）
Author(s):
DU Qian; HOU Xu; LI Jian-Bo; ZHOU Feng-Ren; DU Chen
Department of Ophthalmology，Nanyang Central Hospital(DU Qian，LI Jian-Bo，ZHOU Feng-Ren)，Nanyang 473000，Henan Province，China；Department of Ophthalmology，Xijing Hospital，the Fourth Military Medical University(HOU Xu)，Xi’an 710032，Shaanxi Province，China；Department of Public Health Services，Tanghe Suburban Hospital(DU Chen)，Nanyang 411300，Henan Province，China
关键词:
白皮杉醇; 视网膜神经节细胞; MARK信号通路
Keywords:
piceatannol; retinal ganglion cells; MARK signaling pathway
分类号:
R774.1
DOI:
10.13389/j.cnki.rao.2017.0133
文献标志码:
A
摘要:
目的　观察白皮杉醇对青光眼大鼠视网膜神经节细胞（retinal ganglion cells，RGCs）的保护作用并探讨其可能的机制。方法　将40只大鼠随机分为对照组、模型组、白皮杉醇低剂量组和白皮杉醇高剂量组。采用光凝法建立青光眼大鼠模型，白皮杉醇低、高剂量组分别给予100 mg·kg-1和200 mg·kg-1白皮杉醇灌胃。测量各组大鼠眼压；荧光金逆行标记各组大鼠RGCs并计数；HE染色观察各组大鼠视网膜组织病理形态；免疫印迹法检测各组大鼠视网膜组织中磷酸化原癌基因蛋白Jun（proto oncogene protein Jun，c-Jun）氨基末端激酶（phosphorylation c-Jun N-terminal kinase，p-JNK）、磷酸化c-Jun（phosphorylation c-jun，p-c-Jun）、磷酸化细胞外调节蛋白激酶(phosphorylation extracellular regulated protein kinases，p-ERK)、磷酸化丝裂原活化蛋白激酶p38（phosphorylation mitogen activated protein kinases p38，p-p38 MAPK)和肿瘤坏死因子-α（tumor necrosis factor-alpha，TNF-α)蛋白表达。结果　模型组眼压、p-JNK、p-c-Jun、p-ERK、p-p38 MAPK和TNF-α蛋白表达均显著高于对照组（均为P<0.05），RGCs低于对照组（P<0.05），视网膜组织结构可见空泡和水肿样改变。与模型组相比，白皮杉醇低剂量组和白皮杉醇高剂量组大鼠RGCs显著增多（P=0.003、P=0.002），视网膜组织水肿和空泡样状况改善，p-JNK、p-c-Jun、p-ERK、p-p38 MAPK和TNF-α蛋白表达明显减少（均为P<0.05），且高剂量组的改善作用更为显著。结论　白皮杉醇具有保护青光眼大鼠RGCs的作用，其作用机制可能与抑制MARK信号通路有关。
Abstract:
Objective　To investigate the protective effects of piceatannol on retinal ganglion cells (RGCs) in rats with glaucoma.Methods　Forty rats were randomly divided into 4 groups：control group，model group，low dose piceatannol treatment group and high dose piceatannol treatment group.Photocoagulation method was used to establish the experimental glaucoma model in rats，and then rats were given 100 mg·kg-1 or 200 mg·kg-1 of piceatannol by gavage.The intraocular pressure was measured before and after the model was established.Rat retinal ganglion cells were labeled and counted using FG staining.Retinal tissue pathological morphology was observed by HE staining.The protein expression of p-JNK，p-c-Jun，p-ERK，p-p38 MAPK and TNF-α were measured by western blot.Results　Compared with control group，the intraocular pressure，the protein expressions of p-JNK，p-c-Jun p-ERK，p-p38 MAPK and TNF-α were significantly increased in model group (all P<0.05).However，the number of RGCs were lower in model group（P<0.05）.Furthermore，there were cavitation and edema changes in retinal tissue of model group.Compared with model group，piceatannol treatment markedly increased the number of RGCs（P=0.003，0.002），improved the pathological morphology of retinal tissue，and reduced the protein expressions of p-JNK，p-c-Jun p-ERK，p-p38 MAPK and TNF-α (all P<0.05)，especially for the high concentration.Conclusion　Piceatannol can protect against RGCs injury in glaucoma rats，and the mechanism may be associated with inhibition of MARK signaling pathway.

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更新日期/Last Update: 2017-06-28