[1]郭惠玲,杜改萍,王大江,等. 新型免疫抑制剂J2纳米混悬液抑制大鼠角膜移植排斥反应的实验研究[J].眼科新进展,2015,35(7):608-611.[doi:10.13389/j.cnki.rao.2015.0165]
　GUO Hui-Ling,DU Gai-Ping,WANG Da-Jiang,et al. Study on a novel immunosuppressant of J2 nano-suspension on rat corneal transplantation rejection[J].Recent Advances in Ophthalmology,2015,35(7):608-611.[doi:10.13389/j.cnki.rao.2015.0165]
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 新型免疫抑制剂J2纳米混悬液抑制大鼠角膜移植排斥反应的实验研究
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年7期
页码:
608-611
栏目:
实验研究
出版日期:
2015-07-05

文章信息/Info

Title:
 Study on a novel immunosuppressant of J2 nano-suspension on rat corneal transplantation rejection
作者:
 郭惠玲; 杜改萍; 王大江; 胡莲娜; 伍春荣; 高付林; 闫洪欣; 黄一飞
 100101　北京市，解放军３０６医院眼科
Author(s):
 GUO Hui-Ling ;  DU Gai-Ping ;  WANG Da-Jiang;  HU Lian-Na ;  WU Chun-Rong ; GAO Fu-Lin ; YAN Hong-Xin ;  HUANG Yi-Fei
 Department of Ophthalmology , Chinese PLA 306 Hospital , Beijing 100101 , China
关键词:
 ; 免疫抑制剂; 角膜移植; 排斥反应; 颌下淋巴结
Keywords:
 ; immunosuppressant ;  corneal transplantation ;  rej ection ;  submandibular lymph nodes
DOI:
10.13389/j.cnki.rao.2015.0165
文献标志码:
A
摘要:
 目的　观察计算机辅助药物设计的新型免疫抑制剂Ｊ２纳米混悬液（ｎａｎｏ-ｓｕｓｐｅｎ-ｓｉｏｎ，ＮＳ）结膜下注射对大鼠角膜移植排斥反应的抑制作用。方法　成年雌性Ｗｉｓｔａｒ大鼠为供体，Ｓｐｒａｇｕｅ-Ｄａｗｌｅｙ（ＳＤ）大鼠为受体，建立同种异体穿透性角膜移植模型。手术大鼠随机分成３组，每组各１６只（３２眼），Ａ组：ＳＤ大鼠自体角膜移植，结膜下注射空白溶剂０．０５ｍＬ；Ｂ、Ｃ组为同种异体角膜移植组，术后分别给予结膜下注射空白溶剂０．０５ｍＬ及１０ｇ?Ｌ－１Ｊ２ＮＳ。术后记录角膜植片排斥指数（ｒｅｊｅｃｔｉｏｎｉｎｄｅｘ，ＲＩ），当ＲＩ≥６，确定为排斥。各组于角膜移植后７ｄ、１４ｄ随机选取２只行术眼及同侧颌下淋巴结病理学检查。结果　Ａ组：术后２～３ｄ角膜植片由透明逐渐变为混浊、水肿，随后保持透明。Ｂ组：术后３～５ｄ角膜植片开始水肿、混浊，７～１０ｄ时水肿、混浊发展迅速。Ｃ组：术后１周反应与Ａ组相似，之后出现角膜水肿、混浊，术后９～１４ｄ角膜完全混浊。Ａ、Ｂ、Ｃ组的角膜植片生存时间分别为（３０．００±０．００）ｄ、（１０．７５±１．６０）ｄ、（１４．３３±２．９３）ｄ，三组间差异有统计学意义（均为Ｐ＜０．０５）。组织学观察发现：Ａ组术后炎症反应轻微，基质厚度基本正常或增厚，内皮层完整。Ｂ组术后１４ｄ时可见典型的免疫排斥反应，植片角膜上皮空泡样改变、部分崩解坏死。Ｃ组较Ｂ组角膜植片中细胞成分和新生血管均少，同侧颌下淋巴结增生明显减弱。结论　Ｊ２ＮＳ结膜下注射有抑制大鼠角膜移植排斥的作用，可减轻角膜移植术后角膜植片和同侧颌下淋巴结的细胞增生，抑制免疫应答。
Abstract:
 Objective To investigate the inhibition on the rejection of rat corneal transplantation of novel immunosuppressant of J2 nano-suspension ( J2-NS) . Methods Allogeneic penetrating keratoplasty model was established by using the adult female Wistar rats as donors and Sprague-Dawley ( SD) rats as recipients. Rats were divided int0 3 groups , 16 cases ( 32 eyes) in each group. Group A: SD rats were injected 0. 05 mL placebo subconjunctivally after autograft keratoplasty. Group B : Allograft rats were injected 0. 05 mL placebo subconjunctivally after surgery. Group C :Allograft rats were injected 10 g . L-l J2-NS 0. 05 mL subconjunctivally after surgery. The graft rejection index ( RI) and survival time of animals in each group were recorded. If the RI was more than 6 , the rejected reaction happened. The cornea and ipsilateral submandibular lymph nodes were exanuned pathologically at 7 days , 14 days. respectively , after surgery. Results Group A : The grafts had a little edema and opaque from 2 days t0 3 days postoperatively , while after the graft remained transparency. Group B : The grafts became edema and opaque from 3 days t0 5 days postoperatively, and the degree of the edema and opaque became speedy from 7 days t0 10 days postoperatively. Group C : The reaction was similar with group A at I week postoperatively , while after the graft became edema and opaque. The graft rejected reaction happened from 9 days t0 14 days. The survival time of group A,B,C were ( 30. 00 +0. 00 ) days , ( 10. 75 + 1. 60) days and ( 14. 33 + 2. 93 ) days ,there were significant differences between each two groups ( all P < 0. 05 ) . Histologiy showed that the inflammation was slightly , the stroma was normal and the endothelium was intact in group A;The appearance of rejection appeared at 14 days,the corneal epithelium appeared vacuole and necrosis partly in group B. The cellular components and angiogenesis in all layers of cornea of group C were less compared with group B at the same period. The hyperplasia of ipsilateral submandibular lymph nodes were also weaker than group B. Conclusion Subconjunctival injection of J2-NS can inhibit rat corneal allograft rejection,reduce cell proliferation and inhibit the immune response in cornea and ipsilateral submandibular lymph nodes.

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备注/Memo

备注/Memo:
 国家自然科学基金资助（编号：３０９７３２４５）；全国博士后科学基金面上项目（编号：２０１００４８１４８１）
更新日期/Last Update: 2015-07-03