[1]康剑书,曾颖,赵灿,等.不同波长色光环境下恒河猴视网膜及巩膜视黄酸含量的变化[J].眼科新进展,2016,36(3):216-219.[doi:10.13389/j.cnki.rao.2016.0058]
　KANG Jian-Shu,ZENG Ying,ZHAO Can,et al.Differences of retinoic acid level in retina and sclera of thesus monkey under different wavelengths of colored light[J].Recent Advances in Ophthalmology,2016,36(3):216-219.[doi:10.13389/j.cnki.rao.2016.0058]
点击复制

不同波长色光环境下恒河猴视网膜及巩膜视黄酸含量的变化
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年3期
页码:
216-219
栏目:
实验研究
出版日期:
2016-03-05

文章信息/Info

Title:
Differences of retinoic acid level in retina and sclera of thesus monkey under different wavelengths of colored light
作者:
康剑书; 曾颖; 赵灿; 杨骁; 何彬; 钟华
650031　云南省昆明市，昆明医科大学第一附属医院眼科
Author(s):
KANG Jian-Shu ; ZENG Ying ;  ZHAO Can ;  YANG Xiao;  HE Bin ;  ZHONG Hua
Department of Ophthatmology , the First Affiliated Hospital of Kunming Medical University, Kunming 650031 , Yunan Province , China
关键词:
视黄酸; 恒河猴; 色光
Keywords:
retinoic acid ;  thesus monkey ;  colored light
DOI:
10.13389/j.cnki.rao.2016.0058
文献标志码:
A
摘要:
目的　探讨不同波长的色光环境对恒河猴视网膜及巩膜中视黄酸（ｒｅｔｉｎｏｉｃａｃｉｄ，ＲＡ）含量的影响。方法　２０只５４ｄ龄的恒河猴随机分为５组（每组４只）：紫光灯组、荧光灯组、白炽灯组、红光灯组及全光谱组，进行每天１２ｈ的光照（早８点至晚８点）。于光照２４周后处死，同一天上午无菌操作取材，取鼻上方视网膜及巩膜组织。取空白样品，加入全反ＲＡ标准溶液，按样品处理方法操作后，绘制标准曲线，根据标准品的色谱分析，分别确定出各组织ＲＡ的出峰位置，根据计算机的色谱工作站计算出样品的含量，以全光谱组为对照组。结果　恒河猴眼视网膜、巩膜中均有ＲＡ的存在；视网膜及巩膜中ＲＡ含量间差异无统计学意义。光照２４周后，红光灯组及全光谱组视网膜ＲＡ含量均高于紫光灯组、荧光灯组、白炽灯组（均为Ｐ＜０．０５）。红光灯组与全光谱组视网膜ＲＡ含量间差异无统计学意义；红光灯组与全光谱组巩膜ＲＡ含量高于紫光灯组，差异均有统计学意义（均为Ｐ＜０．０５）。余各组间两两比较差异均无统计学意义。结论　恒河猴视网膜及巩膜ＲＡ含量无明显差异；视网膜中ＲＡ的含量在长波长色光环境下表达增高，在短波长色光环境下表达减少；巩膜与视网膜ＲＡ含量的变化基本一致，提示ＲＡ可能在不同色光环境下近视的形成过程中发挥一定作用。
Abstract:
Objective To study the differences of the retinoic acid ( RA) level in the retina and sclera of thesus monkey under different wavelengths of colored light environment. Methods Five groups of thesus monkeys (54 days of age,4 cases in each group) were raised for 24 weeks under different wavelengths of colored light ,involving purple lamp light ( PL) group , fluorescent lamp light ( FL) group , incandescent lamp light ( IL) group ,red lamp light ( RL) group , and full spectrum light ( FSL) group , all for 12 hours per day ( from 8 : 00 AM t0 8 : 00 PM). The nasal retinal and sclerotic tissues were collected after 24 weeks of feeding on the same day in the morning , standard solution of RA was added into blank sample , then the standard curve was drew. The peak position of RA in all tissues was found according to chromatography analysis of control standard substance. Finally, the levels of RA in samples were calculated by computer chromatography workstation. FSL group was regarded as control group. Results RA could be found in retina and sclera in the thesus monkeys. The level of RA in sclera was similar with that of in the retina(P > 0. 05 ) . After 24 weeks of feeding , the RL and FSL group had a higher RA level in retina as compared to that of PL , FL and IL groups ( all P < 0. 05 ) . There was no significant difference in the RA level between the RL group and FSL group(P > 0. 05 ) . The RL and FSL group had a higher RA level in sclera as compared to that of PL group (P < 0. 05 ) . There was no sigruficant difference between other groups. Conclusion RA can be found in retina and sclera in the thesus monkeys. The expression of RA in the retina increase under the long wavelength light environment. and reduce when under the short wavelength light environment. The changes of RA content in the sclera are consistent with that in the retina.which indicate that RA may play the certain role in the development of myopia.

相似文献/References:

[1]朱勤 胡竹林 杨忠昆.恒河猴血管内皮细胞移植替代角膜内皮细胞后房水血管内皮生长因子浓度变化的观察[J].眼科新进展,2012,32(1):000.
[2]张虹 朱勤 胡竹林.BrdU示踪的恒河猴血管内皮细胞移植替代角膜内皮细胞的免疫组化染色观察[J].眼科新进展,2012,32(12):000.
[3]朱勤 肖中男 孙晓梅 胡敏 刘海 胡竹林.超声乳化致恒河猴角膜内皮损伤动物模型的建立[J].眼科新进展,2013,33(2):000.
[4]李传旭 崔冬梅 霍丽君 項道满 曾骏文.外源性视黄酸诱导的豚鼠近视模型的建立[J].眼科新进展,2012,32(8):000.
[5]朱勤 肖中男 孙晓梅 胡敏 刘海 胡竹林.恒河猴血管内皮细胞移植替代角膜内皮细胞后房水中离子浓度的变化[J].眼科新进展,2013,33(4):000.
[6]朱勤 孙晓梅 胡敏 刘海 胡竹林.利用离心沉淀法移植恒河猴血管内皮细胞替代角膜内皮的可行性分析[J].眼科新进展,2013,33(5):000.
[7]张文佳,胡竹林,朱勤,等.人脐静脉血管内皮细胞在体移植替代猴角膜内皮细胞的形态学分析[J].眼科新进展,2014,34(11):1020.[doi:10.13389/j.cnki.rao.2014.0283]
　ZHANG Wen-Jia,HU Zhu-Lin,ZHU Qin,et al.Microstructure observation of human vascular endothelial cells transplantation in autograft to replace monkey corneal endothelial cells[J].Recent Advances in Ophthalmology,2014,34(3):1020.[doi:10.13389/j.cnki.rao.2014.0283]
[8]戴锦晖. 色觉在眼屈光发育中的作用[J].眼科新进展,2015,35(2):101.[doi:10.13389/j.cnki.rao.2015.0027]
　DAI Jin-Hui. Role of color vision in development of ocular refraction[J].Recent Advances in Ophthalmology,2015,35(3):101.[doi:10.13389/j.cnki.rao.2015.0027]
[9]李戴弟,高云霞,潘玲珍,等.Lucentis和Avastin治疗激光诱导恒河猴脉络膜新生血管模型的作用[J].眼科新进展,2016,36(5):423.[doi:10.13389/j.cnki.rao.2016.0113]
　LI Dai-Di,GAO Yun-Xia,PAN Ling-Zhen,et al.Therapeutic effects of Avastin and Lucentis on laser-induced choroidal neovascularization in thesus monkeys[J].Recent Advances in Ophthalmology,2016,36(3):423.[doi:10.13389/j.cnki.rao.2016.0113]
[10]马瑞杰,李妍,孙晓梅,等.改良揭膜消化法分离培养恒河猴角膜内皮细胞[J].眼科新进展,2016,36(7):618.[doi:10.13389/j.cnki.rao.2016.0164]
　MA Rui-Jie,LI Yan,SUN Xiao-Mei,et al.Modified "peel-and-digest" procedure for isolation and culture corneal endothelial cells in Rhesus monkeys[J].Recent Advances in Ophthalmology,2016,36(3):618.[doi:10.13389/j.cnki.rao.2016.0164]

备注/Memo

备注/Memo:
国家自然科学基金项目（编号：３０９６０４１４）
更新日期/Last Update: 2016-03-08