[1]陈艳,孙冰,唐丽琴. 那他霉素粉剂和滴眼液在兔眼角膜和房水中表达的实验研究[J].眼科新进展,2015,35(7):626-629.[doi:10.13389/j.cnki.rao.2015.0170]
　CHEN Yan,SUN Bing,TANG Li-Qin. Expression of natamycin eye drops and powder in rabbits cornea and aqueous humor[J].Recent Advances in Ophthalmology,2015,35(7):626-629.[doi:10.13389/j.cnki.rao.2015.0170]
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 那他霉素粉剂和滴眼液在兔眼角膜和房水中表达的实验研究
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年7期
页码:
626-629
栏目:
实验研究
出版日期:
2015-07-05

文章信息/Info

Title:
 Expression of natamycin eye drops and powder in rabbits cornea and aqueous humor
作者:
 陈艳; 孙冰; 唐丽琴
 230001　安徽省合肥市，安徽省立医院眼科
Author(s):
 CHEN Yan; SUN Bing; TANG Li-Qin
 Department of Ophthalmology , Anhui Provincial Hospital, Hefei 230001 , Anhui Province . China
关键词:
 ; 那他霉素; 高效液相色谱法; 药代动力学; 角膜; 房水
Keywords:
 ; natamycin ;  high performance liquid chromatography ;  pharmacokinetics ;  cornea ;  aqueous humor
DOI:
10.13389/j.cnki.rao.2015.0170
文献标志码:
A
摘要:
 目的　研究那他霉素粉末直接给药和滴眼液给药在兔眼角膜的药代动力学的差异。方法　将１６只新西兰白兔随机分为Ａ、Ｂ两组，每组８只，每只白兔左右眼分别使用那他霉素干粉（每１０ｍｉｎ涂１次）和那他霉素眼液（每５ｍｉｎ滴２０μＬ）。２ｈ后Ａ组取其角膜，Ｂ组抽取房水。运用高效液相色谱法分别测定那他霉素含量，柱温为３０℃，流动相为乙酸铵溶液-乙腈-四氢呋喃，流速为１ｍＬ?ｍｉｎ－１，检测波长为３０３ｎｍ。结果　在该色谱条件下，那他霉素的保留时间为６．５ｍｉｎ，分离度良好，标准曲线在０．０５～５０．００ｍｇ?Ｌ－１内线性关系良好（ｒ＝１．０００），最低定量限为０．０５ｍｇ?Ｌ－１。Ａ组经那他霉素粉末给药的角膜中药物浓度为（１９．３９±１．９５）ｍｇ?Ｌ－１，较滴眼液给药组的（１０．３４±３．０２）ｍｇ?Ｌ－１显著增高，差异有显著统计学意义（Ｐ＜０．００１）；Ｂ组经粉末给药的房水中药物浓度为（１．６７±０．１５）ｍｇ?Ｌ－１，较滴眼液给药组的（１．４６±０．１４）ｍｇ?Ｌ－１明显增高，差异有统计学意义（Ｐ＝０．０１３）。结论那他霉素粉末在兔眼角膜的穿透性高于那他霉素滴眼液。
Abstract:
 Objective To study the differences of pharmacokinetics in the rabbit cornea of natamycin powder administration directly and eye drops administration. Methods Sixteen New Zealand rabbits were randomly divided into two groups , each group consisted of 8 rabbits. The left and right eye of every rabbit was adnunistrated with natamycin eye drops (20 VL per 5 nunutes) and natamycin powder ( once per 10 minutes) respectively. The corneal tissue in group A was taken , and the aqueous humor in group B was extracted. Natamycin content was determined by high performance liquid chromatography(HPLC). The column temperature was 30 9C . the mobile phase of ammonium acetate solution-acetonitrile-tetrahydrofuran, the flow rate was I mL . min’1 ,the detection wavelength was 303 nm. Results Under the chromatographic conditions,natamycin retention time was 6. 5 minutes, separation was good, standard curve within 0. 05 - 50. 00 mg . L-I had good linear relationship ( r = 1. 000 ) , limit of quantification was 0. 05 mg . L -’. Natamycin concentration in cornea adnunistrated with powder [ ( 19. 39 + 1. 95 ) mg . L-l ] was much higher than that administrated with eye drops [ ( 10. 34 + 3. 02) mg . L -l ] (P < 0. 001 ) ; Natamycin concentration in aqueous humor adnunistrated with powder [ ( 1. 67 +0. 15 ) mg . L-l ] was also higher than that administrated with eye drops [ (1. 46 +0. 14) mg . L-I ] (P = 0. 013). Conclusion The penetrability of natamycin powder in rabbit cornea is higher than that of eye drops.

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备注/Memo

备注/Memo:
 安徽医科大学校科学研究基金资助（编号：２０１２ｘｋｊ０３８）
更新日期/Last Update: 2015-07-03