[1]付俊洪,陈霞,郭雅图,等.单眼形觉剥夺对小鼠视觉发育关键期内初级视皮层V1B区tLTP的影响[J].眼科新进展,2016,36(6):512-515.[doi:10.13389/j.cnki.rao.2016.0136]
　FU Jun-Hong,CHEN Xia,GUO Ya-Tu,et al.Effects of monocular form deprivation on tLTP in VIB area of primary visual cortex in critical period of mice visual development[J].Recent Advances in Ophthalmology,2016,36(6):512-515.[doi:10.13389/j.cnki.rao.2016.0136]
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单眼形觉剥夺对小鼠视觉发育关键期内初级视皮层V1B区tLTP的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年6期
页码:
512-515
栏目:
实验研究
出版日期:
2016-06-05

文章信息/Info

Title:
Effects of monocular form deprivation on tLTP in VIB area of primary visual cortex in critical period of mice visual development
作者:
付俊洪; 陈霞; 郭雅图; 宋德胜
300020　天津市，天津医科大学眼科临床学院天津市眼科医院
Author(s):
FU Jun-Hong; CHEN Xia ;  GUO Ya-Tu; SONG De-Sheng
Clinical College of Ophthalmology , Tianjin Medical University , Tianjin Eye Hospital , Tianjin 300020 , China
关键词:
单眼形觉剥夺; 脉冲时间依赖突触可塑性; 长时程增强
Keywords:
monocular form deprivation;  spike-timÑ; ª; g dependent synaptic plasticity ;  timing long-term potentiation
DOI:
10.13389/j.cnki.rao.2016.0136
文献标志码:
A
摘要:
目的　观察在脉冲时间依赖的刺激诱导模式下单眼形觉剥夺对小鼠视觉发育关键期内初级视皮层Ｖ１Ｂ区长时程增强（ｔｉｍｉｎｇｌｏｎｇ-ｔｅｒｍｐｏｔｅｎｔｉａｔｉｏｎ，ｔＬＴＰ）的影响及其对应诱导时间窗的变化。方法　选择健康Ｃ５７ＢＬ／６小鼠２８只，随机分为正常对照组和单眼形觉剥夺组，每组１４只，单眼形觉剥夺组右眼褥式缝合３ｄ。七氟烷麻醉后，断头、取脑，置于含体积分数９５％Ｏ２和５％ＣＯ２饱和的０～４℃脑片制备液中冷却１～２ｍｉｎ，切取后部２／５脑组织，行４００μｍ冠状连续切片，在脉冲时间依赖的条件刺激诱导模式下采用红外可视膜片钳全细胞模式记录正常对照组、单眼形觉剥夺组（含剥夺侧皮层、非剥夺侧皮层）视皮层Ｖ１Ｂ区脑片Ⅱ／Ⅲ层锥体神经元ＮＭＤＡ介导的兴奋性突触后电位（ｅｘｃｉｔａｔｏｒｙｐｏｓｔｓｙｎａｐｔｉｃｐｏｔｅｎｔｉａｌｓ，ＥＰＳＰ）。结果　正常对照组小鼠脑片初级视皮层Ｖ１Ｂ区Ⅱ／Ⅲ层锥体神经元予间隔Δｔ＝１０ｍｓ的突触前后联合刺激后ＥＰＳＰ反应增加，可成功诱导ｔＬＴＰ，但当Δｔ＝１００ｍｓ则未能诱导ｔＬＴＰ［Δｔ＝＋１０ｍｓ：ＥＰＳＰ斜率（１２４．１±３．９）％；Δｔ＝＋１００ｍｓ：ＥＰＳＰ斜率（１００．４±３．３）％；Ｐ＜０．００１）。单眼形觉剥夺组小鼠剥夺侧初级视皮层分别给予Δｔ＝１０ｍｓ、１００ｍｓ的脉冲时间依赖的条件刺激均可诱导出ｔＬＴＰ［Δｔ＝＋１０ｍｓ：ＥＰＳＰ斜率（１３０．８±１．７）％；Δｔ＝＋１００ｍｓ：ＥＰＳＰ斜率（１１４．７±０．３）％；Ｐ＜０．００１）。单眼形觉剥夺组小鼠非剥夺侧视皮层分别给予Δｔ＝１０ｍｓ、５０ｍｓ的脉冲时间依赖的条件刺激均可诱导出ｔＬＴＰ［Δｔ＝＋１０ｍｓ：ＥＰＳＰ斜率（１２９．６±１．５）％；Δｔ＝＋５０ｍｓ：ＥＰＳＰ斜率（１２０．５±０．９）％］，而当Δｔ＝１００ｍｓ时，未能成功诱导ｔＬＴＰ［Δｔ＝＋１００ｍｓ：ＥＰＳＰ斜率（１０１．１±０．６）％］。结论　单眼形觉剥夺３ｄ可以改变小鼠初级视皮层Ｖ１Ｂ区兴奋性通路的脉冲时间依赖的突触可塑性。剥夺侧较非剥夺侧及正常视皮层ｔＬＴＰ诱导时间窗增宽，有助于从突触可塑性角度解释弱视内在神经突触机制。
Abstract:
Objective To investigate the effects of monocular form deprivation on timing long-term potentiation ( tLTP) in the VIB area of mouse primary visual cortex in the critical period of mice visual development under the spike-tinung dependent induction pattern.and observe the timing window change of tLTP. Methods Twentyeight healthy C57BL/6 mice were randomly divided into normal control group and monocular form deprivation group , 14 cases in each group ,the right eye of mice rn monocular form deprivation group was sutured for 3 days. After anesthetizing by sevoflurane, the brains were quickly removed to ice-cold buffer bubbled with 95% 02 and 5% C02 for I - 2 nunutes. Rear 2/5 brain tissue including the visual cortex were cut int0 400 ym brain slices. Visualized whole-cell current-clamp recordings were made from layer II/III regular-spiking pyramidal cells ( deprived side or non-deprived side) to record the excitatory postsynaptic potentials ( EPSP) . Results In normal control group , the pre-then post-pairing stimulation in layer II/III regular-spiking pyramidal cells resulted in tLTP when the delay ( At) was 10 ms( EPSP slope 124. 1% +3. g% ) .but not when At was 100 ms ( EPSP slope 100. 4% +3. 3% ) (P < 0. 001). In the deprived side of from monocular deprivation group ,both delays ( 10 ms , 100 ms) resulted in comparable tLTP ( At = + 10 ms:EPSP slope 130. 8% + 1. 7% ; At = + 100 ms : EPSP slope 114. 7% + 0. 3% ;P < 0. 001 ) . In non-deprived visual cortex , the pre-then post-pairing stimulation resulted in tLTP when the delay was 10 ms and 50 ms ( At = + 10 ms :EPSP slope 129. 6% +1. 5% ; At = +50 ms:EPSP slope 120. 5% +0. g% ) .but not when it was - 100 ms ( At = + 100 ms:EPSP slope 101. 1% + 0. 6% ). Conclusion Monocular form deprivation for 3 days can change the spike-timing dependent synaptic plasticity of excitory pathway in the VIB area of mouse primary visual cortex. The tinung window for postsynaptic tLTP of deprived visual cortex is wider than those of non-deprived and normal visual cortex. which may help to explain the underlying synapse mechanism of monocular deprived amblyopia.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：３０７３００９９）；天津市应用基础与前沿技术研究计划青年项目（编号：１４ＪＣＱＮＪＣ１０５００）
更新日期/Last Update: 2016-06-27