[1]姜双,陈晓隆.HMGB1和TOLL样受体9在糖尿病大鼠视网膜组织中的表达[J].眼科新进展,2016,36(2):101-104.[doi:10.13389/j.cnki.rao.2016.0028]
　JIANG Shuang,CHEN Xiao-Long.Expression of HMGBl and TLR9 in retina of diabetic rats[J].Recent Advances in Ophthalmology,2016,36(2):101-104.[doi:10.13389/j.cnki.rao.2016.0028]
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HMGB1和TOLL样受体9在糖尿病大鼠视网膜组织中的表达
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年2期
页码:
101-104
栏目:
实验研究
出版日期:
2016-02-05

文章信息/Info

Title:
Expression of HMGBl and TLR9 in retina of diabetic rats
作者:
姜双; 陈晓隆
121000　辽宁省锦州市，辽宁医学院附属第三医院眼科
Author(s):
JIANG Shuang ; CHEN Xiao-Long
Department of Ophthalmology,the Third Affiliated Hospital of Liaoning Medical University. Jinzhou 121000 .Liaoning Province, China
关键词:
糖尿病视网膜病变; HMGB1; TOLL样受体9
Keywords:
diabetic retinopathy ;  HMGBI ;  Toll-Iike receptor 9
DOI:
10.13389/j.cnki.rao.2016.0028
文献标志码:
A
摘要:
目的　探讨ＨＭＧＢ１和ＴＯＬＬ样受体（Ｔｏｌｌ-ｌｉｋｅｒｅｃｅｐｔｏｒｓ，ＴＬＲ）９在糖尿病大鼠视网膜中的表达。方法　采用链脲佐菌素腹腔注射的方法制作糖尿病大鼠模型，于注药后４周、８周、１２周处死大鼠，取视网膜进行ＨＥ染色和免疫组织化学染色检测，并与正常对照组大鼠作对比。结果　ＨＭＧＢ１和ＴＬＲ９在正常对照组和糖尿病４周、８周、１２周时的光密度值分别为０．１０３７±０．００１１、０．１３２３±０．０００５、０．１４５０±０．００２０、０．１５５７±０．００１５和０．０８４３±０．００４７、０．１１６４±０．００９４、０．１３２１±０．０００１、０．１３９０±０．００４２，在糖尿病大鼠视网膜中的表达呈时间依赖性增加，主要表达在神经节细胞层、内核层和外核层，与正常对照组相比差异均有统计学意义（均为Ｐ＜０．０１）。结论　ＨＭＧＢ１和ＴＬＲ９在糖尿病大鼠视网膜中表达增加，ＨＭＧＢ１-ＴＬＲ９信号通路可能参与了糖尿病视网膜病变的发生发展。
Abstract:
Objective To investigate the expressions of HMGBI and Toll-Iike receptor 9 (TLR9 ) in the retina of diabetic rats. Methods Diabetic rat model was established by intraperitioneal injection of STZ. At 4 weeks ,8 weeks and 12 weeks after injection , the rats were sacrificed and eyeballs were enucleated for HE stairung and immunohistochemistry, and the results were compared with the normal rat. Results OD value of HMGBI and TLR9 in the retinas of diabetic rats at 4 weeks,8 weeks and 12 weeks were 0. 103 7 +0. 001 1.0. 132 3 +0. 000 5 .0. 145 0 + 0. 002 0 .0. 155 7 +0. 001 5 and 0. 084 3 +0. 004 7 ,0. 116 4 + 0. 009 4 ,0. 132 I + 0. 000 1.0. 139 0 + 0. 004 2 . which were sigruficantly increased compared with the normal control ( all P < 0. 01 ). Both were mainly expressed in the ganglion cell layer,inter nuclear layer and outer nuclear layer. Conclusion The expressions of HMGBI and TLR9 in the retina of diabetic rats increase , and the HMGBI-TLR9 signaling pathway may be involved in the pathogenesis of diabetic retinopathy.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：８１５７０８６６）；辽宁医学院青年启动资金项目（编号：ｙ２０１１ｚ００６）
更新日期/Last Update: 2016-01-29