[1]李兰根,伟伟,张玉凤,等.信号转导/转录激活因子3抗ARPE-19细胞氧化应激研究[J].眼科新进展,2016,36(11):1020-1023.[doi:10.13389/j.cnki.rao.2016.0272]
　LI Lan-Gen,WEI Wei,ZHANG Yu-Feng,et al.Protective effects of STAT3 on retinal pigmented epithelium cells against oxidative stress[J].Recent Advances in Ophthalmology,2016,36(11):1020-1023.[doi:10.13389/j.cnki.rao.2016.0272]
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信号转导/转录激活因子3抗ARPE-19细胞氧化应激研究
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年11期
页码:
1020-1023
栏目:
实验研究
出版日期:
2016-11-05

文章信息/Info

Title:
Protective effects of STAT3 on retinal pigmented epithelium cells against oxidative stress
作者:
李兰根; 伟伟; 张玉凤; 格日乐图
010017　内蒙古自治区呼和浩特市，内蒙古自治区人民医院眼科
Author(s):
LI Lan-Gen ; WEI Wei;  ZHANG Yu-Feng; GERILETU
Department of Ophthalmology , Inner Monogolia People’s Hospital , Huhehaote 010017 , Inner Monogolia Automy Region , China
关键词:
氧化低密度脂蛋白; 信号转导/转录激活因子3; 人视网膜色素上皮细胞-19; 细胞衰老; 活性氧族
Keywords:
oxidized low density lipoprotein;  signal transduction/ activation of transcription factor 3 ;  human retinal pigment epithelial cells-19 ;  cell senescence ;  reactive oxygen species
分类号:
Ｒ７７４
DOI:
10.13389/j.cnki.rao.2016.0272
文献标志码:
A
摘要:
目的　本研究旨在观察信号转导／转录激活因子３（ｓｉｇｎａｌｔｒａｎｓｄｕｃｔｉｏｎ／ａｃｔｉｖａｔｉｏｎｏｆｔｒａｎｓｃｒｉｐｔｉｏｎｆａｃｔｏｒ３，ＳＴＡＴ３）对视网膜色素上皮细胞（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍｃｅｌｌｓ，ＲＰＥ）氧化应激损伤的保护作用，并探讨ＳＴＡＴ３在年龄相关性黄斑变性（ａｇｅ-ｒｅ-ｌａｔｅｄｍａｃｕｌａｒｄｅｇｅｎｅｒａｔｉｏｎ，ＡＭＤ）发病机制中的意义。方法　体外培养ＡＲＰＥ-１９细胞系，氧化低密度脂蛋白及Ｈ２Ｏ２干预培养细胞诱导氧化应激损伤，通过对细胞增殖、凋亡、活性氧族（ｒｅａｃｔｉｖｅｏｘｙｇｅｎｓｐｅｃｉｅｓ，ＲＯＳ）水平及细胞衰老分析研究，评估氧化应激损伤对ＲＰＥ的影响；实时荧光定量技术分析氧化应激过程中ＳＴＡＴ３-ｍＲＮＡ表达状况；ＳＴＡＴ３过表达载体转染ＡＲＰＥ-１９细胞，烟酰胺预处理细胞再经Ｈ２Ｏ２及氧化低密度脂蛋白干预后通过对增殖、凋亡、ＲＯＳ及细胞衰老状态的分析，了解ＳＴＡＴ３抗ＲＰＥ氧化应激效果。结果　与对照组相比，Ｈ２Ｏ２和氧化低密度脂蛋白能显著增加ＲＯＳ水平，促使细胞衰老增加，细胞的增殖显著下降而细胞凋亡显著上升（均为Ｐ＜０．０５）。氧化应激状况下ＳＴＡＴ３上游产物表达上升，氧化低密度脂蛋白及Ｈ２Ｏ２组荧光表达强度分别是３．３±１．２及３．５±１．１，与对照组相比差异均有统计学意义（均为Ｐ＜０．０５）；ＳＴＡＴ３能保护ＡＲＰＥ-１９细胞抗氧化应激损伤，使细胞增殖增加、凋亡减少及ＲＯＳ累积，但不造成细胞衰老状况加剧，说明ＡＲＰＥ-１９细胞衰老不受ＳＴＡＴ３调节。结论ＳＴＡＴ３在细胞氧化损伤中能独立地发挥抗氧化应激作用，提示了ＳＴＡＴ３在ＡＭＤ治疗过程中的应用前景。
Abstract:
Objective To observe the protective effects of signal transduction/activation of transcription factor 3 ( STAT3 ) on retinal pigment epithelial ( RPE ) cells against oxidative stress, and explore its role in the pathogenesis of age-related macular degeneration ( AMD) . Methods APRE-19 cell lines were cultured in vitro and the oxidative stress injury was induced by oxidized low density lipoprotein ( ox-LDL) and H202. The rates of proliferation and apoptosis, levels of intracellular reactive oxygen species (ROS) and cell senescence of RPEs were evaluated. qRT-PCR technology was used to assess the STAT3-mRNA. Furthermore. STAT3 0verexpressed in ARPE-19 cells and the cells were pre-treated with nicotinamide .and interfered with H202 and ox-LDL. The proliferation , apoptosis , ROS level and cell senescence were analyzed to know the effects of STAT3 0n RPE against oxidative stress. Results Compared with the control group , H2 02 and ox-LDL could increase the ROS level and promote the cell senescence . and the proliferation was obviously decreased , and the apoptosis significantly increased ( all P < 0. 05 ) . Under oxidative stress . the STAT3-mRNA expression was increased and the expression density of ox-LDL and H_ Oz were 3. 3 + 1. 2 and 3. 5 + 1. I , respectively , there were statistical differences compared with control group ( all P < 0. 05 ) . STAT3 could protect cells from oxidative stress damage ,increased cell proliferation. decreased apoptosis and ROS accumulation; But could not modulate the cell senescence during oxidative stress. Conclusion STAT3 can anti-oxidative stress damage independently, and prompt the application of STAT3 in the treatment of AMD.

相似文献/References:

[1]李雷,杨名珠,周庆儒,等.TLR-4/MyD88通路激活和小胶质细胞极化在氧化低密度脂蛋白导致的小鼠视网膜损伤中的作用[J].眼科新进展,2022,42(8):597.[doi:10.13389/j.cnki.rao.2022.0122]
　LI Lei,YANG Mingzhu,ZHOU Qingru,et al.Effect of Toll-like receptor-4/myeloid differentiation factor 88 pathway activation and microglial polarization on retinal degeneration induced by oxidized low-density lipoprotein in mice[J].Recent Advances in Ophthalmology,2022,42(11):597.[doi:10.13389/j.cnki.rao.2022.0122]

备注/Memo

备注/Memo:
内蒙古自治区自然科学基金资助项目（编号： ２０１６ＭＳ０８７２）
更新日期/Last Update: 2016-11-29