j.cnki.rao.2015.0119]
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泪腺良性淋巴上皮病变免疫组织化学研究

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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

Title:: Immunohistochemical research on benign lymphoepithelial lesion of lacrimal gland

Author(s):: LI Jing; GE Xin; MA Jian-Min; Beijing Tongren Eye Center, Beijing Tongren Hospital,Capital Medical University,Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing 100730．China

Keywords:: ; benign lymphoepithelial lesion ; lacrimal gland ; orbital disease ; immunohistochemical staining

摘要:: 目的　通过研究９种单克隆抗体在泪腺良性淋巴上皮病变中的表达，探讨其免疫病理本质及发病机制，为临床治疗及预后提供理论依据。方法　使用ＣＤ４、ＣＤ８、ＣＤ２１、ＣＤ３４、ＣＤ４５、ＣＤ６８、ＩｇＧ、ＩｇＧ４和增殖细胞核抗原这９种分子的单克隆抗体，应用免疫组织化学染色ＳＰ法对２５例泪腺良性淋巴上皮病变的石蜡标本进行免疫组织化学染色。结果Ｂ淋巴细胞表面抗原（ＣＤ２１）、Ｔ淋巴细胞表面抗原（ＣＤ４、ＣＤ８和ＣＤ４５）、黏附分子ＣＤ３４及ＩｇＧ在２５例样本中均呈阳性表达，巨噬细胞表面抗原ＣＤ６８、增殖细胞核抗原和ＩｇＧ４这３种分子在２５例样本中均有一定比例的阳性表达，表达率分别为１２％、８％和８０％。结论泪腺良性淋巴上皮病变是一种主要以Ｂ淋巴细胞和Ｔ淋巴细胞激活为主要病理免疫基础的疾病，绝大多数属于ＩｇＧ４相关性疾病的范畴，同时有发生恶变的潜能，临床治疗后的密切随访很有必要。

Abstract:: Objective To investigate the expression of nine monoclonal antibodies in benign lymphoepithelial lesion ( BLEL) of lacrimal gland , and explore the immunopathological essences and mechanisms involved in BLEL of lacrimal gland. Methods Using SP immunohistochemistry analysis , twenty-five cases of BLEL of lacrimal gland were studied by using a panel of rune monoclonal antibodies, including CD4, CD8 , CD21 , CD34 ,CD45 , CD68 , IgG ,IgG4 and PCNA. Results Surface antigens of B cells ( CD21) ,T cells( CD4 ,CD8 and CD45 ) , adhesion molecule ( CD34) and immunoglobulinG (IgG) were all positively stained in all 25 cases. surface antigens of macrophages ( CD68 ) , proliferative cell nuclear antigen( PCNA) and immunoglobulinG4 ( IgG4 ) were positively stained in different proportion of cases, which were 12% , 8% and 80% , respectively. Conclusion BLEL of lacrimal gland is a disease mainly pathologically featured by the activation of both B lymphocytes and T lymphocytes . the vast majority of which is belong to the IgG4-related disease. Due to its potential possibility of malignant transformation.closely follow up is necessary after the clinical treatment.

更新日期/Last Update: 2015-04-27